A5052600707- biodegradable polymer synthesis and properties
- 3D Printing in Biomedical Research
- Microbial Metabolic Engineering and Bioproduction
- Microplastics and Plastic Pollution
- Bioinformatics and Genomic Networks
- Additive Manufacturing and 3D Printing Technologies
- Microbial Natural Products and Biosynthesis
- Carbon dioxide utilization in catalysis
- Electrospun Nanofibers in Biomedical Applications
- Listeria monocytogenes in Food Safety
- Fuel Cells and Related Materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Catalysis and Immobilization
- Mechatronics Education and Applications
- Polymer crystallization and properties
- Spectroscopy Techniques in Biomedical and Chemical Research
- Experimental Learning in Engineering
- Antioxidant Activity and Oxidative Stress
- Genomics, phytochemicals, and oxidative stress
- Biopolymer Synthesis and Applications
- Moringa oleifera research and applications
- Viral Infectious Diseases and Gene Expression in Insects
- Biomedical and Engineering Education
- Spectroscopy and Chemometric Analyses
Biocat
2023
Technische Universität Berlin
2018-2023
Leibniz Institute of Vegetable and Ornamental Crops
2019
Bioconversion of waste animal fat (WAF) to polyhydroxyalkanoates (PHAs) is an approach lower the production costs these plastic alternatives. However, solid nature WAF requires a tailor-made process development. In this study, double-jacket feeding system was built thermally liquefy employ continuous strategy. During laboratory-scale cultivations with Ralstonia eutropha Re2058/pCB113, 70% more PHA (45 gPHA L-1 ) and 75% higher space-time yield (0.63 h-1 were achieved compared previously...
Biodegradable and biocompatible polyhydroxyalkanoates (PHAs) are promising alternatives to conventional plastics. Based on the chain length of their monomers they classified as short (scl-) or medium (mcl-) PHA polymers. The type monomers, composition molecular weight (MW) define polymer properties. To accelerate use a bulk material, downstream associated costs need be minimized. This study focuses evaluation non-halogenated solvents, especially acetone scl-PHA non-solvent, for recovery...
Polyhydroxyalkanoates (PHAs) are biodegradable plastic-like materials with versatile properties. Plant oils excellent carbon sources for a cost-effective PHA production, due to their high content, large availability, and comparatively low prices. Additionally, efficient process development control is required competitive which can be facilitated by on-line or in-line monitoring devices. To this end, we have evaluated photon density wave (PDW) spectroscopy as new analytical technology...
The rapid accumulation of crude-oil based plastics in the environment is posing a fundamental threat to future mankind. biodegradable and bio-based polyhydroxyalkanoates (PHAs) can replace conventional plastics, however, their current production costs are not competitive therefore prohibiting PHAs from fulfilling potential.Different low-quality animal by-products, which were separated by thermal hydrolysis into fat-, fat/protein-emulsion- mineral-fat-mixture- (material with high ash content)...
Pak choi (Brassica rapa subsp. chinensis) is a leafy vegetable that widely available in Asia and consumed rising quantities Europe. contains high levels of secondary plant metabolites, such as carotenoids, chlorophylls, glucosinolates, phenolic compounds, vitamin K, which are beneficial for humans if on regular basis. The evaluation the genotype-induced variation metabolites revealed cultivar 'Amur' contained highest concentration metabolites. Furthermore, steaming retained more acids...
Fat-containing animal by-product streams are locally available in large quantities. Depending on their quality, they can be inexpensive substrates for biotechnological processes. To accelerate industrial polyhydroxyalkanoate (PHA) bioplastic production, the development of efficient bioprocesses that based is a promising approach to reduce overall production costs. However, solid nature requires tailor-made process development. In this study, fat/protein-emulsion (FPE), which stream from...
Process engineering of biotechnological productions can benefit greatly from comprehensive analysis microbial physiology and metabolism. Ralstonia eutropha (syn. Cupriavidus necator) is one the best studied organisms for synthesis biodegradable polyhydroxyalkanoate (PHA). A metabolomic study during bioreactor cultivations with wild-type (H16) an engineered (Re2058/pCB113) R. strain short- or medium-chain-length PHA has been carried out. production plant oil was triggered through nitrogen...
With generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is fastest growing non-pathogenic bacterium isolated so far. The availability full genome and genetic engineering tools its ability to utilize a wide range carbon sources make V. an attractive host for biotechnological production processes. However, high-cell-density cultivations, which are desired at industrial-scale have not been described far.In this study we report fed-batch cultivations...
Abstract An efficient monitoring and control strategy is the basis for a reliable production process. Conventional optical density (OD) measurements involve superpositions of light absorption scattering, results are only given in arbitrary units. In contrast, photon wave (PDW) spectroscopy dilution‐free method that allows independent quantification both effects with defined For first time, PDW was evaluated as novel process analytical technology tool real‐time biomass formation Escherichia...
Abstract Wie biobasierte und bioabbaubare Kunststoffe im Bioreaktor entstehen, lässt sich mit Photonendichtewellenspektroskopie beobachten. Ein Beispiel ist die Produktion von Polyhydroxyalkanoaten; mikrobielle Synthese dieser Polymere so inline untersuchen.
Abstract A significant challenge in our understanding of biological systems is the high number genes with unknown function many genomes. The fungal genus Aspergillus contains important pathogens humans, model organisms, and microbial cell factories. niger used to produce organic acids, proteins, a promising source new bioactive secondary metabolites. Out 14,165 open reading frames predicted A. genome only 2% have been experimentally verified over 6,000 are hypothetical. Here we show that...