A5005370263- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Zeolite Catalysis and Synthesis
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Proteomics Techniques and Applications
- Inflammatory mediators and NSAID effects
- Mass Spectrometry Techniques and Applications
- Eicosanoids and Hypertension Pharmacology
- Antiplatelet Therapy and Cardiovascular Diseases
- Biosimilars and Bioanalytical Methods
- Mesoporous Materials and Catalysis
- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Catalytic Processes in Materials Science
- Cancer-related molecular mechanisms research
- Catalysis and Hydrodesulfurization Studies
- Drug Transport and Resistance Mechanisms
- RNA and protein synthesis mechanisms
- Ferroptosis and cancer prognosis
- Cancer, Stress, Anesthesia, and Immune Response
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Cellular transport and secretion
Merck & Co., Inc., Rahway, NJ, USA (United States)
2019-2025
Wenzhou Medical University
2019-2025
Affiliated Eye Hospital of Wenzhou Medical College
2024
MSD K.K. (Japan)
2024
Beihang University
2024
Tianjin Beichen Hospital
2024
Suzuka University of Medical Science
2022-2023
Weatherford College
2022-2023
Bioprocess Control (Sweden)
2023
Translational Therapeutics (United States)
2014-2019
A simple, high-efficiency, clamshell-style, superhydrophobic plasma separator for point-of-care applications.
Resolvins, maresins, and protectins can be formed from fish oils. These specialized pro-resolving mediators (SPMs) have been implicated in the resolution of inflammation. Synthetic versions such SPMs exert anti-inflammatory effects vitro when administered to animal models. However, their importance as endogenous products sufficient amounts actions vivo remains speculative. We biased our ability detect healthy volunteers by supplementing oil doses shown previously influence blood pressure...
Abstract Chinese hamster ovary (CHO) cells are widely used to produce recombinant proteins, including monoclonal antibodies (mAbs), through various process modes. While fed‐batch (FB) processes have been the standard, a shift toward high‐density perfusion is being driven by increased productivity, flexible facility footprints, and lower costs. Ensuring clearance of process‐related impurities, such as host cell proteins (HCPs), crucial in biologics manufacturing. Although purification remove...
The vertebrate gut microbiota have been implicated in the metabolism of xenobiotic compounds, motivating studies microbe-driven clinically important drugs. Here, we studied interactions between and indomethacin, a nonsteroidal anti-inflammatory drug (NSAID) that inhibits cyclooxygenases (COX) -1 -2. Indomethacin was tested both acute chronic exposure models mice at relevant doses, which suppressed production COX-1- COX-2-derived prostaglandins caused small intestinal (SI) damage. Deep...
Significance Painkillers classified as nonsteroidal antiinflammatory drugs (NSAIDs) are among the most commonly consumed drugs. Although they ameliorate pain effectively by inhibiting enzyme cyclooxygenase, can cause serious cardiovascular complications, including heart attack and stroke. Additionally, NSAIDs have potential to render low-dose aspirin taken reduce risk of stroke ineffective through a drug–drug interaction, there is great uncertainty in how manage patients with disease. We...
Polysorbate is widely used to maintain stability of biotherapeutic proteins in pharmaceutical formulation development. Degradation polysorbate can lead particle formation drug products, which a major quality concern and potential patient risk factor. Enzymatic activity from residual host cell enzymes such as lipases esterases plays role for degradation. Their high activity, often at very low concentration, constitutes analytical challenge the biopharmaceutical industry. In this study, we...
Abstract Host‐cell proteins (HCPs) are the foremost class of process‐related impurities to be controlled and removed in downstream processing steps monoclonal antibody (mAb) manufacturing. However, some HCPs may evade clearance multiple purification reach final drug product, potentially threatening stability patient safety. This study extends prior work on HCP characterization persistence mAb process streams by using mass spectrometry (MS)‐based methods track through for seven mAbs that were...
Abstract Product association of host‐cell proteins (HCPs) to monoclonal antibodies (mAbs) is widely regarded as a mechanism that can enable HCP persistence through multiple purification steps and even into the final drug substance. Discussion this often implies existence or extent directly related strength binding but actual measurements affinity such interactions remain sparse. Two separate avenues investigation HCP‐mAb are reported here. One measurement individual, commonly persistent...
Host cell proteins are process-related impurities in biotherapeutics and can potentially pose risks to patient safety product quality. Specifically, certain host cell-derived enzymes, including lipases, degrade the formulation excipient polysorbate (PS) biopharmaceutical formulations, affecting drug stability liquid formulations. We leveraged multi-omics approaches, transcriptomics, proteomics, activity-based protein profiling (ABPP), identify mechanisms that regulate PS-degradative enzyme...
The success of mRNA-based therapeutics and vaccines is attributed to their rapid development, adaptability, scalable production. Modified ribonucleotides like N1-methylpseudouridine enhance stability reduce immunogenicity but were recently found induce cellular immunity off-target, +1 ribosomal frameshifted protein. We developed a new platform using cell-free translation (CFT) liquid chromatography-tandem mass spectrometry (MS) detect, characterize, quantify antigen proteins from mRNA...
Cell metabolism plays a crucial role in regulating the pluripotency of human embryonic stem cells (hESCs). Chlorogenic acid (CGA), an essential dietary polyphenol, exhibits diverse pharmacological effects on regulation. This study examines CGA cell hESCs using H9 model. At concentration 100 µg/ml, showed low toxicity and had no impact viability cells. Furthermore, it promotes NANOG expression. Importantly, enhances Fatty β-oxidation (FAO), thus promoting proliferation lipid synthesis...
Abnormal thyrotropin receptor (TSHR) signaling drives a spectrum of diseases affecting hundreds millions globally, including Graves' disease (GD), ophthalmopathy (GO), hyperthyroidism, and thyroid cancers. Therefore, TSHR is considered an attractive therapeutic target with immense drug discovery potential. Currently, there are no clinically available inhibitors. To address this unmet medical need, we developed YC3, functional nucleic acid aptamer targeting TSHR, via innovative approach...
To comprehensively identify proteins of the rat liver plasma membrane (PM), we have adopted a proteomics strategy that utilizes sucrose density centrifugation in conjunction with aqueous two-phase partition for isolation, followed by SDS-PAGE, mass spectrometry and bioinformatics. Western blot analysis showed this method results highly purified fractions, which is key to successful proteomics. The PM were separated SDS-PAGE digested trypsin. Through nano-ESI−LC MS/MS identified 428 proteins,...
Cyclooxygenase-2 (COX-2) expression is associated with poor prognosis across a range of human cancers, including breast cancer. The contribution tumor cell-derived COX-2 to tumorigenesis has been examined in numerous studies; however, the role stromal-derived ill-defined. Here, we how myeloid cells, an immune cell subset that includes macrophages, influences mammary progression. In mice engineered selectively lack [myeloid-COX-2 knockout (KO) mice], spontaneous neu oncogene-induced onset was...
Multiple reaction monitoring (MRM) is a liquid chromatography–mass spectrometry (LC–MS) based quantification platform with high sensitivity, specificity, and throughput. It extensively used across the pharmaceutical industry for quantitative analysis of therapeutic molecules. The potential MRM specific host cell proteins (HCPs) in bioprocess, however, has yet to be well established. In this work, we introduce multiplex LC–MRM assay that simultaneously monitors two risk lipases known impact...
Host cell proteins (HCPs) are process-related impurities derived from host organisms, which need to be controlled ensure adequate product quality and safety. In this study, attributes were tracked for several monoclonal antibodies (mAbs) under the intended storage accelerated stability conditions. One attribute not expected indicating is N-glycan heterogeneity profile. However, significant degradation was observed one mAb stressed The root cause instability attributed hexosaminidase B...
Abstract Ribosome biogenesis (RiBi) plays a pivotal role in carcinogenesis by regulating protein translation and stress response. Here, we find that RRP15, nucleolar critical for RiBi checkpoint control, is frequently upregulated primary CRCs higher RRP15 expression positively correlated with TNM stage ( P < 0.0001) poor survival of CRC patients = 0.0011). Functionally, silencing induces ribosome stress, cell cycle arrest, apoptosis, resulting suppression proliferation metastasis....
Abstract The hippocampus is a distinct brain structure that crucial in memory storage and retrieval. To identify comprehensively proteins of hippocampal plasma membrane (PM) detect the neuronal‐specific PM proteins, we performed proteomic analysis rat using following three technical strategies. First, were purified by differential density‐gradient centrifugation from tissue separated one‐dimensional electophoresis, digested with trypsin analyzed electrospray ionization (ESI) quadrupole...
BACKGROUND Microfluidic devices can create hemodynamic conditions for platelet assays. We validated an 8-channel device in a study of interdonor response to acetylsalicylic acid (ASA, aspirin) with whole blood from 28 healthy individuals. METHODS Platelet deposition was assessed before treatment or 24 h after ingestion 325 mg ASA. Whole (plus 100 μmol/L H-d-Phe-Pro-Arg-chloromethylketone inhibit thrombin) further treated ex vivo ASA (0–500 μmol/L) and perfused over fibrillar collagen 300 s...