A5014791053- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal and related cancers
- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Antibiotics Pharmacokinetics and Efficacy
- Lysosomal Storage Disorders Research
- Glioma Diagnosis and Treatment
- Analytical Chemistry and Chromatography
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Estrogen and related hormone effects
- Eicosanoids and Hypertension Pharmacology
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- Testicular diseases and treatments
- Pharmaceutical studies and practices
- Heart Failure Treatment and Management
- Water Treatment and Disinfection
- Pharmaceutical Economics and Policy
- Pneumonia and Respiratory Infections
- Molecular spectroscopy and chirality
- Computational Drug Discovery Methods
- Biosimilars and Bioanalytical Methods
- Geography and Environmental Studies
- Studies on Chitinases and Chitosanases
Centre Hospitalier Universitaire de Rouen
2021-2025
Université de Rouen Normandie
2015-2025
Inserm
2022-2023
Université de Caen Normandie
2023
Normandie Université
2023
University of California, Los Angeles
2023
Institut du Cerveau
2022
Sorbonne Université
2022
Assistance Publique – Hôpitaux de Paris
2022
Institut de Psychiatrie et Neurosciences de Paris
2022
Breaking through the milligram floor When chemists synthesize compounds, threshold for success is at least a of product. This has been true decades—even though biochemical assays have long since descended into microgram territory—and results in part from constraints characterization methods. Buitrago Santanilla et al. present an automated dosing and protocol optimizing chemical reaction conditions on scale. allowed them to screen numerous base ligand combinations catalytic C-N bond-forming...
Metabolic phenotyping is poised as a powerful and promising tool for biomarker discovery in inherited metabolic diseases. However, few studies applied this approach to mcopolysaccharidoses (MPS). Thus, innovative functional may unveil comprehensive impairments MPS biology. This study explores mcopolysaccharidosis VI (MPS VI) or Maroteaux⁻Lamy syndrome (OMIM #253200) which an autosomal recessive lysosomal storage disease caused by the deficiency of arylsulfatase B enzyme. Urine samples were...
Steroids play a key role in numerous physiological processes. Steroid determination is useful tool to explore various endocrine diseases. Because of its specificity, mass spectrometry considered be reference method for the steroids serum compared radioimmunoassay. This technology could progress towards more automation optimal organization clinical laboratories and ultimately benefit patients.
MK-0524 is a potent, selective and orally active Prostaglandin D2 receptor 1 (DP1) antagonist currently under clinical development for the treatment of niacin-induced flushing. Experiments to study pharmacokinetics, metabolism excretion were conducted in rats, dogs monkeys. displayed linear kinetics rapid absorption following an oral dose. Following intravenous (i.v.) administration rats (1 5 mg/kg), mean Clp was ∼2 ∼6 ml/min/kg, T1/2 ∼7 ∼13 h Vdss ∼1 ∼5 L/kg, respectively. In monkeys dosed...
The employment of ethylenediaminetetraacetic acid (EDTA) across several fields in chemistry and biology has required the creation a high number quantitative assays. Nonetheless, determination trace EDTA, especially biologics vaccines, remains challenging. Herein, we introduce an automated high-throughput approach based on EDTA esterification 96-well plates using boron trifluoride-methanol combined with rapid analysis by ultra-high-performance liquid chromatography–triple quadrupole tandem...
(1) Background: Glioblastoma is the most common malignant brain tumor in adults. Its etiology remains unknown cases. pathogenesis consists of a progressive infiltration white matter by tumoral cells leading to neurological deficit, epilepsy, and/or intracranial hypertension. The mean survival between 15 17 months. Given this aggressive prognosis, there an urgent need for better understanding underlying mechanisms glioblastoma unveil new diagnostic strategies and therapeutic targets through...
The pharmacokinetics and metabolism of the l-threo isoleucine thiazolidide dipeptidyl peptidase IV inhibitor, di-[2<i>S</i>,3<i>S</i>]-2-amino-3-methyl-pentanoic-1,3-thiazolidine fumarate (ILT-threo) its allo stereoisomer (ILT-allo) were evaluated in rats, dogs, monkeys. Both compounds well absorbed (>80%) all species, most dose (>60%) was recovered urine. Metabolites identified species included a sulfoxide (M1), sulfone (M2), carbamoyl glucuronide (M3). For both compounds, parent drug...
Both the physiological role of muscarinic receptors for bladder function and therapeutic efficacy antimuscarinic agents overactive syndrome are well documented. We investigated effect with different subtype selectivity on urodynamic parameters in nonhuman primates rodents compared plasma levels these between species. Anesthetized rhesus monkeys were transurethrally catheterized, was infused saline. Urodynamic measured before after intravenous drug administration. Tolterodine (nonselective)...
Abstract Ifosfamide ( IFA ) is a potent alkylating antitumoral agent, but its use limited by neurological side effects. racemic mixture of two enantiomeric forms, R ‐ and S with stereoselective metabolism CYP 3A4 2B6, leading either to bioactive or toxic pathways. In three consecutive cases pediatric patients who exhibited IFA‐induced encephalopathy IIE ), genotyping clinically relevant single‐nucleotide polymorphisms associated decreased 2B6 activities was performed. Genetic investigations...
Nicardipine hydrochloride is an anti-hypertensive drug that used off-label to treat hypertension in children. A previous oral formulation of nicardipine was developed using a commercial vehicle as excipient. However, ready-to-use vehicles are prone supply shortages, and their composition may undergo substantial modifications. The aim this study propose new 2 mg/mL simple excipients. included hydroxypropylmethylcellulose, syrup, polysorbate 80, sodium saccharin, citrate buffer, strawberry...
Abstract Ivermectin is a parasiticide commonly used in humans and livestock. It currently under development for the treatment of pediculosis (head lice) that does not respond to established treatments. A liquid chromatography/turbo ion spray tandem mass spectrometry (LC/TIS‐MS/MS) method determination ivermectin rat human plasma has been developed uses emamectin [4″‐epi‐(methylamino)‐4″‐deoxyavermectin] as internal standard. Sample preparation involved protein precipitation filtration...
Type 2 diabetes (T2D) and hypertension (HTN) are common risk factors of cardiovascular diseases (CVD) characterized by chronic low-grade systemic inflammation impaired endothelial function. This study aimed to assess whether levels non-enzymatic, lipoxygenase (LOX)- cytochrome P450 (CYP)-derived arachidonic acid (ARA) metabolites, which known regulators vascular homeostasis, affected HTN T2D. For this objective, 17 plasma level derivatives ARA were quantitated chromatography coupled with...
Background: Fabry disease (FD) is an X-linked lysosomal due to a deficiency in the activity of α-galactosidase A (GalA), key enzyme glycosphingolipid degradation pathway. FD complex with poor genotype–phenotype correlation. could involve kidney, heart or central nervous system impairment that significantly decreases life expectancy. The advent omics technologies offers possibility global, integrated and systemic approach well-suited for exploration this disease. Materials Methods: Sixty-six...
Children undergoing cancer treatments are at risk for impaired fertility. Cryopreserved prepubertal testicular biopsies could theoretically be later matured in vitro to produce spermatozoa assisted reproductive technology. A complete spermatogenesis has been obtained from mouse tissue, although with low efficiency. Steroid hormones essential the progression of spermatogenesis, aim this study was investigate steroidogenesis and steroid signaling organotypic cultures. Histological, RT-qPCR,...