A5014539337- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microstructure and Mechanical Properties of Steels
- Magnetic Properties of Alloys
- Innovative Microfluidic and Catalytic Techniques Innovation
- Magnetic Properties and Applications
- CO2 Sequestration and Geologic Interactions
- RNA Interference and Gene Delivery
- Structural Load-Bearing Analysis
- Granular flow and fluidized beds
- Peptidase Inhibition and Analysis
- Coal and Coke Industries Research
- Drug Transport and Resistance Mechanisms
- Barrier Structure and Function Studies
- Adenosine and Purinergic Signaling
- Hydrogen embrittlement and corrosion behaviors in metals
- RNA and protein synthesis mechanisms
- Kidney Stones and Urolithiasis Treatments
- Geothermal Energy Systems and Applications
- Nanoparticle-Based Drug Delivery
- High Temperature Alloys and Creep
- Thermochemical Biomass Conversion Processes
- Porphyrin Metabolism and Disorders
- Calcium Carbonate Crystallization and Inhibition
Merck & Co., Inc., Rahway, NJ, USA (United States)
2020-2021
Rensselaer Polytechnic Institute
2011-2019
Pennsylvania State University
2011-2012
Northwestern University
1971
Polysorbate is widely used to maintain stability of biotherapeutic proteins in pharmaceutical formulation development. Degradation polysorbate can lead particle formation drug products, which a major quality concern and potential patient risk factor. Enzymatic activity from residual host cell enzymes such as lipases esterases plays role for degradation. Their high activity, often at very low concentration, constitutes analytical challenge the biopharmaceutical industry. In this study, we...
Abstract Mössbauer spectra of alloys in the range Fe–24 at% Cr to Fe–60 have been analyzed using an extension high concentrations results for dilute alloys. The decrease hyperfine field at Fe nucleus due presence one near or next neighbor was found be 7.5%, concentration independent over studied, and excellent accord with value 7.6% Fe. isomer shift per −0.02 mm/s, also good agreement −0.03 mm/s same parameters gave equally satisfactory fits randomly polarized absorbers experiments absorber...
Abstract Biopharmaceutical product and process development do not yet take advantage of predictive computational modeling to nearly the degree seen in industries based on smaller molecules. To assess advance progress this area, spirited coopetition (mutually beneficial collaboration between competitors) was successfully used motivate industrial scientists develop, share, compare data methods which would normally have remained confidential. The first “Highland Games” competition held...
Abstract Background In this study, we have demonstrated the design, screening and selection of peptide ligands for affinity capture human growth hormone (hGH) from yeast cell cultures. Results Ligand design was carried out using multiple approaches based on primary sequence structures natural binding partners hGH. Screening potential peptides conducted high throughput microarray platforms followed by assessment in‐solution to hGH fluorescence polarization. Peptide leads were subsequently...
Reviewing the uses of accelerated curing concrete, this article from Indian Central Building Research Institute at Roorkee notes that various forms have been used, but mostly for precast work. Attention has now focused on rapid in situ construction, and paper presents results direct electric curing, which proved to be more economical consume less energy than other methods. An appendix outlines finite element method formulation used decide number electrodes required their distribution pattern...
We explore the relationship between mechanical, transport, and critical state characteristics of coal–biomass mixtures by evaluating mixture composition, stress, strength, rhelogy, permeability mixtures. report measurements strength evolution for uniformly graded (passing no. 200 mesh) granular in proportions (a) 100% sub-bituminous coal, (b) 75–25% coal–biomass, (c) biomass. observe response at confining stresses range 5 25 MPa strain rates ∼10–4/s. The pure biomass is most compliant...
We describe a general strategy for the design and discovery of affinity peptides protein from its natural ligands. Our approach is guided by protein-protein interactions in systems focuses on hetero-trimeric complex cardiac troponin I (cTnI), C (cTnC) T (cTnT). A key premise this work that cTnC cTnT, owing to their innate ability bind cTnI, are potential templates cTnI-binding peptides. Relying only knowledge primary sequences we designed library short overlapping span entirety cTnT tested...
We explore the feasibility of combining circulation supercritical CO2 in EGS reservoirs with integrated gasification combined cycle (IGCC) power generation. The symbiotic benefits this pairing increase net output over use either system isolation, reduces water per MWe, and substantially fugitive emissions conventional thermal plants. A prototypical plant set arid southwestern U.S. (environs Albuquerque, NM) could sustain anticipated rates 800–1600 kg/s a 200 °C reservoir, resulting ∼150–300...
Drug-delivery to the brain is a significant challenge due presence of formidable blood-brain barrier (BBB). Current therapeutic interventions treat disorders include surgical implants or catheters, both which are highly invasive and carry risk long term neurological damage. In contrast, non-invasive strategies based on endogenous transport pathways in attractive alternatives for drug delivery brain. We focusing specific pathway called receptor-mediated transcytosis (RMT) that involves...
Abstract Polysorbate is widely used to maintain stability of biotherapeutic proteins in formulation development. Degradation polysorbate can lead particle formation drug products, which a major quality concern and potential patient risk factor. Enzymatic activity from residual enzymes such as lipases esterases cause degradation. Their high activity, even at low concentration, constitutes analytical challenge. In this study, we evaluated optimized the activity-based protein profiling (ABPP)...