A5053221838- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Microfluidic and Capillary Electrophoresis Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Innovative Microfluidic and Catalytic Techniques Innovation
- Protein purification and stability
- Inhalation and Respiratory Drug Delivery
- Advanced Data Storage Technologies
- Semiconductor materials and devices
- Indoor Air Quality and Microbial Exposure
- Advanced Sensor and Energy Harvesting Materials
- Platelet Disorders and Treatments
- Odor and Emission Control Technologies
- 3D IC and TSV technologies
- Molecular Communication and Nanonetworks
- Monoclonal and Polyclonal Antibodies Research
- Medical Research and Treatments
- Surface Modification and Superhydrophobicity
- Spaceflight effects on biology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Automata and Applications
- Advanced Biosensing Techniques and Applications
- Electrohydrodynamics and Fluid Dynamics
- Electrowetting and Microfluidic Technologies
- Spectroscopy and Chemometric Analyses
Sartorius (United States)
2022-2024
Seoul National University
2012-2024
Massachusetts Institute of Technology
2017-2023
Samsung (South Korea)
2019-2021
Vassar College
2021
Government of the Republic of Korea
2017
UPMC Montefiore
2017
University of Pittsburgh
2017
Korea Advanced Institute of Science and Technology
2011
We present a sugar-templated polydimethylsiloxane (PDMS) sponge for the selective absorption of oil from water. The process fabricating PDMS does not require any intricate synthesis processes or equipment and it is environmentally hazardous, thus promoting potential in environmental applications. proposed can be elastically deformed into shape, compressed repeatedly air liquids without collapsing. Therefore, absorbed oils organic solvents readily removed reused by simply squeezing sponge,...
Abstract Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires reliable and efficient cell retention device that also maintains viability. Current filtration methods, such as tangential flow using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability productivity issues low viability, product retention, an increased contamination risk associated with filter replacement. We introduce novel based on inertial sorting...
Assessment of airway secretion cells, both for research and clinical purposes, is a highly desired goal in patients with acute chronic pulmonary diseases. However, lack proper cell isolation enrichment techniques hinder downstream evaluation characterization cells found secretions. Here, we demonstrate novel method to capture immune-related from secretions using closed-loop separation spiral inertial microfluidics (C-sep). By recirculating the output focusing stream back input reservoir...
Data storage is one of the hottest discussion topics in today's connected world. The amount data growth expected to be exponential, while budget and space remain constricted. Since transformation device from planar NAND 3D V-NAND [1], areal density semiconductor devices has continuously evolved surpassed magnetic hard drives. By providing largest capacity smallest footprint, been leading center revolution recent years. However, 3D-technology scaling faces several technical challenges [2]....
Removing nonviable cells from a cell suspension is crucial in biotechnology and biomanufacturing. Label-free microfluidic separation devices based on dielectrophoresis, acoustophoresis, deterministic lateral displacement are used to remove cells. However, their volumetric throughputs test concentrations generally too low be useful typical bioreactors In this study, we demonstrate the efficient removal of small (<10 μm) while maintaining viable using inertial sorting characterize performance....
High-throughput isolation of<italic>C. elegans</italic>eggs from a mixed worm population in spiral chip using inertial microfluidics.
Inertial microfluidics has enabled many impactful high throughput applications. However, devices fabricated in soft elastomer (i.e., polydimethylsiloxane (PDMS)) suffer reliability issues due to significant deformation generated by the pressure and flow rates inertial microfluidics. In this paper, we demonstrated deformation-free mass-producible plastic spiral microfluidic for high-throughput cell separation The design of deformable PDMS was translated their version compensating channel...
The exponential data size growth in high-speed networks is a key motivator for nonvolatile memory development. To support this demand, higher density NAND required: with smaller cell and interface speed. Generally, scaling down technology requires addressing several common issues: 1) As the number of WL stack layers increases, cell-string current reduced due to increased resistance string, 2) Deterioration cell-to-cell interference, reduction pitch, 3) Support IO bandwidth faster transfer...
Although droplet microfludics is a promising technology for handling number of liquids single type analyte, it has limitations in thousands different types analytes multiplex assay. Here, we present novel "liquid-capped encoded microcapsule", which applicable to various liquid format assays. Various drops can be graphically and arrayed without repeated dispensing processes, evaporation, the risk cross-contamination. Millions nanoliter-scale are encapsulated within microcapsules...
Abstract Medium perfusion is critical in maintaining high cell concentration cultures. The conventional membrane filtration method for medium exchange has been challenged by the fouling and clogging of filters long‐term In this study, we present a miniature auto‐perfusion system that can be operated inside common‐size laboratory incubator. equipped with spiral microfluidic chip retention to replace filters, which fundamentally overcomes problem. We showed supported continuous culture Chinese...
We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size quantity directly from the culture supernatant during high-cell-concentration CHO cell perfusion culture. A microfluidic device enables clog-free retention bench-scale (350 mL) bioreactor that continuously produces containing monoclonal antibodies (IgG1). nanofluidic monitors in supernatant. The continuous-flow fully automated operation this analytics reduces design complexity offers...
Abstract Separation of high‐density suspension particles at high throughput is crucial for many chemical, biomedical, and environmental applications. In this study, elasto‐inertial microfluidics used to manipulate ultra‐high‐density cells achieve stable equilibrium positions in microchannels, aided by the inherent viscoelasticity cell suspension. It demonstrated that Chinese hamster ovary (>26 packed volume% (PCV%), >95 million mL −1 ) can be focused distinct lateral under...
Abstract Chinese hamster ovary (CHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins, such as monoclonal antibodies. Enhancement productivity these CHO is one top priorities in biopharmaceutical industry to reduce manufacturing cost. Although there are many different methods (e.g. temperature, pH, feed) improve protein cells, role physiologically relevant hydrostatic pressure cell culture has not reported yet. In this study,...
Bioprocess optimization towards higher productivity and better quality control relies on real-time process monitoring tools to measure culture parameters. Cell concentration viability are among the most important parameters be monitored during bioreactor operations that typically determined using optical methods an extracted sample. In this paper, we have developed online non-invasive sensor cell based Doppler ultrasound. An ultrasound transducer is mounted outside vessel emits a high...
Online monitoring of monoclonal antibody product titers throughout biologics process development and production enables rapid bioprocess decision-making optimization. Conventional analytical methods, including high-performance liquid chromatography turbidimetry, typically require interfacing with an automated sampling system capable online fractionation, which suffers from increased cost, a higher risk failure, mechanical complexity the system. In this study, novel nanofluidic for continuous...
Consumer spray products (CSPs) are widely used in daily life, yet it is challenging to find that fully disclose all components posing health risks. Existing studies primarily focus on product or VOC quantities emitted during use. Therefore, this study aimed measure the concentrations by CSPs at varying distances. 47 available Korean market were selected, spanning three groups: antiseptics/insecticides (11), aromatic deodorants (16), and coating/polishing agents (20). air samples collected...
We introduce a novel platform for cell line optimization mAb production using digital microfluidics. The enables testing of culture samples in 6–8 μL droplets with semi-automated viability, media pH, and antibody assays.
Airway secretions contain a large number of immune-related cells, e.g., neutrophils, macrophages, and lymphocytes, which can be used as major resource to evaluate variety pulmonary diseases, both for research clinical purposes.However, due the heterogeneous viscous nature patient mucus, there is currently no reliable dissociation method that does not damage host immune cells in airway secretion.In this research, we introduce sample preparation uses inertial microfluidics patient's...
In this paper, we demonstrated dispensing of thousands heterogeneous liquid drops into microwells with only a single pipetting, which would normally requires individual pipetting. Key idea is first to fabricate capped Teflon microshells fluorescent encoding and then self-assemble those encoded particles microwells. Capped liquids are released by breaking the microshell pulsed laser. We believe process enable ultraplex microarray phase assay capability, whereas current multiplexed technology...
Suspension cultures of Chinese hamster ovary (CHO) cells are extensively employed in biopharmaceutical production. During cultivation, CHO cell clusters often form due to death and other irregularities, substantially reducing growth productivity. In this study, we introduce a novel method for the selective removal large from bioreactors, utilizing large-particle trapping effect spiral inertial microfluidics. A single microfluidic device effectively removed culture, demonstrating high...