A5071804019- Atherosclerosis and Cardiovascular Diseases
- Antioxidant Activity and Oxidative Stress
- Cholesterol and Lipid Metabolism
- Advanced Glycation End Products research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Lupus Erythematosus Research
- Nitric Oxide and Endothelin Effects
- Neurological Disease Mechanisms and Treatments
- Parathyroid Disorders and Treatments
- Hepatitis C virus research
- Extracellular vesicles in disease
- Immune Response and Inflammation
- Alzheimer's disease research and treatments
- Bone and Dental Protein Studies
- MicroRNA in disease regulation
- Paraoxonase enzyme and polymorphisms
- Chemokine receptors and signaling
- Eicosanoids and Hypertension Pharmacology
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Galectins and Cancer Biology
- Acute Kidney Injury Research
- Sphingolipid Metabolism and Signaling
- Apelin-related biomedical research
- Cholinesterase and Neurodegenerative Diseases
Centre Hospitalier Universitaire Amiens-Picardie
2014-2025
Université de Picardie Jules Verne
2016-2025
Mécanismes physiopathologiques et conséquences des calcifications cardiovasculaires
2018-2025
Physiopathologie et Epidémiologie des Maladies Respiratoires
2018-2022
Laboratoire de Biochimie
2015-2018
Inserm
2008-2018
Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources
2017
Biologie des Plantes et Innovation
2017
University of California, San Diego
2000-2011
Hôpital Bichat-Claude-Bernard
2011
Innate immune responses to oxidized low-density lipoprotein LDL (LDL) regulate the development of atherosclerosis. We demonstrated previously that an early form LDL, minimally modified (mmLDL), triggers cytoskeletal rearrangements in macrophages via CD14 and Toll-like receptor 4 (TLR4)/MD-2. Because lipopolysaccharide (LPS) activation TLR4 leads proinflammatory gene expression, this study, we asked whether mmLDL also induced signaling.We studied cytokine secretion signaling J774 primary...
Toll-like receptor (TLR)4 recognizes microbial pathogens, such as lipopolysaccharide, and mediates lipopolysaccharide-induced proinflammatory cytokine secretion, well uptake by macrophages. In addition to exogenous TLR4 modified self, minimally oxidized low-density lipoprotein (mmLDL). Here we report that mmLDL its active components, cholesteryl ester hydroperoxides, induce TLR4-dependent fluid phase typical of macropinocytosis. We show induced recruitment spleen tyrosine kinase (Syk) a...
There is growing evidence that CD36 has an important physiological function in the uptake of oxidized low density lipoprotein (OxLDL) by macrophages. However, ligand specificity and nature ligands on OxLDL mediate binding to remain ill defined. Results from recent studies suggested some macrophage scavenger receptors involved recognized <i>both</i> lipid protein moieties OxLDL, but there was no conclusive direct for this. The present were undertaken test whether a single, well characterized...
The CCR2-mediated recruitment of monocytes into the vessel wall plays an important role in all stages atherosclerosis. In recent studies, we have shown that lipoproteins can modulate CCR2 expression and identified native LDL as a positive regulator. contrast, oxidized (OxLDL), which is mainly formed aortic intima, reduces expression, promotes monocyte retention, may cause pathological accumulation wall. We now provide evidence OxLDL by activating intracellular signaling pathways involve...
Scavenger receptor class B type I (SR-BI) has been established as the primary mediator of selective transfer lipids from HDL to mammalian cells. In addition its role in cholesterol metabolism, SR-BI shown bind apoptotic cells and thus could theory also function a scavenger receptor. We now show that binds oxidized LDL (OxLDL) with high affinity (Kd 4.0 ± 0.5 μg/ml) mediates internalization degradation an extent comparable other receptors, when normalized binding activity. The best...
Based on their role in T-cell homing into nonlymphoid tissue, we examined the of homeostatic chemokines CCL19 and CCL21 common receptor CCR7 coronary artery disease (CAD).We performed studies patients with stable (n=40) unstable angina healthy controls (n=20), vitro T-cells macrophages, apolipoprotein-E-deficient (ApoE-/-) mice human atherosclerotic carotid plaques. We found increased levels within lesions ApoE-/- mice, plaques, plasma CAD patients. Whereas strong expression was seen from...
Cell entry of hepatitis C virus, pseudoparticles (HCVpp) and cell culture grown virus (HCVcc), requires the interaction viral glycoproteins with CD81 other as yet unknown cellular factors. One these is likely to be scavenger receptor class B type I (SR-BI). To further understand role SR-BI, we examined effect SR-BI ligands on HCVpp HCVcc infectivity. Oxidized low-density lipoprotein (oxLDL), but not native LDL, potently inhibited entry. Pseudoparticles bearing unrelated or bovine diarrhea...
Recent data derived primarily from studies in animal models suggest that fractalkine (CX3CL1) and its cognate receptor, CX3CR1, play a role atherogenesis. We, therefore, hypothesized enhanced CX3CL1/CX3CR1 expression may promote atherogenesis patients with coronary artery disease (CAD).We examined the plasma levels of CX3CL1 CX3CR1 peripheral blood mononuclear cells (PBMC) various CAD populations (30 previous myocardial infarction, 40 stable angina, unstable total 35 controls) used...
We have previously shown that CD36 recognizes oxidation products of phospholipids on oxidized LDL (OxLDL) such as 1-palmitoyl-2-(5'-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC). The current study was designed to examine whether the phosphocholine (PC) headgroup in POVPC constitutes an obligatory binding target for CD36. To contribution PC CD36, we used well-defined synthetic (OxPLs) cross-linked BSA or a hexapeptide. OxPL adducts were then tested their ability bind CD36-transfected cells...
Transglutaminase 2 (TG2), a broadly expressed regulator of protein cross-linking, wound healing, and tissue fibrosis, mediates apoptotic cell ingestion transforming growth factor-beta release by macrophages thereby can limit leukocyte-mediated inflammation. In atherosclerosis, oxidative stress accumulation unesterified cholesterol stimulate atherosclerotic lesion apoptosis. Cell death in advanced lesions promotes expansion vulnerable plaques prone to rupture. Hence, we tested the hypothesis...
Objective— Lipid-loaded macrophage-derived foam cells populate atherosclerotic lesions and produce many pro-inflammatory plaque-destabilizing factors. An excessive accumulation of extensively oxidized low-density lipoprotein (OxLDL) or free cholesterol (FC), both which are believed to be major lipid components macrophages in advanced lesions, rapidly induces apoptosis macrophages. Indeed, there is evidence macrophage death but how the surviving avoid induced by OxLDL, FC, other factors not...
Background: Vascular calcification (VC) is a cardiovascular complication associated with high mortality rate among patients diseases such as atherosclerosis and chronic kidney disease. During VC, vascular smooth muscle cells (VSMCs) undergo an osteogenic switch secrete heterogeneous population of extracellular vesicles (EVs). Recent studies have shown involvement EVs in the inflammation oxidative stress observed VC. We aimed to decipher role mechanism action macrophage-derived propagation on...
Endothelial dysfunction plays a key role in stroke chronic kidney disease patients. To explore the underlying mechanisms, we evaluated effects of two uremic toxins on cerebral endothelium function. bEnd.3 cells were exposed to indoxyl sulfate (IS) and inorganic phosphate (Pi). Nitric oxide (NO), reactive oxygen species (ROS) O2•– measured using specific fluorophores. Peroxynitrite eNOS uncoupling ebselen, peroxide scavenger, tetrahydrobiopterin (BH4), respectively. Cell viability decreased...
Background/Objectives: Delays in bone healing and complications of remodeling constitute a major medical problem—particularly older adults patients with comorbidities. Current therapeutic approaches are based on strategies that promote regeneration. We recently identified disaccharide compound (DP2) enhances vitro mineralization human osteoblast cells via the early activation Runx2 induction differentiation. Methods: First, calcium quantification assay was performed to assess MC3T3-E1 cells....
Endothelial dysfunction in uremia can result cell-to-cell junction loss and increased permeability, contributing to cardiovascular diseases (CVD) development. This study evaluated the impact of uremic milieu on endothelial morphology cell junction’s proteins. We (i) serum levels inflammatory biomarkers a cohort chronic kidney disease (CKD) patients expression VE-cadherin Zonula Occludens-1 (ZO-1) proteins cells (ECs) arteries removed from CKD during renal transplant; (ii) ECs vitro under...
Systematic screening for congenital hypothyroidism by heel-stick sampling has revealed unexpected heterogeneity in the geographic distribution of newborn thyroid-stimulating hormone concentrations Picardy, France. We explored a possible relationship with environmental pollutants.Zip code geolocation data from mothers newborns without born 2021 were linked to ecological set airborne (particulate matter diameter 2.5 μm or less [PM2.5] 10 [PM10]) and tap-water (nitrate perchlorate ions...