A5069725659- Dialysis and Renal Disease Management
- Lysosomal Storage Disorders Research
- Extracellular vesicles in disease
- Metabolomics and Mass Spectrometry Studies
- Heme Oxygenase-1 and Carbon Monoxide
- MicroRNA in disease regulation
- Acute Kidney Injury Research
- Erythropoietin and Anemia Treatment
- Pregnancy and preeclampsia studies
- Atherosclerosis and Cardiovascular Diseases
- Clinical Nutrition and Gastroenterology
- Metabolism and Genetic Disorders
- COVID-19 Clinical Research Studies
- Education Pedagogy and Practices
- Pancreatitis Pathology and Treatment
- Pharmacological Effects and Toxicity Studies
- Drug-Induced Ocular Toxicity
- Ion Transport and Channel Regulation
- Advanced Glycation End Products research
- Piperaceae Chemical and Biological Studies
- Hemoglobinopathies and Related Disorders
- Polymer Surface Interaction Studies
- Phytochemistry Medicinal Plant Applications
- bioluminescence and chemiluminescence research
- Cerebrovascular and genetic disorders
Universidade Federal do Paraná
2016-2025
Hospital de Clínicas Universidade Federal do Paraná
2024
Universidade de Uberaba
2024
Universidade Federal do Triângulo Mineiro
2024
Biointerface decoration with ligands is a crucial requirement to modulate biodistribution, increase half-life, and provide navigation control for targeted micro- or nanostructured systems. To better the process of ligand functionalization over three-dimensional (3D) polyester surfaces, we report characterization hybrid proteins developed enhance anchoring efficiency polymeric surfaces preserve optimal spatial orientation: sfGFP, mRFP1, RBD were attached substrate binding domain (SBD) formed...
Endothelial dysfunction in uremia can result cell-to-cell junction loss and increased permeability, contributing to cardiovascular diseases (CVD) development. This study evaluated the impact of uremic milieu on endothelial morphology cell junction’s proteins. We (i) serum levels inflammatory biomarkers a cohort chronic kidney disease (CKD) patients expression VE-cadherin Zonula Occludens-1 (ZO-1) proteins cells (ECs) arteries removed from CKD during renal transplant; (ii) ECs vitro under...
Advanced glycation end products (AGEs) have been related to the pathogenesis of cardiovascular diseases (CVD), chronic kidney disease (CKD) and diabetes mellitus. We sought investigate binding capacity sevelamer both AGEs uremic serum in vitro then test this pharmaceutical effect as a potential vascular anti-inflammatory strategy.AGEs were prepared by albumin characterized absorbance electrophoresis. Human endothelial cells incubated culture media containing with or without sevelamer....
Organic anion transporters (OATs) are involved in the uptake of uremic toxins such as p-cresyl sulfate (PCS) and indoxyl (IS), which play a role endothelial dysfunction patients with chronic kidney diseases (CKD). In this study, we investigated OAT1 OAT3 PCS IS into human cells. was synthesized via <i>p</i>-cresol sulfation characterized using analytical methods. The cells were treated absence presence probenecid (Pb), an OAT inhibitor. Cell viability assessed MTT assay. absorbed...
Abstract Epigenetic analysis is a fundamental part of understanding pathophysiological processes with potential applications in diagnosis, prognosis, and assessment disease susceptibility. changes have been widely studied chronic obstructive pulmonary (COPD), but currently, there no molecular marker used to improve the treatment patients. Furthermore, this progressive risk factor for development more severe COVID-19. Methylation-specific polymerase chain reaction (MSP-PCR) plays an important...
<b><i>Background and Aims:</i></b> Inflammation endothelial damage play a pivotal role in Fabry disease (FD) manifestations. In daily clinical practice, FD is mainly monitored by traditional biomarkers of target organ injury, such as serum creatinine proteinuria, which provide no information about inflammation damage. <b><i>Materials Methods:</i></b> We investigated the levels 3-nitrotyrosine (3-NT), an oxidative stress biomarker, growth...
In vitro anti-inflammatory effects of vitamin D supplementation may be blurred in hemodialysis patients Paulo C.
Resumo: A formação inicial de professores inclui o estágio em docência, que proporciona a vivência do cotidiano escolar. partir disso, presente estudo investiga experiência quatro estagiários Biologia diferentes turmas Ensino Médio da Educação Básica. Para tanto, cada estagiário produziu relatos foram submetidos à análise ação docente utilizando instrumento Matriz 3x3 baseado no sistema didático Chevallard e na gestão das relações com saber Charlot, proposto por Arruda, Lima Passos. O foi...
Piper amalago L. is a medicinal plant traditionally used as healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of aqueous crude extract obtained from P. leaves, in different concentrations incubation times, using vitro model mouse fibroblasts (3T3). was tested at 24 h time analysis cell viability, cytotoxicity, proliferation, morphology, immunostaining, adhesion spreading assays, well determine hydroxyproline...
Aim: identify the main aspects of Fabry disease (FD) from clinical suspicion to diagnosis, its vascular and cellular effects, in addition treatment. Methods: This study is an integrative review literature supported by search for articles databases, Pub Med, Lilacs, Scielo, journals, websites, with following descriptors “Fabry disease”, Disease treatment”, “Agalsidase-β”, “Agalsidase-α”, “Migalatast”, “inflammatory biomarkers” “oxidative stress”, using original research reviews between years...
Abstract Background and Aims Fabry Disease (FD) is a rare disease characterized by mutation in the GLA gene consequently functional anomaly of α-Galactosidase A (α-Gal) enzyme. The lack this enzyme leads to lysosomal accumulation glycosphingolipids, mainly globotriaosylceramide (Gb3), different cell types, causing several symptoms throughout body, which aggravate during patient's longevity. We already demonstrated that exposure endothelial cells chloroquine, mimic FD effects, human cells. In...
Abstract Background and Aims Endothelial dysfunction caused by the accumulation of uremic toxins is an important contributor to development cardiovascular disease in patients with chronic kidney (CKD). The toxin trimethylamine n-oxide (TMAO) associated oxidative stress, vascular calcification, inflammation, triggering release several proinflammatory molecules Extracellular Vesicles (EVs). Clinical studies have shown that CKD high levels circulating EVs, however, their role still poorly...