A5064624650- Protein purification and stability
- Venomous Animal Envenomation and Studies
- melanin and skin pigmentation
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Viral Infectious Diseases and Gene Expression in Insects
- Antioxidant Activity and Oxidative Stress
- Marine Invertebrate Physiology and Ecology
- Ion Channels and Receptors
- Invertebrate Immune Response Mechanisms
- Insect and Pesticide Research
- Blood disorders and treatments
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Zebrafish Biomedical Research Applications
- Blood groups and transfusion
- Platelet Disorders and Treatments
- Calpain Protease Function and Regulation
- Polymer Surface Interaction Studies
- Vestibular and auditory disorders
- biodegradable polymer synthesis and properties
- Glycosylation and Glycoproteins Research
- Nicotinic Acetylcholine Receptors Study
Universidade Federal do Paraná
2020-2025
Departamento de Ciência e Tecnologia
2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2023
Université de Tours
2020-2022
Abstract Platelet glycoprotein VI (GPVI) is attracting interest as a potential target for the development of new antiplatelet molecules with low bleeding risk. GPVI binding to vascular collagen initiates thrombus formation and interactions fibrin promote growth stability thrombus. In this study, we show that glenzocimab, clinical stage humanized antibody fragment (Fab) high affinity GPVI, blocks both ligands through combination steric hindrance structural change. A cocrystal glenzocimab an...
Biointerface decoration with ligands is a crucial requirement to modulate biodistribution, increase half-life, and provide navigation control for targeted micro- or nanostructured systems. To better the process of ligand functionalization over three-dimensional (3D) polyester surfaces, we report characterization hybrid proteins developed enhance anchoring efficiency polymeric surfaces preserve optimal spatial orientation: sfGFP, mRFP1, RBD were attached substrate binding domain (SBD) formed...
Bites by spiders from Loxosceles genus often lead to a wide variance in envenomation profile of patients and diagnosis is difficult due the number diseases that mimic loxoscelism. In such context, it interest consider design standardized recombinant colorimetric antibodies for specific detection individual circulating toxins biological fluids envenomed patients. We have previously prepared monoclonal murine IgG (LiMab7) reacts with intermedia venom components 32-35kDa neutralizes...
Envenoming due to Loxosceles spider bites still remains a neglected disease of particular medical concern in the Americas. To date, there is no consensus for treatment envenomed patients, yet horse polyclonal antivenoms are usually infused patients with identified severe conditions. It widely known that venom proteins 30–35 kDa range sphingomyelinase D (SMasesD) activity, reproduce most toxic effects observed loxoscelism. Hence, we believe monoclonal antibody fragments targeting such toxins...
In order to increase the successful development of recombinant antibodies and fragments, it seems fundamental enhance their expression and/or biophysical properties, such as thermal, chemical, pH stabilities. this study, we employed a method bases on replacing antibody framework region sequences, in promote more particularly single-chain Fragment variable (scFv) product quality. We provide evidence that mutations VH- C-C' loop might significantly improve prokaryote production well-folded...
The zebrafish is an animal model of increasing use in many biomedical fields study, including toxicology, inflammation, and tissue regeneration. In this paper, we have investigated the inflammatory effects Loxosceles intermedia's venom (LIV) on zebrafish, as well Maresin 2 (Mar2) Resolvin D5 (RvD5), two specialized pro-resolving mediators (SPMs), context regeneration after fin fold amputation. Furthermore, concentrations LIV (250-2000 ng) were assayed for their haemolytic vitro, and,...