A5056486242- Nanoparticle-Based Drug Delivery
- Dendrimers and Hyperbranched Polymers
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Nanoplatforms for cancer theranostics
- Proteoglycans and glycosaminoglycans research
- Advanced Drug Delivery Systems
- DNA Repair Mechanisms
- Bone health and treatments
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Drug Solubulity and Delivery Systems
- Metal-Organic Frameworks: Synthesis and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer Research and Treatment
- Bacteriophages and microbial interactions
- Graphene and Nanomaterials Applications
- RNA Research and Splicing
- Corneal Surgery and Treatments
Université Paris-Saclay
2016-2024
Institut de Biologie Intégrative de la Cellule
2021-2024
Centre National de la Recherche Scientifique
2011-2023
CEA Paris-Saclay
2021-2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021-2023
Institut Galien Paris-Saclay
2013-2022
Université Paris-Sud
2013-2019
Inserm
2019
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2015-2018
Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique
2015
Abstract Nanoparticles made of metal-organic frameworks (nanoMOFs) attract a growing interest in gas storage, separation, catalysis, sensing and more recently, biomedicine. Achieving stable, versatile coatings on highly porous nanoMOFs without altering their ability to adsorb molecules represents today major challenge. Here we bring the proof concept that outer surface can be specifically functionalized rapid, biofriendly non-covalent manner, leading stable coatings. Cyclodextrin bearing...
A versatile and efficient functionalization strategy for polymeric nanoparticles (NPs) has been reported successfully applied to PEGylated, biodegradable poly(alkyl cyanoacrylate) (PACA) nanocarriers. The relevance of this platform was demonstrated in both the fields cancer Alzheimer's disease (AD). Prepared by copper-catalyzed azide–alkyne cycloaddition (CuAAC) subsequent self-assembly aqueous solution amphiphilic copolymers, resulting functionalized NPs exhibited requisite characteristics...
Inflammation is an essential component of many lung diseases, including asthma, chronic obstructive pulmonary disease (COPD), or acute injury. Our purpose was to design efficient carriers for delivery small interfering RNA (siRNA) targeting tumor necrosis factor (TNF-α) in injury model. To achieve this goal, two different types phosphorus-based dendrimers with either pyrrolidinium morpholinium as terminal protonated amino groups were selected their better biocompatibility compared other...
Cancer stem cells (CSCs) are a subset of tumor that has the ability to self-renew and generate diverse comprise mass. The cell-surface glycoprotein CD44 is one most common surface markers used identify CSCs. Aptamers synthetic oligonucleotides selected from pools random sequences can bind wide range targets with high affinity specificity. In this study, systematic evolution ligands by exponential enrichment (SELEX) technology was isolate RNA aptamers using human recombinant full-length...
Multifunctional poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) nanoparticles for cancer cell targeting and imaging have been designed by a combination of ring-opening polymerization "click" chemistry. Nanoparticles containing both ligand fluorescent probe were prepared blending PLA-b-PEG–ligand, PLA-b-PEG–fluorescent probe, PLA-b-PEG–OMe copolymers at the molar ratios necessary to achieve desired surface densities. This strategy has illustrated preparation large library variety...
The nucleoprotein (NP) of influenza A virus (IAV) required for IAV replication is a promising target new antivirals. We previously identified by in silico screening naproxen being dual inhibitor NP and cyclooxygenase COX2, thus combining antiviral anti-inflammatory effects. However, the recently shown strong COX2 potential makes inhibition undesirable. Here we designed synthesized two series analogues called derivatives 2, 3, 4 targeting highly conserved residues RNA binding groove,...
Respiratory syncytial virus (RSV), the most common cause of bronchiolitis and pneumonia in infants, elicits a remarkably weak innate immune response. This is partly due to type I interferon (IFN-I) antagonism non-structural RSV NS1 protein. It was recently suggested that could modulate host transcription via an interaction with MED25 subunit Mediator complex. Previous work emphasized role C-terminal helix α3 for recruitment ACID domain, target factors (TFs). Here we show α/β core domain...
The dynamics of the formation siRNA-lipoplexes coated with hyaluronic acid (HA) and parameters influencing their supramolecular organization were studied. insertion a HA-dioleylphosphatidylethanolamine (DOPE) conjugate in liposome structure as well subsequent complexation siRNA increased size. Lipoplexes around 110 nm at high ± charge ratios zeta potential +50 mV 230 low ratios, that decreased to negative values, reaching −45 mV. addition did not compromise binding liposomes, although these...
We report herein a detailed study concerning the impact of different bioconjugation and nanoformulation strategies on in vitro targeting ability peptide-decorated squalenoyl gemcitabine (SQdFdC) nanoparticles (NPs). NPs have been functionalized with CKAAKN peptide, previously identified as an efficient homing device within pancreatic pathological microenvironment. Two approaches followed: (i) either peptide was directly conjugated at surface preformed SQdFdC (conjugation after NP formation)...
In the field of dendrimers targeting small interfering RNA (siRNA) delivery, dendrimer structural properties, such as surface chemistry, play a crucial role in efficiency complexation.
During the initiation step of bacterial genome replication, replicative helicases depend on specialized proteins for their loading onto oriC . DnaC and DnaI were first loaders to be characterized. However, most bacteria do not contain any these genes, which are domesticated phage elements that have replaced ancestral unrelated loader gene dciA several times during evolution. To understand how DciA assists DnaB, crystal structure complex from Vibrio cholerae was determined, in two Vc...
DciA is the ancestral bacterial replicative helicase loader, punctually replaced during evolution by DnaC/I loaders of phage origin. DnaC helps to load onto DNA cracking open hexameric ring, but mechanism loading remains unknown. We demonstrate electron microscopy, nuclear magnetic resonance (NMR) spectroscopy, and biochemistry experiments that DciA, which folds into a KH-like domain, interacts with not only single-stranded also double-stranded DNA, in an atypical mode. Some point mutations...
Loxosceles spider bites often lead to serious envenomings and no definite therapy has yet been established. In such a context, it is of interest consider an antibody-based targeted therapy. We have previously prepared murine monoclonal IgG (LiMab7) that binds 32–35 kDa components intermedia venom neutralizes the dermonecrotic activity venom. Here, we re-engineered LiMab7 into recombinant diabody. The protein was produced in bacteria then functionally characterized. It proved be efficient at...
The structure of the ribonucleoprotein complexes is crucial to viral transcription and replication influenza virus, but association nucleoprotein (NP) with polymerase remains be characterized at molecular level. Here, we identify a peptide acidic subunit PA(1-27) that associates NP. Docking dynamics simulations suggest similar NP binding site PA(1-186). PA(1-27)-NP complex by surface plasmon resonance fluorescence using recombinant proteins pull-down assays in infected cells. may have role...
We have designed a new Bodipy scaffold for efficient in vivo photoacoustic (PA) imaging of nanoparticles commonly used as drug nanovectors. The dye has an optimized absorption band the near-infrared window biological tissue and low fluorescence quantum yield that leads to good generation efficiency. After Bodipy-initiated ring-opening polymerization lactide, polylactide-Bodipy was formulated into PEGylated (NPs) by mixing with PLA-PEG at different concentrations. Formulated NPs around 100 nm...
Envenoming due to Loxosceles spider bites still remains a neglected disease of particular medical concern in the Americas. To date, there is no consensus for treatment envenomed patients, yet horse polyclonal antivenoms are usually infused patients with identified severe conditions. It widely known that venom proteins 30–35 kDa range sphingomyelinase D (SMasesD) activity, reproduce most toxic effects observed loxoscelism. Hence, we believe monoclonal antibody fragments targeting such toxins...