A5033552144- Nanoparticle-Based Drug Delivery
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Pregnancy and preeclampsia studies
- HIV Research and Treatment
- Protein Interaction Studies and Fluorescence Analysis
- Reproductive System and Pregnancy
- Cholinesterase and Neurodegenerative Diseases
- Supramolecular Self-Assembly in Materials
- Graphene and Nanomaterials Applications
- Surfactants and Colloidal Systems
- Pregnancy and Medication Impact
- HIV/AIDS drug development and treatment
- Barrier Structure and Function Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Dendrimers and Hyperbranched Polymers
- Ocular Diseases and Behçet’s Syndrome
- Blood properties and coagulation
- MicroRNA in disease regulation
- Analytical Chemistry and Chromatography
- Flavonoids in Medical Research
Unité de Technologies Chimiques et Biologiques pour la Santé
2015-2023
Inserm
2015-2023
Université Paris Cité
2005-2023
Centre National de la Recherche Scientifique
2013-2023
Délégation Paris 5
2016-2021
Chimie ParisTech
2017-2018
Université Paris Sciences et Lettres
2017-2018
Sorbonne Paris Cité
2017-2018
Sorbonne Université
2018
Université Paris-Sud
2005-2015
The inhibition of the caspase-3 enzyme is reported to increase neuronal cell survival following cerebral ischemia. peptide Z-DEVD-FMK a specific caspase inhibitor, which significantly reduces vulnerability death. However, this molecule unable cross blood−brain barrier (BBB) and diffuse into brain tissue. Thus, development an effective delivery system needed provide sufficient drug concentration prevent Using avidin (SA)−biotin (BIO) technology, we describe here design chitosan (CS)...
Caspases play an important role as mediators of cell death in acute and chronic neurological disorders. Although peptide inhibitors caspases provide neuroprotection, they have to be administered intracerebroventricularly because cannot cross the blood–brain barrier (BBB). Herein, we present a nanocarrier system that can transfer chitosan nanospheres loaded with N -benzyloxycarbonyl-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-fluoromethyl ketone (Z-DEVD-FMK), relatively specific caspase-3 inhibitor,...
Previous in vivo observations rats have shown that poly(ethylene glycol) polyhexadecylcyanoacrylate (PEG-PHDCA) nanoparticles could translocate into the brain after intravenous injection, which (PHDCA) did not. Through detailed analysis of plasma protein adsorption onto surface PEG-PHDCA nanoparticles, present study aimed at clarifying mechanism by penetrate rat endothelial cells (RBEC). Two-dimensional polyacrylamide gel electrophoresis and Western blotting revealed that, incubation with...
We have demonstrated that the polyethylene glycol (PEG) corona of long-circulating polymeric nanoparticles (NPs) favors interaction with amyloid-beta (Aβ(1-42)) peptide both in solution and serum. The influence PEGylation poly(alkyl cyanoacrylate) poly(lactic acid) NPs on monomeric soluble oligomeric forms Aβ(1-42) was by capillary electrophoresis, surface plasmon resonance, thioflavin T assay, confocal microscopy, where binding affected aggregation kinetics. capture serum also evidenced...
A versatile and efficient functionalization strategy for polymeric nanoparticles (NPs) has been reported successfully applied to PEGylated, biodegradable poly(alkyl cyanoacrylate) (PACA) nanocarriers. The relevance of this platform was demonstrated in both the fields cancer Alzheimer's disease (AD). Prepared by copper-catalyzed azide–alkyne cycloaddition (CuAAC) subsequent self-assembly aqueous solution amphiphilic copolymers, resulting functionalized NPs exhibited requisite characteristics...
Although growth factors and anti-apoptotic peptides have been shown to be neuroprotective in stroke models, translation of these experimental findings clinic is hampered by limited penetration the brain. Here, we show that a large peptide like basic fibroblast factor (bFGF) small inhibitor caspase-3 (z-DEVD-FMK) can effectively transported brain after systemic administration incorporating brain-targeted nanoparticles (NPs). Chitosan NPs were loaded with then functionalized conjugating...
Liposomes constitute the most exploited drug-nanocarrier with several liposomal drugs on market. Microfluidic-based preparation methods stand up as a promising approach high reproducibility and ability to scale up. In this study, liposomes composed of DOPC, cholesterol, DSPE-PEG 2000 different molar ratios were fabricated using microfluidic system. Process conditions optimized by applying design experiments (DoE) principles. Furthermore, data used build an artificial neural network (ANN)...
Abstract The biodistribution of colloidal carriers after their administration in vivo depends on the adsorption some plasma proteins and apolipoproteins surface. Poly(methoxypolyethyleneglycol cyanoacrylate‐ co ‐hexadecylcyanoacrylate) (PEG‐PHDCA) nanoparticles have demonstrated capacity to cross blood–brain barrier (BBB) by a mechanism endocytosis. In order clarify this at molecular level, especially adsorbed surface PEG‐PHDCA were analyzed complementary methods such as CE Protein...
In this study, we tested the efficiency of an intravitreal injection tamoxifen, a non-steroidal estrogen receptor modulator, in retinal soluble antigen (S-Ag)-induced experimental autoimmune uveoretinitis (EAU). To increase bioavailability incorporated tamoxifen into polyethylene glycol (PEG)-coated nanoparticles (NP-PEG-TAM). The localization within eye was investigated using fluorescent-labeled PEG-coated after vitreous cavity rats with EAU. Some were distributed extracellularly throughout...
Poly(MePEG2000cyanoacrylate-co-hexadecylcyanoacrylate) (PEG-PHDCA) nanoparticles have demonstrated their capacity to reach the rat central nervous system after intravenous injection. For insight into transport of colloidal systems across blood-brain barrier (BBB), we developed a relevant in vitro BBB model consisting coculture brain endothelial cells (RBECs) and astrocytes. The RBECs used our displayed retained structural characteristics cells, such as expression P-glycoprotein, occludin...
(1) Background: Glioblastoma (GBM) is the most frequent cerebral tumor. It almost always relapses and there no validated treatment for second-line GBM. We proposed coencapsulation of fisetin cisplatin into liposomes, aiming to (i) obtain a synergistic effect by combining anti-angiogenic with cytotoxic cisplatin, (ii) administrate fisetin, highly insoluble in water. The design liposomal formulation able encapsulate, retain deliver both drugs appeared challenge. (2) Methods: Liposomes...
Rhodamine B-tagged poly(alkyl cyanoacrylate) amphiphilic copolymers have been synthesised, characterised and successfully used to prepare fluorescent nanoparticles for human brain endothelial cell imaging, allowing their uptake intracellular trafficking be finely observed.
A novel application of capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was proposed to efficiently detect and monitor the interaction between polymeric nanoparticles β-Amyloid peptide (Aβ1−42), a biomarker for Alzheimer's Disease (AD), at concentrations close physiological conditions. The CE-LIF method allowed PEGylated poly(alkyl cyanoacrylate) (NPs) soluble Aβ1−42 monomers be highlighted. These results were confirmed by surface plasmon resonance (SPR) confocal...