A5103010747- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- DNA and Nucleic Acid Chemistry
- Dendrimers and Hyperbranched Polymers
- Virus-based gene therapy research
- Advanced Polymer Synthesis and Characterization
- DNA Repair Mechanisms
- RNA Research and Splicing
- Nanoplatforms for cancer theranostics
- Angiogenesis and VEGF in Cancer
- Sarcoma Diagnosis and Treatment
- Protein Interaction Studies and Fluorescence Analysis
- Cancer therapeutics and mechanisms
- Colorectal Cancer Surgical Treatments
- Neurological Disorders and Treatments
- Metal complexes synthesis and properties
- Spectroscopy and Chemometric Analyses
- CRISPR and Genetic Engineering
- HER2/EGFR in Cancer Research
- Colorectal and Anal Carcinomas
- RNA and protein synthesis mechanisms
- Gastric Cancer Management and Outcomes
- Synthesis and Characterization of Heterocyclic Compounds
- Spectroscopy Techniques in Biomedical and Chemical Research
Moscow Clinical Scientific Center
2022-2024
Institut Galien Paris-Saclay
2012-2021
Centre National de la Recherche Scientifique
2000-2016
Université Paris-Saclay
2016
Stabilité génétique et oncogenèse
2016
Université Paris-Sud
2012-2016
Softmat - Chimie des colloïdes, polymères & assemblages complexes
2015
Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses
2013
Institut Gustave Roussy
2000-2009
BioReliance (United States)
2003-2007
We identified that the chemical linkage of anticancer drug doxorubicin onto squalene, a natural lipid precursor cholesterol's biosynthesis, led to formation squalenoyl (SQ-Dox) nanoassemblies 130-nm mean diameter, with an original "loop-train" structure. This unique nanomedicine demonstrates: (i) high payload, (ii) decreased toxicity coupled compound, (iii) improved therapeutic response, (iv) use biocompatible transporter material, and (v) ease preparation, all criteria are not combined in...
A versatile and efficient functionalization strategy for polymeric nanoparticles (NPs) has been reported successfully applied to PEGylated, biodegradable poly(alkyl cyanoacrylate) (PACA) nanocarriers. The relevance of this platform was demonstrated in both the fields cancer Alzheimer's disease (AD). Prepared by copper-catalyzed azide–alkyne cycloaddition (CuAAC) subsequent self-assembly aqueous solution amphiphilic copolymers, resulting functionalized NPs exhibited requisite characteristics...
Abstract Vitamin C (Vit C) benefits to human skin physiology notably by stimulating the biosynthesis of collagen. The main cutaneous collagens are types I and III, which less synthesized with aging. Vit is one promotors collagen formation but it poorly bypasses epidermis stratum corneum barrier. To address this challenge, we developed a lipophilic version for improving diffusion delivery. was covalently conjugated squalene (SQ), natural lipid skin, forming novel C–SQ derivative suitable...
Magic rubber bullets: Novel anticancer nanoparticles made of well-defined polymer–drug conjugate amphiphiles are prepared by the controlled growth a hydrophobic polyisoprene chain from drug macroinitiator using nitroxide-mediated polymerization (NMP; see picture). The resulting conjugates self-assembled into exhibiting high payloads and significant activities both in vitro vivo. As service to our authors readers, this journal provides supporting information supplied authors. Such materials...
Metal–organic frameworks (MOFs) are coordination polymers of interest for biomedical applications. Of particular importance, nanoparticles made iron(III) trimesate (MIL-100, MIL standing Material Institut Lavoisier) (nanoMOFs) can be conveniently synthesised under mild and green conditions. They were shown to biodegradable, biocompatible efficient encapsulate a variety active molecules. We have addressed here the challenges highly hydrophilic anticancer prodrug, phosphated gemcitabin...
Drug delivery of combined cytotoxic and antivascular chemotherapies in multidrug nanoassemblies may represent an attractive way to improve the treatment experimental cancers. Here we made proof concept this approach on LS174-T human colon carcinoma xenograft nude mice model. Briefly, have nanoprecipitated anticancer compound gemcitabine conjugated with squalene (SQ-gem) together isocombretastatin A-4 (isoCA-4), a new isomer combretastatin (CA-4). It was found that these molecules...
Fluorescent and biotinylated squalene-gemcitabine prodrug nanoparticles exhibiting high drug payloads have been prepared successfully used to target different cancer cell lines, resulting in increased uptake improved anticancer efficiency, which represents the first targeted system derived from squalenoylation approach.
The synthesis of a novel class polymer prodrug nanoparticles with anticancer activity is reported by using squalene, naturally occurring isoprenoid, as building block the reversible addition–fragmentation (RAFT) technique. RAFT agent was functionalized gemcitabine (Gem) drug, and polymerization squalenyl-methacrylate (SqMA) led to well-defined macromolecular prodrugs comprising one Gem at extremity each chain. amphiphilic nature resulting Gem–PSqMA conjugates allowed them self-assemble into...
Abstract Background The application of synthetic vectors for gene transfer has potential advantages over virus‐based systems. Their use, however, is limited since they generally lack the efficiency achieved with recombinant viral such as adenovirus. Polyamidoamine (PAMAM) and phosphorus‐containing dendrimers (P‐dendrimers) are specific polymers a defined spherical structure. They bind to DNA through electrostatic interactions thus forming complexes that efficiently transfect cells in vitro ....
Abstract Gene transfer is an attractive approach to fight cancer by targeting cells or their vasculature. Our study reports the inhibition of tumor growth and angiogenesis a nonviral method using dendrimers associated with 36‐mer anionic oligomers (ON36) for delivering angiostatin (Kringle 1–3) tissue inhibitor metalloproteinase (TIMP)‐2 genes. The optimal concentrations ON36 efficient green fluorescent protein (GFP) plasmid delivery in endothelial (HMEC‐1) (MDA‐MB‐435) were first chosen....
The multifunctional DNA repair enzymes apurinic/apyrimidinic (AP) endonucleases cleave at AP sites and 3′-blocking moieties generated by glycosylases in the base excision pathway. Alternatively, nucleotide incision (NIR) pathway, same incise 5′ of a number oxidatively damaged bases. At present, physiological relevance latter function remains unclear. Here, we report genetic dissection endonuclease functions NIR pathways. Three mutants Escherichia coli IV (Nfo), carrying amino acid...
A series of new lipid prodrugs paclitaxel, which can be formulated as nanoassemblies, are described. These designed to overcome the limitations due systemic toxicity and low water solubility paclitaxel consist a squalene chain bound 2'-OH through 1,4-cis,cis-dienic linker. This design allows squalene-conjugates self-assemble nanoparticular systems while preserving an efficient release free drug, thanks dienic spacer. The size, steric hindrance, functional groups spacer have been modulated....
A new paclitaxel (Ptx) prodrug was designed by coupling a single terpene unit (MIP) to the hydroxyl group in position 2' of drug molecule. Using squalene derivative polyethylene glycol (SQ-PEG) as surface active agent, resulting bioconjugate (PtxMIP) self-assembled water leading formation stable nanoparticles (PtxMIP_SQ-PEG NPs) with an impressively high loading (82%). In vivo, anticancer activity this novel Ptx nanoassembled compared conventional Cremophor-containing formulation (Taxol) on...
Oxidatively damaged DNA bases are substrates for two overlapping repair pathways: glycosylase-initiated base excision (BER) and apurinic/apyrimidinic (AP) endonuclease-initiated nucleotide incision (NIR). In the BER pathway, an AP endonuclease cleaves at sites 3'-blocking moieties generated by glycosylases, whereas in NIR same incises 5' to oxidized base. The majority of characterized endonucleases possess classic activities, approximately a half them can also have activity. At present,...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSignificant Tumor Growth Inhibition from Naturally Occurring Lipid-Containing Polymer Prodrug Nanoparticles Obtained by the Drug-Initiated MethodAndrei Maksimenko, Duc Trung Bui, Didier Desmaële, Patrick Couvreur, and Julien Nicolas*View Author Information Institut Galien Paris-Sud, Université UMR CNRS 8612, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, F-92296 Châtenay-Malabry cedex, France*E-mail: [email protected]Cite this: Chem....
RET/PTC1 is the most prevalent type of gene rearrangement found in papillary thyroid carcinoma (PTC). Previously, we introduced a new noncationic nanosystem for targeted silencing by efficient delivery small interfering RNA (siRNA) using "squalenoylation" approach. With aim improving these results further, designed squalenoyl nanostructures consisting fusogenic peptide GALA-cholesterol (GALA-Chol) and squalene (SQ) nanoparticles (NPs) siRNA RET/PTC1.The RET/PTC1-SQ bioconjugate was...
We have used structured antisense oligonucleotides (AON), which are protected against extra and intracellular degradation by their internal structure. shown that if correctly designed this structure does not prevent them from hybridizing to the mRNA target. This concept allows reducing number of thioate groups in oligonucleotide therefore potential toxicity. Junction oncogenes found cancers such as certain leukemias, Ewing sarcoma, thyroid papillary carcinomas. sarcoma is a cancer children...
Lipid peroxidation generates aldehydes, which react with DNA bases, forming genotoxic exocyclic etheno(epsilon)-adducts. E-bases have been implicated in vinyl chloride-induced carcinogenesis, and increased levels of these lesions formed by endogenous processes are found human degenerative disorders. E-adducts repaired the base excision repair pathway. Here, we report efficient biological hijacking alkyl-N-purine-DNA glycosylase (ANPG) 3,N(4)-ethenocytosine (epsilonC) when present DNA. Unlike...