A5078365658- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Complement system in diseases
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Advanced Drug Delivery Systems
- Monoclonal and Polyclonal Antibodies Research
- Nanoplatforms for cancer theranostics
- Inhalation and Respiratory Drug Delivery
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Immune Cell Function and Interaction
- Surfactants and Colloidal Systems
- Platelet Disorders and Treatments
- SARS-CoV-2 and COVID-19 Research
- Dendrimers and Hyperbranched Polymers
- Cell Adhesion Molecules Research
- Supramolecular Self-Assembly in Materials
- 3D Printing in Biomedical Research
Newcastle University
2017-2025
University of Colorado Anschutz Medical Campus
2018-2025
University of Montana
2018-2025
Clinical Research Institute
2025
University of Newcastle Australia
2025
University of Copenhagen
2011-2021
Oslo University Hospital
2019
De Montfort University
2019
University of Colorado Denver
2017-2018
Andover Eye Associates
2018
Poly(ethylenimine) (PEI) is a cationic macromolecule commonly used in gene transfer/therapy protocols with high transfection efficiency both vitro and vivo. PEI also cytotoxic, but the molecular basis of its cytotoxicity poorly understood. Here, we have demonstrated that branched (25 kDa) linear (750 can induce membrane damage initiate apoptosis three clinically relevant human cell lines (Jurkat T cells, umbilical vein endothelial THLE3 hepatocyte-like cells). We defined Phase I toxicity as...
Polycations such as polyethylenimine (PEI) are used in many novel nonviral vector designs and there continuous efforts to increase our mechanistic understanding of their interactions with cells. Even so, the mechanism polyplex escape from endosomal/lysosomal pathway after internalization is still elusive. The "proton sponge " hypothesis remains most generally accepted mechanism, although it heavily debated. This associated large buffering capacity PEI other polycations, which has been...
Remarkable progress has recently been made in the synthesis and characterization of engineered nanoparticles for imaging treatment cancers, resulting several promising candidates clinical trials. Despite these advances, applications nanoparticle-based therapeutic/imaging agents remain limited by biological, immunological, translational barriers. In order to overcome existing status quo drug delivery, there is a need open frank discussion nanomedicine community on what needed make qualitative...
Nanoparticles with surface projected polyethyleneoxide (PEO) chains in "mushroom−brush" and "brush" configurations display stealth properties systemic circulation have numerous applications site-specific targeting for controlled drug delivery release as well diagnostic imaging. We report on the "structure−activity" relationship pertaining to surface-immobilized PEO of various model nanoparticles, initiation complement cascade, which is most ancient component innate human immunity, its...
In recent years, steady progress has been made in synthesizing and characterizing engineered nanoparticles, resulting several approved drugs multiple promising candidates clinical trials. Regulatory agencies such as the Food Drug Administration European Medicines Agency released important guidance documents facilitating nanoparticle-based drug product development, particularly context of liposomes lipid-based carriers. Even with achieved, it is clear that many barriers must still be overcome...