A5003352063- Complement system in diseases
- Drug-Induced Adverse Reactions
- Food Allergy and Anaphylaxis Research
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 and COVID-19 Research
- Inhalation and Respiratory Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Allergic Rhinitis and Sensitization
- Lipid Membrane Structure and Behavior
- Hemoglobin structure and function
- Immunotherapy and Immune Responses
- HIV/AIDS drug development and treatment
- Biosimilars and Bioanalytical Methods
- HIV Research and Treatment
- Advanced Drug Delivery Systems
- Mast cells and histamine
- Neonatal Health and Biochemistry
- Platelet Disorders and Treatments
- Asthma and respiratory diseases
- Erythrocyte Function and Pathophysiology
- Protein Interaction Studies and Fluorescence Analysis
- Nanoparticles: synthesis and applications
- Contact Dermatitis and Allergies
Semmelweis University
2016-2025
Sungkyunkwan University
2023-2025
University of Miskolc
2015-2024
Institute of Molecular Life Sciences
2021
University of Pecs
2018
Bay Zoltán Foundation for Applied Research
2008-2012
Hungarian Academy of Sciences
2006-2011
Henry M. Jackson Foundation
2007
Walter Reed Army Institute of Research
1997-2006
National Cancer Institute
1989-2005
Polyethylene glycol (PEG)-coated nanopharmaceuticals can cause mild to severe hypersensitivity reactions (HSRs), which occasionally be life threatening or even lethal. The phenomenon represents an unsolved immune barrier the use of these drugs, yet its mechanism is poorly understood. This study showed that a single i.v. injection in pigs low dose PEGylated liposomes (Doxebo) induced massive rise anti-PEG IgM blood, peaking at days 7–9 and declining over 6 weeks. Bolus injections...
PEGylation of lipid-based nanoparticles and other nanocarriers is widely used to increase their stability plasma half-life. However, either pre-existing or de novo formed anti-PEG antibodies can induce hypersensitivity reactions accelerated blood clearance through binding the nanoparticle surfaces, leading activation complement system. In this study, we investigated consequences mechanisms by interacting with different types PEGylated nanoparticles. By using both liposomes loaded (model)...
Despite the worldwide success of mRNA-LNP Covid-19 vaccines, nanoscale structures these formulations are still poorly understood. To fill this gap, we used a combination atomic force microscopy (AFM), dynamic light scattering (DLS), transmission electron (TEM), cryogenic (cryo-TEM), and determination intra-LNP pH gradient to analyze nanoparticles (NPs) in BNT162b2 (Comirnaty), comparing it with well-characterized PEGylated liposomal doxorubicin (Doxil). Comirnaty NPs had similar size...
Background: Cancer patients treated with the anticancer drug, paclitaxel (Taxol) often experience mild to severe hypersensitivity reactions. It is not known how these reactions are induced and whether inducer or its vehicle (i.e., Cremophor EL in 50% ethanol). Molecules present similar structure certain nonionic block copolymers that activate complement proteins involved various immune processes). To explore role of observed reactions, we studied effects plus ethanol on human vitro. Methods:...
Pegylated liposomal doxorubicin (Doxil) and 99mTc-HYNIC PEG liposomes (HPL) were reported earlier to cause hypersensitivity reactions (HSRs) in a substantial percentage of patients treated i.v. with these formulations. Here we report that (1) Doxil, HPL, pegylated phosphatidylethanolamine (PEG-PE)-containing empty matched Doxil HPL size lipid composition, phosphatidylglycerol (PG)-containing negatively charged vesicles potent C activators human serum vitro, whereas small neutral caused no...
Methoxy(polyethylene glycol), mPEG, -grafted liposomes are known to exhibit prolonged circulation time in the blood, but their infusion into a substantial percentage of human subjects triggers immediate non-IgE-mediated hypersensitivity reactions. These reactions strongly believed arise from anaphylatoxin production through complement activation. Despite general view that vesicle surface camouflaging with mPEG should dramatically suppress activation, here we show bilayer enrichment...