A5048645957- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Endodontics and Root Canal Treatments
- Oral microbiology and periodontitis research
- Vector-borne infectious diseases
- Toxin Mechanisms and Immunotoxins
- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- Dental Radiography and Imaging
- Vector-Borne Animal Diseases
- Folate and B Vitamins Research
- Congenital heart defects research
- Dental materials and restorations
- Peptidase Inhibition and Analysis
- Lung Cancer Research Studies
- Cancer, Hypoxia, and Metabolism
Hebei University of Technology
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2024
Peking Union Medical College Hospital
2017-2023
Tianjin Normal University
1996-2022
RELX Group (United States)
2020
Fudan University
2020
State Key Laboratory of Molecular Oncology
2018
Shanghai Jiao Tong University
2009-2017
Shanghai Ninth People's Hospital
2009-2017
American Association of Endodontists
2016
We have developed a high-throughput method for analyzing the methylation status of hundreds preselected genes simultaneously and applied it to discovery signatures that distinguish normal from cancer tissue samples. Through an adaptation GoldenGate genotyping assay implemented on BeadArray platform, state 1536 specific CpG sites in 371 (one nine per gene) was measured single reaction by multiplexed 200 ng bisulfite-treated genomic DNA. The used obtain quantitative measure level at each site....
Midzone microtubules of mammalian cells play an essential role in the induction cell cleavage, serving as a platform for number proteins that part cytokinesis. We demonstrate PRC1, mitotic spindle-associated Cdk substrate is to microtubule binding and bundling protein both vivo vitro. Overexpression PRC1 extensively bundles interphase microtubules, but does not affect early spindle organization. contains two phosphorylation motifs, possibly important suppression bundling,...
Microtubule (MT)-based motor proteins, kinesins and dyneins, play important roles in multiple cellular processes including cell division. In this study, we describe the generation use of an Escherichia coli RNase III-prepared human kinesin/dynein esiRNA library to systematically analyze functions all MT proteins. Our results indicate that at least 12 are involved mitosis cytokinesis. Eg5 (a member kinesin-5 family), Kif2A kinesin-13 KifC1 kinesin-14 family) crucial for spindle formation;...
Mutations in PARKIN, pten-induced putative kinase 1 (PINK1), and DJ-1 are individually linked to autosomal recessive early-onset familial forms of Parkinson disease (PD). Although mutations these genes lead the same state, functional relationships between them how their respective disease-associated cause PD largely unknown. Here, we show that Parkin, PINK1, formed a complex (termed PPD complex) promote ubiquitination degradation Parkin substrates, including itself Synphilin-1 neuroblastoma...
We have examined DNA from four human esophageal carcinoma cell lines and 50 primary carcinomas obtained China, Italy, France for amplification of the cyclin D1 gene. also 36 these expression retinoblastoma (RB) proteins by immunohistochemistry. found a 3- to 10-fold gene in 16 (32%) tumors two lines. Cyclin protein was overexpressed 12 13 that showed when compared normal controls. Studies on RB indicated 6 (17%) tumor samples one line did not show detectable this protein. The had...
The spindle midzone, a conspicuous network of antiparallel interdigitating nonkinetochore microtubules between separating chromosomes, plays crucial role in regulating the initiation and completion cytokinesis. In this study, we report use time-lapse microscopy human kinesin endoribonucleases RNase III-prepared short interfering RNA (esiRNA) library to identify Kif4 as motor protein that translocates PRC1, midzone-associated cyclin-dependent kinase substrate protein, plus ends during...
We have characterized HsCdc6, a human protein homologous to the budding yeast Cdc6p that is essential for DNA replication. show that, unlike Cdc6p, levels of HsCdc6 remain constant throughout cell cycle in cells. However, phosphorylation regulated during cycle. an excellent substrate Cdk2 vitro and phosphorylated vivo at three sites (Ser-54, Ser-74, Ser-106) are by , strongly suggesting Cdk substrate. nuclear G 1 but translocates cytoplasm start S phase via Crm1-dependent export. An...
The inability of heart muscle to regenerate by replication existing cardiomyocytes has engendered considerable interest in identifying developmental or other stimuli capable sustaining the proliferative capacity immature stimulating division postmitotic cardiomyocytes. Here, we demonstrate that reactivation Notch signaling causes embryonic stem cell–derived and neonatal ventricular enter cell cycle. response declines as they mature, such late activation triggers DNA damage checkpoint G2/M...
Side population (SP) cells may play an important role in tumorigenesis and cancer therapy. We isolate identify the stem-like human esophageal carcinoma (EC) cell lines, EC9706 EC109 labeled with Hoechst 33342. Both lines contained SP cells, that had strongest dye efflux activity ("Tip"-SP cell) higher clone formation efficiency than non-SP cells. When transplanted into nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, "Tip"-SP showed at least 50 times tumorigenicity...
Microtubule (MT) kinesin motor proteins orchestrate various cellular processes (e.g. mitosis, motility and organelle transportation) have been implicated in human carcinogenesis. Kif18A, a plus-end directed MT depolymerase kinesin, regulates dynamics, chromosome congression cell division. In this study, we report that Kif18A is overexpressed breast cancers overexpression associated with tumor grade, metastasis poor survival. Functional analyses reveal ectopic of results multinucleation,...
The cyclin-dependent kinase (CDK) inhibitor p21 is induced by the tumor suppressor gene product p53 and thought to be important for arrest of cell cycle following DNA damage. Here we have investigated contribution in inhibiting different cyclin-CDK complexes that drive transitions UV irradiation-induced damage normal human fibroblasts immortalized rodent fibroblasts. When cells were exposed a low dose irradiation, both induced; protein activities associated with Cdc2, Cdk2, Cdk4 inhibited;...
ABSTRACT Activation of Cdc2, is the universal event controlling onset mitosis. In higher eukaryotes, Cdc2 activity in part regulated by inhibitory phosphorylation Thr14 and Tyr15, catalyzed Wee1 Myt1, which prevents catastrophic premature entry into this study we defined function Myt1 overexpression studies both S. pombe a human osteosarcoma cell line. Similar to Wee1, prevented mitosis types; however, catalytic was not essential for cycle delay observed with cells. expression restricted...
We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate protein, in regulating spatiotemporal formation midzone HeLa cells. Cdk-mediated phosphorylation PRC1 early mitosis holds an inactive monomeric state. During metaphase-to-anaphase transition, is dephosphorylated, promoting oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments, we demonstrate that dephosphorylated...
We report the identification of Cdc7/Dbf4 phosphorylation sites in human MCM2 and determination role initiation DNA replication. Using immunoblotting, immunofluorescence, high-speed automated cell-imaging analyses with antibodies specific against phosphorylated MCM2, we show that chromatin recruitment are regulated during cell cycle HeLa cells. Chromatin-bound is by G1/S, which coincides Moreover, baculovirus-expressed purified MCM2-7 complex its phosphomimetic MCM2E-7 display higher ATPase...