Shuaiyao Lu
A5077484550- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Immunology Research
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Pluripotent Stem Cells Research
- Viral Infections and Vectors
- interferon and immune responses
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Pancreatic function and diabetes
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2025
Yunnan Center for Disease Control And Prevention
2025
Institute of Medical Biology
2014-2025
Kunming Institute of Zoology
2020-2024
Pennsylvania State University
2023-2024
Peking Union Medical College Hospital
2014-2024
Huazhong Agricultural University
2023
Academy of Medical Sciences
2023
Chinese Institute for Brain Research
2023
Sichuan University
2022
As the emerging variants of SARS-CoV-2 continue to drive worldwide pandemic, there is a constant demand for vaccines that offer more effective and broad-spectrum protection. Here, we report circular RNA (circRNA) vaccine elicited potent neutralizing antibodies T cell responses by expressing trimeric RBD spike protein, providing robust protection against in both mice rhesus macaques. Notably, circRNA enabled higher durable antigen production than 1mΨ-modified mRNA proportion distinct...
We have examined DNA from four human esophageal carcinoma cell lines and 50 primary carcinomas obtained China, Italy, France for amplification of the cyclin D1 gene. also 36 these expression retinoblastoma (RB) proteins by immunohistochemistry. found a 3- to 10-fold gene in 16 (32%) tumors two lines. Cyclin protein was overexpressed 12 13 that showed when compared normal controls. Studies on RB indicated 6 (17%) tumor samples one line did not show detectable this protein. The had...
Identification of a suitable nonhuman primate (NHP) model COVID-19 remains challenging. Here, we characterized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in three NHP species: Old World monkeys Macaca mulatta (M. mulatta) and fascicularis fascicularis) New monkey Callithrix jacchus (C. jacchus). Infected M. showed abnormal chest radiographs, an increased body temperature decreased weight. Viral genomes were detected swab blood samples from all animals. load was...
Abstract Azvudine (FNC) is a nucleoside analog that inhibits HIV-1 RNA-dependent RNA polymerase (RdRp). Recently, we discovered FNC an agent against SARS-CoV-2, and have taken it into Phase III trial for COVID-19 patients. monophosphate inhibited SARS-CoV-2 HCoV-OC43 coronavirus with EC 50 between 1.2 4.3 μM, depending on viruses or cells, selective index (SI) in 15–83 range. Oral administration of rats revealed substantial thymus-homing feature, triphosphate (the active form) concentrated...
Breakthrough infections by SARS-CoV-2 variants become the global challenge for pandemic control. Previously, we developed protein subunit vaccine ZF2001 based on dimeric receptor-binding domain (RBD) of prototype SARS-CoV-2. Here, a chimeric RBD-dimer approach to adapt variants. A prototype-Beta was first designed resistant Beta variant. Compared with its homotypic forms, elicited broader sera neutralization and conferred better protection in mice. The further verified macaques. This...
Abstract Neurological manifestations are frequently reported in the COVID-19 patients. Neuromechanism of SARS-CoV-2 remains to be elucidated. In this study, we explored mechanisms neurotropism via our established non-human primate model COVID-19. rhesus monkey, invades CNS primarily olfactory bulb. Thereafter, viruses rapidly spread functional areas central nervous system, such as hippocampus, thalamus, and medulla oblongata. The infection induces inflammation possibly by targeting neurons,...
Although inoculation of COVID-19 vaccines has rolled out globally, there is still a critical need for safe and effective to ensure fair equitable supply all countries. Here, we report on the development highly efficacious mRNA vaccine, SW0123 that composed sequence-modified encoding full-length SARS-CoV-2 Spike protein packaged in core-shell structured lipopolyplex (LPP) nanoparticles. easy produce using large-scale microfluidics-based apparatus. The unique nanoparticle facilitates vaccine...
Abstract COVID-19, caused by SARS-CoV-2 infection, has recently been announced as a pandemic all over the world. Plenty of diagnostic, preventive and therapeutic knowledges have enriched from clinical studies since December 2019. However, animal models, particularly non-human primate are urgently needed for critical questions that could not be answered in patients, evaluations anti-viral drugs vaccines. In this study, two families primates, Old world monkeys (12 Macaca mulatta , 6...
Abstract Since severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) became a pandemic event in the world, it has not only caused huge economic losses, but also serious threat to global public health. Many scientific questions about SARS-CoV-2 and Coronavirus disease (COVID-19) were raised urgently need be answered, including susceptibility of animals infection. Here we tested whether tree shrew, an emerging experimental animal domesticated from wild animal, is susceptible No clinical...
Abstract The recently emerged Omicron (B.1.1.529) variant has rapidly surpassed Delta to become the predominant circulating SARS-CoV-2 variant, given higher transmissibility rate and immune escape ability, resulting in breakthrough infections vaccinated individuals. A new generation of vaccines targeting are urgently needed. Here, we developed a subunit vaccine named RBD-HR/trimer by directly linking sequence RBD derived from (containing L452R T478K) HR1 HR2 S2 tandem manner, which can...
Abstract The COVID-19 response strategies in Chinese mainland were recently adjusted due to the reduced pathogenicity and enhanced infectivity of Omicron subvariants. In Chengdu, China, an infection wave was predominantly induced by BA.5 subvariant. It is crucial determine whether hybrid anti-SARS-CoV-2 immunity following infection, coupled with a variety immune background, sufficient shape responses against newly emerged subvariants, especially for XBB lineages. To investigate this, we...
The emergence of a series SARS-CoV-2 variants has necessitated the search for broad-spectrum antiviral targets. aryl hydrocarbon receptor (AhR) senses tryptophan metabolites and is an immune regulator. However, role AhR in infection whether can be used as target therapy against its are yet unclear. Here, we show that with activates signaling facilitates viral replication by interfering IFN-I-driven immunity up-regulating ACE2 expression. pharmacological blockade or knockout reduces variants'...
Abstract Herd immunity achieved through mass vaccination is an effective approach to prevent contagious diseases. Nonetheless, emerging SARS-CoV-2 variants with frequent mutations largely evaded humoral induced by Spike-based COVID-19 vaccines. Herein, we develop a lipid nanoparticle (LNP)-formulated mRNA-based T-cell-inducing antigen, which targeted three proteome regions that enriched human HLA-I epitopes (HLA-EPs). Immunization of HLA-EPs induces potent cellular responses infection in...
Abstract Mucosal immunity plays a significant role in the first-line defense against viruses transmitted and infected through respiratory system, such as SARS-CoV-2. However, lack of effective safe adjuvants currently limits development COVID-19 mucosal vaccines. In current study, we prepare an intranasal vaccine containing cationic crosslinked carbon dots (CCD) SARS-CoV-2 antigen, RBD-HR with spontaneous antigen particlization. Intranasal immunization CCD/RBD-HR induces high levels...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of disease-19 patients, although some do not express angiotensin-converting enzyme-2 (ACE2), a known receptor SARS-CoV-2, implying the presence alternative receptors and/or co-receptors. Here, we show that ubiquitously distributed human transferrin (TfR), which binds to diferric traffic between membrane and endosome for iron delivery cycle, can ACE2-independently mediate SARS-CoV-2...
Abstract The emergence of XBB- and JN.1-lineages with remarkable immune evasion characteristics have led to rises in breakthrough infections within populations. In addition, the unfavorable impacts imprinting, stemming from continuous exposure antigens circulated viruses, been observed incline response against earlier lineages, thereby declining neutralization newly emerged Omicron subvariants. this, advancement next-generation vaccines COVID-19 targeting components new subvariants such as...
During sporulation of the Gram-positive bacterium Bacillus subtilis, transcription genes encoding spore coat proteins in mother-cell compartment sporangium is controlled by RNA polymerase containing sigma subunit called K. Based on comparison N-terminal amino acid sequence K with nucleotide gene (sigK), primary product sigK was inferred to be a pro-protein (pro-sigma K) 20 extra acids at N terminus. Using antibodies generated against pro-sigma K, we have detected beginning third hour and...