A5019841280- Protein purification and stability
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Microfluidic and Capillary Electrophoresis Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Hydrogels: synthesis, properties, applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Phase Equilibria and Thermodynamics
- Advanced Drug Delivery Systems
- Probiotics and Fermented Foods
- Chemical and Physical Properties in Aqueous Solutions
- Polymer Surface Interaction Studies
- Polymer Foaming and Composites
- GABA and Rice Research
- Chromatography in Natural Products
- Glycosylation and Glycoproteins Research
- Microencapsulation and Drying Processes
- Enzyme Structure and Function
- Protein Interaction Studies and Fluorescence Analysis
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
Zhejiang University
2016-2025
Collaborative Innovation Center of Chemical Science and Engineering Tianjin
2014-2015
State Key Laboratory of Chemical Engineering
2008-2012
Zhejiang Gongshang University
2009-2010
Hangzhou Vocational and Technical College
2010
Zhejiang University of Science and Technology
2006-2009
Hua Medicine (China)
2008
Zhejiang Hisun Pharmaceutical (China)
2008
Longyan University
2007
AO Foundation
2007
This study investigated the effect of mechanical load on human mesenchymal stem cell (hMSC) differentiation under different exogenous transforming growth factor-beta1 (TGF-beta(1)) concentrations (0, 1 or 10 ng/ml).The role TGF-beta signalling pathway in this process was also studied. Human MSCs were seeded into fibrin-biodegradable polyurethane scaffolds at a density 5 x 10(6) cells per scaffold and stimulated using our bioreactor. One hour surface motion superimposed cyclic compression...
In this study, human bone marrow mesenchymal stem cell (hMSC)-seeded fibrin-polyurethane composite scaffolds were subjected to various mechanical load protocols determine the effect of compression and surface rotation frequency axial magnitude on induction chondrocyte-specific gene expression protein synthesis. After 7 days preculture load, application dynamic shear 1 h daily enhanced chondrogenesis hMSCs compared with nonloaded control samples. Higher higher amplitude induced...
The glutamate decarboxylase (GAD) system of Lactobacillus brevis involves two isoforms GAD, GadA and GadB, which catalyze the conversion L-glutamate to γ-aminobutyric acid (GABA) in a proton-consuming reaction contributing intracellular pH homeostasis. However, direct experimental evidence for detailed contributions gad genes tolerance GABA production is lacking.Molecular analysis revealed that gadB cotranscribed tandem with upstream gadC, expression gadCB greatly upregulated response low...
This study investigated whether a three-dimensional (3D) fibrin gel–polyurethane scaffold composite can provide an environment for chondrogenesis of human bone marrow mesenchymal stem cells (hMSCs) that is as supportive pellet culture, which established model evaluating chondrogenesis. Pellet culture was carried out in serum-free medium the absence or presence transforming growth factor beta 1 (TGF-β1) and dexamethasone. hMSCs were seeded into gel–biodegradable polyurethane at cell densities...
Abstract Developing an accurate and reliable model for chromatographic separation that meets regulatory requirements ensures consistency in development remains challenging. In order to address this challenge, a standardized approach was proposed study with ion‐exchange chromatography (IEC). The includes the following steps: liquid flow identification, system column‐specific parameters determination validation, multi‐component protein amount steric mass action evaluation, validation of...
A novel microcapsule system composed of sodium cellulose sulfate (NaCS) and chitosan was prepared by layer-by-layer (LbL) self-assembly technique. As a potential drug delivery system, it had several advantages in biocompatibility biodegradation due to its use natural polysaccharides. Some parameters the preparation microcapsule, such as layers LbL process (up 20 layers), viscosities (50, 100, 200 mPas), outmost layer materials (NaCS or chitosan), NaCl concentrations (0 M, 0.5 1 M), were...
Hydrophobic charge-induction chromatography (HCIC) with 4-mercaptoethyl-pyridine (MEP) as the ligand is a novel technology for antibody purification. In present work, molecular simulation methods were used to investigate interactions between MEP and Fc fragment of IgG (Fc-A). Six ligands different structures spacer arm studied docking dynamics at neutral acidic pH. The binding modes interaction energies analyzed. results indicated that all tested could bind into selected pocket on CH2 domain...