A5072976618- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- CRISPR and Genetic Engineering
- Advanced Chemical Sensor Technologies
- Plant Virus Research Studies
- SARS-CoV-2 detection and testing
- Photoacoustic and Ultrasonic Imaging
- Gas Sensing Nanomaterials and Sensors
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Molecular Communication and Nanonetworks
- Plasmonic and Surface Plasmon Research
- Digital Holography and Microscopy
- Advanced Fluorescence Microscopy Techniques
- Advanced Sensor and Energy Harvesting Materials
- Mobile Health and mHealth Applications
- Nanoparticles: synthesis and applications
- Analytical Chemistry and Sensors
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Image Processing Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Cell Image Analysis Techniques
North Carolina State University
2017-2025
North Central State College
2021-2023
Bangladesh University of Engineering and Technology
2022
University of California, Los Angeles
2013-2022
California NanoSystems Institute
2013-2019
Samueli Institute
2017
Xi'an Jiaotong University
2017
UCLA Jonsson Comprehensive Cancer Center
2014
Los Angeles City College
2014
Purdue University West Lafayette
2008-2013
Optical imaging of nanoscale objects, whether it is based on scattering or fluorescence, a challenging task due to reduced detection signal-to-noise ratio and contrast at subwavelength dimensions. Here, we report field-portable fluorescence microscopy platform installed smart phone for individual nanoparticles as well viruses using lightweight compact opto-mechanical attachment the existing camera module cell phone. This hand-held fluorescent device utilizes (i) 450 nm laser diode that...
Detection of environmental contamination such as trace-level toxic heavy metal ions mostly relies on bulky and costly analytical instruments. However, a considerable global need exists for portable, rapid, specific, sensitive, cost-effective detection techniques that can be used in resource-limited field settings. Here we introduce smart-phone-based hand-held platform allows the quantification mercury(II) water samples with parts per billion (ppb) level sensitivity. For this task, created an...
Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely utilized for various nanomedicine, molecular sensing, and disease screening applications, this multiwell plate batched analysis dramatically reduces diagnosis costs per patient compared to nonbatched or nonstandard tests. However, their use in resource-limited field-settings is inhibited by the necessity relatively large expensive readout instruments. To mitigate problem, we created a hand-held cost-effective...
Point-of-care COVID-19 assays that are more sensitive than the current RT-PCR (reverse transcription polymerase chain reaction) gold standard assay needed to improve disease control efforts. We describe development of a portable, ultrasensitive saliva-based with 15-min sample-to-answer time does not require RNA isolation or laboratory equipment. This uses CRISPR-Cas12a activity enhance viral amplicon signal, which is stimulated by laser diode smartphone-based fluorescence microscope device....
Mass production of consumer electronics devices and market competition have provided users with cost-effective, compact, high-performance products high-end components including digital cameras, graphics processing units, various sensors. This opened up the opportunity for researchers, even citizen scientists, to develop novel imaging, sensing, diagnostics platforms using mobile phones as an underlying platform. Mobile phones' hardware software technical capabilities, wireless connectivity,...
In-field molecular diagnosis of plant diseases via nucleic acid amplification is currently limited by cumbersome protocols for extracting and isolating pathogenic DNA from tissues. To address this challenge, a rapid extraction method was developed using disposable polymeric microneedle (MN) patch. By applying MN patches on leaves, amplification-assay-ready can be extracted within minute different species. MN-extracted used direct polymerase chain reaction plastid without purification....
Abstract Emerging plant diseases, caused by pathogens, pests, and climate change, are critical threats to not only the natural ecosystem but also human life. To mitigate crop loss due various biotic abiotic stresses, new sensor technologies monitor health, predict, track diseases in real time desired. Wearable electronics have recently been developed for health monitoring. However, application of wearable agriculture science is its infancy. mean that sensors will be directly placed on...
Wearable plant sensors hold tremendous potential for smart agriculture. We report a lower leaf surface-attached multimodal wearable sensor continuous monitoring of physiology by tracking both biochemical and biophysical signals the its microenvironment. Sensors detecting volatile organic compounds (VOCs), temperature, humidity are integrated into single platform. The abaxial attachment position is selected on basis stomata density to improve signal strength. This versatile platform enables...
DNA imaging techniques using optical microscopy have found numerous applications in biology, chemistry and physics are based on relatively expensive, bulky complicated set-ups that limit their use to advanced laboratory settings. Here we demonstrate length quantification of single molecule strands a compact, lightweight cost-effective fluorescence microscope installed mobile phone. In addition an optomechanical attachment creates high contrast dark-field setup external lens, thin-film...
Abstract Nanosized near infrared (NIR) responsive nanohybrids are synthesized by surface‐initiated atom transfer radical polymerization (SI‐ATRP) of N ‐isopropylacrylamide (NIPAAm) on gold nanorods (Au NRs). Transmission electron microscopy images demonstrate that the have a clear core/shell structure. Temperature‐variable 1 H NMR spectroscopy, quasi‐elastic light scattering (QELS), UV‐vis and laser irradiation prove both temperature NIR responsive. Norvancomycin (NVan) loading release...
Abstract Molecular diagnostics is typically outsourced to well-equipped centralized laboratories, often far from the patient. We developed molecular assays and portable optical imaging designs that permit on-site with a cost-effective mobile-phone-based multimodal microscope. demonstrate targeted next-generation DNA sequencing reactions in situ point mutation detection preserved tumour samples can be imaged analysed using mobile phone microscopy, achieving new milestone for tele-medicine...
Key challenges with point-of-care (POC) nucleic acid tests include achieving a low-cost, portable form factor, and stable readout, while also retaining the same robust standards of benchtop lab-based tests. We addressed two crucial aspects this problem, identifying chemical additive, hydroxynaphthol blue, that both stabilizes significantly enhances intercalator-based fluorescence readout concentration, developing cost-effective fiber-optic bundle-based microplate reader integrated onto...
Developing easy-to-use and miniaturized detectors is essential for in-field monitoring of environmentally hazardous substances, such as the cyanotoxins. We demonstrated a differential fluorescent sensor array made aptamers single-stranded DNA (ssDNA) dyes multiplexed detection discrimination four common cyanotoxins with an ordinary smartphone within 5 min reaction. The assay reagents were preloaded dried in microfluidic chip long shelf life over 60 days. Upon addition analyte solutions,...
The advent of highly sensitive photodetectors and the development photostabilization strategies made detecting fluorescence single molecules a routine task in many labs around world. However, to this day, process requires cost-intensive optical instruments due truly nanoscopic signal emitter. Simplifying single-molecule detection would enable exciting applications, e.g., point-of-care diagnostic settings, where costly equipment be prohibitive. Here, we introduce addressable NanoAntennas with...