A5090813952- Plant Pathogens and Resistance
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Biosensors and Analytical Detection
- Plant Surface Properties and Treatments
- Plant Virus Research Studies
- Advanced Biosensing Techniques and Applications
- Advanced Chemical Sensor Technologies
- Plant Diversity and Evolution
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Species Descriptions
- Agricultural Practices and Plant Genetics
- Fungal Plant Pathogen Control
- Plant and Fungal Interactions Research
- Allergic Rhinitis and Sensitization
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Microbial Inactivation Methods
- Gas Sensing Nanomaterials and Sensors
- Legume Nitrogen Fixing Symbiosis
- Botanical Research and Applications
- Lichen and fungal ecology
- Banana Cultivation and Research
- Greenhouse Technology and Climate Control
North Carolina State University
2016-2025
North Central State College
2021-2025
Plant (United States)
2021
Cambridge University Press
2021
Chartered Institute of Library and Information Professionals
2018
Universidad de Costa Rica
2017
Clemson University
2017
In-field molecular diagnosis of plant diseases via nucleic acid amplification is currently limited by cumbersome protocols for extracting and isolating pathogenic DNA from tissues. To address this challenge, a rapid extraction method was developed using disposable polymeric microneedle (MN) patch. By applying MN patches on leaves, amplification-assay-ready can be extracted within minute different species. MN-extracted used direct polymerase chain reaction plastid without purification....
Wearable plant sensors hold tremendous potential for smart agriculture. We report a lower leaf surface-attached multimodal wearable sensor continuous monitoring of physiology by tracking both biochemical and biophysical signals the its microenvironment. Sensors detecting volatile organic compounds (VOCs), temperature, humidity are integrated into single platform. The abaxial attachment position is selected on basis stomata density to improve signal strength. This versatile platform enables...
Use of tomato cultivars with the Sw-5 resistance gene cluster has led to occurrence resistance-breaking (RB) spotted wilt virus (TSWV) strains globally, including California and, recently, North Carolina and Texas. We documented disease on infected either an RB strain from (CA-RB) or a wild type (CA-WT) TSWV (cultivar Mountain Merit) without Fresh Plus) Sw-5b detected incidence over time using microneedle RNA extractions LAMP. developed LAMP/Cas12a assay for detection CA-C118Y mutation in...
Phytophthora infestans causes potato late blight, an important and costly disease of tomato crops. Seven clonal lineages P. identified recently in the United States were tested for baseline sensitivity to six oomycete-targeted fungicides. A subset dominant (n = 45) collected between 2004 2012 was vitro on media amended with a range concentrations either azoxystrobin, cyazofamid, cymoxanil, fluopicolide, mandipropamid, or mefenoxam. Dose-response curves values effective concentration at which...
Phytophthora infestans is the causal agent of potato late blight, a devastating disease tomato and threat to global food security. Early detection intervention essential for effective management pathogen. We developed loop-mediated isothermal amplification (LAMP) assay P. compared this conventional PCR, real-time LAMP, droplet digital PCR infestans. The LAMP was specific on did not amplify other potato- or tomato-infecting species fungal bacterial pathogens that infect tomato. threshold SYBR...
Phytophthora infestans (Mont.) de Bary, the causal agent of potato late blight, was responsible for Irish famine 1840s. Initial disease outbreaks occurred in US 1843, two years prior to European outbreaks. We examined evolutionary relationships and source 19th-century using herbarium specimens P. from historic (1846–1970) more recent isolates (1992–2014) pathogen. The same unique SSR multilocus genotype, named here as FAM-1, caused widespread both Europe. FAM-1 lineage shared allelic...
The population structure of the Phytophthora infestans populations that caused recent 2013-14 late blight epidemic in eastern India (EI) and northeastern (NEI) was examined. data provide new baseline information for P. India. A migrant European 13_A2 genotype responsible epidemic, replacing existing populations. Mutations have generated substantial sub-clonal variation with 24 multi-locus genotypes (MLGs) found, which 19 were unique variants not yet reported elsewhere globally. Samples from...
Abstract In 1843, a hitherto unknown plant pathogen entered the US and spread to potato fields in northeast. By 1845, had reached Ireland leading devastating famine. Questions arose immediately about source of outbreaks how disease should be managed. The pathogen, now known as Phytophthora infestans , still continues threaten food security globally. A wealth untapped knowledge exists both archival modern documents, but is not readily available because details are hidden descriptive text....
We examined the evolutionary history of Phytophthora infestans and its close relatives in 1c clade. used whole genome sequence data from 69 isolates species clade conducted a range genomic analyses including nucleotide diversity evaluation, maximum likelihood trees, network assessment, time to most recent common ancestor migration analysis. consistently identified distinct later divergence two Mexican species, P . mirabilis ipomoeae , other species. exhibited more South America, andina...
Summary Crop breeding plays an essential role in addressing food security by enhancing crop yield, disease resistance and nutritional value. However, the current process faces multiple challenges limitations, especially genotypic evaluations. Traditional methods for seed genotyping remain labour‐intensive, time‐consuming cost‐prohibitive outside of large‐scale programs. Here, we present a handheld microneedle (MN)‐based DNA extraction platform rapid, non‐destructive in‐field isolation from...
The FAM-1 genotype of Phytophthora infestans caused late blight in the 1840s US and Europe was responsible for Irish famine. We sampled 140 herbarium specimens collected between 1845 1991 from six continents used 12-plex microsatellite genotyping (SSR) to identify mtDNA lineage (Herb-1/Ia) present historic samples. detected approximately 73% found on continents. US-1 later than all except Australia/Oceania only 27% first almost former British colonies which samples were available. data...
Phytophthora species cause severe diseases on food, forest, and ornamental crops. Since the genus was described in 1876, it has expanded to comprise over 190 formally species. There is a need for an open access phylogenetic tool that centralizes diverse streams of sequence data metadata facilitate research identification We used Tree-Based Alignment Selector Toolkit (T-BAS) develop phylogeny 192 33 informal taxa using sequences eight nuclear genes. The tree inferred RAxML maximum likelihood...
Rapid detection of plant diseases before they escalate can improve disease control. Our team has developed rapid nucleic acid extraction methods with microneedles and combined these loop-mediated amplification (LAMP) assays for pathogen in the field. In this work, we LAMP early blight (
The oomycete Phytophthora infestans is an important plant pathogen on potato and tomato crops. We examined the genetic structure of extant 20th 21st century U.S. lineages P. compared them with populations from South America Mexico to examine relationships potential sources lineages. US-23, currently most prevalent lineage detected in United States, shared similarity primarily BR-1 identified 1990s Bolivia Brazil. Lineages US-8, US-14, US-24, predominantly virulent potato, formed a cluster...
Phytophthora infestans is a major oomycete plant pathogen, responsible for potato late blight, which led to the Irish Potato Famine from 1845–1852. Since then, potatoes resistant this disease have been bred and deployed worldwide. Their resistance (R) genes recognize pathogen effectors virulence then induce response stopping progression. However, most R are quickly overcome by pathogen. We use targeted sequencing of effector on herbarium specimens examine joint evolution in both P....
Abstract Late blight caused by the oomycete Phytophthora infestans is a disease of potato and tomato worldwide relevance widespread throughout Europe Mediterranean region. While pathogen populations in northern have been sampled characterized for many years, genetic structure from southern Europe, including Italy, has less studied. Between 2018 2019, we collected 91 samples P . crops Algeria, Tunisia on FTA cards genotyped them using 12‐plex microsatellites. These were compared to genotypes...
Precision agriculture technology can transform farming related data into useful information, which may lead to more efficient usage of agricultural resources and increase sustainability. This paper compares precision with traditional practices in scheduling fungicide application so as manage late blight disease tomato production. The following three strategies were evaluated: a calendar-based strategy, the BlightPro Decision Support System based strategy (DSS-based strategy), that does not...
Samples from potato fields with lesions late blight-like symptoms were collected eastern North Carolina in 2017 and the causal agent was identified as Phytophthora nicotianae. We have P. nicotianae tomato samples Carolina, Virginia, Maryland, Pennsylvania, New York. Ninety-two field 46 characterized for mefenoxam sensitivity, mating type, simple sequence repeat genotype using microsatellites. Thirty-two percent of isolates A1 while 53% A2 type. In six cases, both types detected same year....
Phytophthora blight, caused by
Two new species of Matelea (Apocynaceae: Asclepiadoideae, Gonolobinae) are described from northern South America: M. fontana and pacifica. The belong to the Ibatia-Matelea complex, but exhibit distinct differences in floral characters previously known taxa, including structure gynostegial coronas. A key descriptions members American West Indian complex is provided, cumanensis, maritima, rubra. Asclepias Cynanchum fimbriatum, Ibatia muricata lectotypified.
Rapid, field-deployable assays such as loop-mediated isothermal amplification (LAMP) are critical for detecting nursery and forest pathogens like Phytophthora ramorum P. kernoviae to prevent pathogen spread. We developed validated four LAMP genus-level detection of spp., species-level lineage-level the NA1 lineage. Cross reactivity two species-specific was evaluated using a set 18 spp. known infect crop hosts. The correct target species were detected by assays. assay against 27 other...
Abstract Crop breeding plays an essential role in addressing food security by enhancing crop yield, disease resistance, and nutritional value. However, the current process faces multiple challenges limitations, especially genotypic evaluations. Traditional methods for seed genotyping remain labor-intensive, time-consuming, cost-prohibitive outside of large-scale programs. Here, we present a handheld microneedle (MN)-based DNA extraction platform rapid, nondestructive, in-field isolation from...