A5074036892- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Plasmonic and Surface Plasmon Research
- Advanced Fluorescence Microscopy Techniques
- Molecular Junctions and Nanostructures
- Gold and Silver Nanoparticles Synthesis and Applications
- Click Chemistry and Applications
- Force Microscopy Techniques and Applications
- Biosensors and Analytical Detection
- Spectroscopy and Quantum Chemical Studies
- Nanofabrication and Lithography Techniques
- Cellular Mechanics and Interactions
- Advanced Electron Microscopy Techniques and Applications
- Quantum-Dot Cellular Automata
- Microfluidic and Bio-sensing Technologies
- SARS-CoV-2 detection and testing
- Near-Field Optical Microscopy
- Photochemistry and Electron Transfer Studies
- Nanopore and Nanochannel Transport Studies
- Porphyrin and Phthalocyanine Chemistry
- Nanowire Synthesis and Applications
- Analytical Chemistry and Sensors
Ludwig-Maximilians-Universität München
2019-2025
Center for NanoScience
2019-2025
Frederick National Laboratory for Cancer Research
2021
University of Würzburg
2021
National Cancer Institute
2021
Center for Cancer Research
2021
National Heart Lung and Blood Institute
2021
University of Göttingen
2020
The advent of highly sensitive photodetectors and the development photostabilization strategies made detecting fluorescence single molecules a routine task in many labs around world. However, to this day, process requires cost-intensive optical instruments due truly nanoscopic signal emitter. Simplifying single-molecule detection would enable exciting applications, e.g., point-of-care diagnostic settings, where costly equipment be prohibitive. Here, we introduce addressable NanoAntennas with...
Appending conformationally restraining ring systems to the cyanine chromophore creates exceptionally bright fluorophores in visible range. Here, we report application of this strategy near-infrared range through preparation first restrained heptamethine indocyanine. Time-resolved absorption spectroscopy and fluorescence correlation verify that, unlike corresponding parent unrestrained variant, molecule is not subject photoisomerization. Notably, however, room-temperature emission efficiency...
A bottleneck in many studies utilizing single-molecule Förster resonance energy transfer is the attainable photon count rate, as it determines temporal resolution of experiment. As biologically relevant processes occur on time scales that are hardly accessible with currently achievable rates, there has been considerable effort to find strategies increase stability and brightness fluorescent dyes. Here, we use DNA nanoantennas drastically rates observe fast biomolecular dynamics small volume...
ConspectusThe possibility to increase fluorescence by plasmonic effects in the near-field of metal nanostructures was recognized more than half a century ago. A major challenge, however, use this effect because placing single quantum emitters nanoscale hotspot remained unsolved for long time. This not only presents chemical problem but also requires nanostructure itself be coaligned with polarization excitation light. Additional difficulties arise from complex distance dependence emission:...
Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications, but their photon output can be limited by trans-to-cis photoisomerization. We recently demonstrated that appending ring system to the pentamethine cyanine improves quantum yield and extends fluorescence lifetime. Here, we report an optimized synthesis persulfonated variants enable efficient labeling nucleic acids proteins. demonstrate bifunctional sulfonated tertiary amide significantly optical...
Abstract Photobleaching of fluorescence labels poses a major limitation in single-molecule and super-resolution microscopy. Conventional photostabilization methods, such as oxygen removal addition high concentrations additives, often require careful fluorophore selection can disrupt the biological environment. To address these limitations, we developed modular minimally invasive approach that utilizes DNA-mediated delivery photostabilizer directly to imaging site. Under lower excitation...
Abstract Fluorescent dyes used for single-molecule spectroscopy can undergo millions of excitation-emission cycles before photobleaching. Due to the upconcentration light in a plasmonic hotspot, conditions fluorescent are even more demanding DNA origami nanoantennas. Here, we briefly review current state fluorophore stabilization imaging and reveal additional factors relevant context fluorescence enhancement. We show that despite improved photostability fluorophores by nanoantennas, their...
Most measurements of fluorescence lifetimes on the single-molecule level are carried out using avalanche photon diodes (APDs). These single-photon counters inherently slow, and their response shows a strong dependence energy, which can make reconvolution instrument function (IRF) challenging. An ultrafast time resolution in is crucial, e.g., determining donor donor–acceptor couples undergo energy transfer, or plasmonic antenna structures, where radiative rate non-radiative rates enhanced. We...
Cell cortices are responsible for the resilience and morphological dynamics of cells. Measuring their mechanical properties is impeded by contributions from other filament types, organelles, crowded cytoplasm. We established a versatile concept precise assessment cortical viscoelasticity based on force cycle experiments paired with continuum mechanics. Apical cell membranes confluent MDCK II cells were deposited porous substrates locally deformed. Force cycles could be described...
Abstract DNA nanotechnology has conquered the challenge of positioning quantum emitters in hotspot optical antenna structures for fluorescence enhancement. Therefore, origami serves as scaffold to arrange nanoparticles and emitters, such fluorescent dyes. For next optimizing applicability plasmonic hotspots molecular assays, a Trident structure that increases accessibility is introduced, thereby improving kinetics target molecule binding. This NanoAntenna with Cleared HOtSpot (NACHOS)...
Abstract Biosensors play key roles in medical research and diagnostics, but there currently is a lack of sensing platforms that combine easy adaptation to new targets, strategies tune the response window relevant analyte concentration ranges allow for incorporation multiple elements benefit from multivalency. Utilizing DNA origami nanostructure as scaffold arranging different sensor components, we here propose an approach development modular tunable single-molecule sensors capable detecting...
Abstract Cyanine dyes are exceptionally useful probes for a range of fluorescence‐based applications, but their photon output can be limited by trans ‐to‐ cis photoisomerization. We recently demonstrated that appending ring system to the pentamethine cyanine improves quantum yield and extends fluorescence lifetime. Here, we report an optimized synthesis persulfonated variants enable efficient labeling nucleic acids proteins. demonstrate bifunctional sulfonated tertiary amide significantly...
The advent of highly sensitive photodetectors 1,2 and the development photostabilization strategies 3 made detecting fluorescence a single molecule routine task in many labs around world. However, to this day, process requires cost-intensive optical instruments due truly nanoscopic signal emitter. Simplifying single-molecule detection would enable exciting applications, e.g. point-of-care diagnostic settings, where costly equipment be prohibitive. 4 Here, we introduce addressable...
In recent years, DNA nanotechnology has matured to enable robust production of complex nanostructures and hybrid materials. We have combined with sensitive optical detection create functional single-molecule devices that new applications in biosensing superresolution microscopy. Starting nanorulers brightness reference samples we determined the resolving power microscopes evaluated sensitivity smartphone cameras. To improve created origami antennas for metal enhanced fluorescence. The unique...
Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications. We recently demonstrated that appending ring system to the pentamethine cyanine improves quantum yield and extends fluorescence lifetime. Here, we report an optimized synthesis persulfonated variants enable efficient labeling nucleic acids proteins. demonstrate bifunctional sulfonated tertiary amide significantly optical properties resulting bioconjugates. These new conformationally restricted...
Abstract A bottleneck in many studies utilizing single-molecule Förster Resonance Energy Transfer (smFRET) is the attainable photon count rate as it determines temporal resolution of experiment. As biologically relevant processes occur on timescales that are hardly accessible with currently achievable rates, there has been considerable effort to find strategies increase stability and brightness fluorescent dyes. Here, we use DNA nanoantennas drastically rates observe fast biomolecular...
Trident DNA Origami In article number 2200255, Cindy Close, Philip Tinnefeld, and co-workers demonstrate the development of a origami structure for single-molecule-based plasmonic biosensing. NanoAntennas with Cleared HOtSpots (NACHOS) are compared previous nanoantenna designs improved hotspot accessibility is demonstrated. Adjusting size cleared region improves kinetics target molecule binding without compromising fluorescence enhancement.
Cyanine dyes are exceptionally useful probes for a range of fluorescence-based applications. We recently demonstrated that appending ring system to the pentamethine cyanine improves quantum yield and extends fluorescence lifetime. Here, we report an optimized synthesis persulfonated variants enable efficient labeling nucleic acids proteins. demonstrate bifunctional sulfonated tertiary amide significantly optical properties resulting bioconjugates. These new conformationally restricted...