A5006987122- Advanced biosensing and bioanalysis techniques
- Plasmonic and Surface Plasmon Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Biosensors and Analytical Detection
- Molecular Junctions and Nanostructures
- RNA Interference and Gene Delivery
- Graphene research and applications
- SARS-CoV-2 detection and testing
- Surgical Sutures and Adhesives
- Dental Implant Techniques and Outcomes
- Polymer Surface Interaction Studies
- Nanocluster Synthesis and Applications
- Analytical Chemistry and Sensors
- Orbital Angular Momentum in Optics
- Advanced Polymer Synthesis and Characterization
- Medical and Biological Sciences
- Nanowire Synthesis and Applications
- dental development and anomalies
- Oral Health Pathology and Treatment
- Dental Radiography and Imaging
- Surfactants and Colloidal Systems
- Protein Interaction Studies and Fluorescence Analysis
- Oral and gingival health research
Center for NanoScience
2019-2024
Ludwig-Maximilians-Universität München
2019-2024
University of Duisburg-Essen
2014-2019
The advent of highly sensitive photodetectors and the development photostabilization strategies made detecting fluorescence single molecules a routine task in many labs around world. However, to this day, process requires cost-intensive optical instruments due truly nanoscopic signal emitter. Simplifying single-molecule detection would enable exciting applications, e.g., point-of-care diagnostic settings, where costly equipment be prohibitive. Here, we introduce addressable NanoAntennas with...
Abstract Ideal dimers comprising gold nanoparticles with a smooth surface and high sphericity are synthesized by substrate‐based assembly strategy efficient cetyltrimethylammonium bromide removal. An unprecedented structural plasmonic uniformity at the single‐particle level is observed since inhomogeneities resulting from variations in gap morphology eliminated. Single ideal analyzed polarization‐resolved dark‐field scattering spectroscopy. Contributions transverse as well quadrupolar...
The surface plasmon (SP) coupling in dimers of spherical and faceted gold nanoparticles was investigated experimentally computationally at the single-particle level. Individual ideal two with a constant gap, filled by self-assembled monolayer 1,8-octanedithiol linker molecules, exhibit highly uniform dark-field (DF) scattering spectra. In contrast, single nonideal same gap high degree spectral nonuniformity. We attribute this significant heterogeneity to many possible morphologies, that is,...
Despite the thorough investigation of graphene since 2004, altering its surface chemistry and reproducible functionalization remain challenging. This hinders fabrication more complex hybrid materials with controlled architectures, as a consequence development sensitive reliable sensors biological assays. In this contribution, we introduce DNA origami structures nanopositioners for placing single dye molecules at distances from graphene. The measurements fluorescence intensity lifetime...
We demonstrate the capability of DNA self-assembled optical antennas to direct emission an individual fluorophore, which is free rotate. origami used fabricate composed two colloidal gold nanoparticles separated by a predefined gap and place single Cy5 fluorophore near center. Although able rotate, its excitation far-field mediated antenna, with directionality following dipolar pattern according antenna main resonant mode. This work intended set out basis for manipulating molecules based on...
The DNA origami technique itself is considered a milestone of nanotechnology and nanorulers represent the first widespread application this technique. are used to demonstrate capabilities techniques valuable training samples. They have meanwhile been developed for multitude microscopy methods including optical microscopy, atomic force electron their unique properties further exploited develop point-light sources, brightness references, nanophotonic test structures, alignment tools...
Graphene exhibits outstanding fluorescence quenching properties that can become useful for biophysics and biosensing applications, but it remains challenging to harness these advantages due the complex transfer procedure of chemical vapor deposition-grown graphene glass coverslips low yield usable samples. Here, we screen 10 graphene-on-glass preparation methods present an optimized protocol. To obtain required quality single-molecule super-resolution imaging on graphene, introduce a...
Abstract DNA self‐assembly is a powerful tool to arrange optically active components with high accuracy in large parallel manner. A facile approach assemble plasmonic antennas consisting of two metallic nanoparticles (40 nm) single colloidal quantum dot positioned at the hot spot presented here. The design based on complementarity, stoichiometry, and steric hindrance principles. Since no intermediate molecules other than short strands are required, structures possess very small gap (≈ 5...
Single-stranded DNA on MNPs shows a transition from being coiled to brushed out at low and high grafting densities, respectively. Thereby, exhibit cooperative dynamics, which is highly relevant for magnetic biosensing binding origami.
The permeability of the polymer walls polyalkylcyanoacrylate nanocapsules varies by different degrees chemical cross-linking. For this reason, amounts bivalent alkylcyanoacrylates are added to monovalent alkylcyanoacrylate prior an interfacial polymerization step in order generate capsules with various cross-linking densities. obtained characterized observing water molecules via pulsed field-gradient nuclear magnetic resonance using a stimulated echo sequence. resulting decay plots reveal...
Abstract Biosensors play key roles in medical research and diagnostics, but there currently is a lack of sensing platforms that combine easy adaptation to new targets, strategies tune the response window relevant analyte concentration ranges allow for incorporation multiple elements benefit from multivalency. Utilizing DNA origami nanostructure as scaffold arranging different sensor components, we here propose an approach development modular tunable single-molecule sensors capable detecting...
The advent of highly sensitive photodetectors 1,2 and the development photostabilization strategies 3 made detecting fluorescence a single molecule routine task in many labs around world. However, to this day, process requires cost-intensive optical instruments due truly nanoscopic signal emitter. Simplifying single-molecule detection would enable exciting applications, e.g. point-of-care diagnostic settings, where costly equipment be prohibitive. 4 Here, we introduce addressable...
In article number 1702754, Sebastian Schlücker and co-workers synthesize ideal gold dimers of two spherical nanoparticles with a controlled gap distance morphology. While theoreticians have been able to describe the properties such model system, experimentalists failed it. Here, elastic scattering an dimer using white light together electron microscopy image is shown. The polar plot summarizes polarization behavior various higher order plasmon coupling modes.
ABSTRACT Magnetic nanoparticles (MNPs) enable unique capabilities for biosensing and actuation via coupling to DNA origami, yet how grafting density affects their dynamics accessibility remains poorly understood. Here, we demonstrate functionalization of MNPs with single-stranded (ssDNA) click chemistry conjugation tunable density. Several complementary methods show that particle translational rotational exhibit a sigmoidal dependence on ssDNA At low densities strands are coiled cause small...