A5050207518- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Digital Holography and Microscopy
- Innovative Microfluidic and Catalytic Techniques Innovation
- Characterization and Applications of Magnetic Nanoparticles
- 3D Printing in Biomedical Research
- Pain Management and Opioid Use
- Proteoglycans and glycosaminoglycans research
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Sensor and Energy Harvesting Materials
- Microfluidic and Capillary Electrophoresis Applications
- Soil Moisture and Remote Sensing
- Electrowetting and Microfluidic Technologies
- Corneal Surgery and Treatments
- Pain Mechanisms and Treatments
- Advanced Biosensing Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Nanoplatforms for cancer theranostics
- Epigenetics and DNA Methylation
- Advancements in Transdermal Drug Delivery
- Advanced Materials and Mechanics
- Inhalation and Respiratory Drug Delivery
- Cell Image Analysis Techniques
- Mobile Health and mHealth Applications
Samueli Institute
2021
University of California, Los Angeles
2014-2021
California NanoSystems Institute
2014-2017
UCLA Jonsson Comprehensive Cancer Center
2014-2015
Los Angeles City College
2014-2015
Key challenges with point-of-care (POC) nucleic acid tests include achieving a low-cost, portable form factor, and stable readout, while also retaining the same robust standards of benchtop lab-based tests. We addressed two crucial aspects this problem, identifying chemical additive, hydroxynaphthol blue, that both stabilizes significantly enhances intercalator-based fluorescence readout concentration, developing cost-effective fiber-optic bundle-based microplate reader integrated onto...
Nucleic acid amplification assays including loop-mediated isothermal (LAMP) are routinely used in diagnosing diseases and monitoring water food quality. The results of these commonly measured with an analog fluorescence readout, which requires specialized optical equipment can lack quantitative precision. Digital analysis small fluid compartments based on exceeding a threshold level enhance the precision nucleic (i.e., digital assays), but still systems for readout inclusion fluorescent dye....
The emergence of epigenetic gene regulation and its role in disease have motivated a growing field diagnostics for risk assessment screening. In particular, irregular cytosine DNA base methylation has been implicated several diseases, yet the methods detecting these marks are limited to lengthy protocols requiring bulky costly equipment. We demonstrate simple workflow methylated CpG dinucleotides synthetic genomic samples using methylation-sensitive restriction enzyme digestion followed by...
Abstract Detecting rare cells within blood has numerous applications in disease diagnostics. Existing cell detection techniques are typically hindered by their high cost and low throughput. Here, we present a computational cytometer based on magnetically modulated lensless speckle imaging, which introduces oscillatory motion to the magnetic-bead-conjugated of interest through periodic magnetic force uses time-resolved holographic imaging rapidly detect target three dimensions (3D). In...
A process to surface pattern polydimethylsiloxane (PDMS) with ferromagnetic structures of varying sizes (micrometer millimeter) and thicknesses (>70 μm) is developed. Their flexibility magnetic reach are utilized confer dynamic, additive properties a variety substrates, such as coverslips Eppendorf tubes. It found that these substrates can generate additional modes droplet manipulation, tunably steer magnetic-cell organization. As service our authors readers, this journal provides supporting...
The ability to break up a volume of fluid into smaller pieces that are confined or separated prevent molecular communication/transport is key capability intrinsic microfluidic systems. This has been used develop implement digital versions traditional analysis assays, including PCR and immunoassays/ELISA. In these versions, the concentration target analyte in range such that, when sampled volumes, either single molecule no may be present. Subsequent amplification sensitive enough obtain...
We present an approach to estimate the concentration of a biomolecule in solution by sampling several nanoliter-scale volumes and determining if contain any biomolecules. In this method, varying volume fractions (nanoliter-scale) sample nucleic acids are introduced array uniform reaction wells (100 μL), which then fluorescently imaged determine signal is above threshold after acid amplification, all without complex instrumentation. The nanoliter generated using simple positioning permanent...
Microfluidic and microfabricated systems are providing key functionalities in diagnostic therapeutic scenarios, translating beyond the research laboratory to pre-clinical animal studies clinical with patients. Here, we highlight a recent study making use of miniaturization automation development smartphone-integrated point-of-care detect antibodies infectious diseases global health setting. We also review an intraocular implanted system for glaucoma that relies on imaging location fluid...
Lab on a chip systems have often focused diagnostic, chemical, and cell analysis applications, however, more recently the scale and/or precision of micro-engineered has been applied in developing new therapies. In this issue we highlight recent work using microfluidic therapeutic applications. We discuss two approaches that use to address challenges filtering blood--to both remove unwanted pathogens toxins isolate rare cells interest potential. cases chemically-modified surfaces,...
We present a cost-effective and high-throughput computational cytometer using magnetically-modulated lensless imaging technique deep learning-based classification, to rapidly detect rare cells in whole blood, achieving detection limit of 10 cells/mL.
We present a high-throughput and cost-effective computational cytometer for rare cell detection, where the target cells are specifically labeled with magnetic particles exhibit an oscillatory motion under periodically-changing field. The time-varying diffraction patterns of oscillating then captured holographic imaging system further classified by customized pseudo-3D convolutional network. To evaluate performance our technique, we detected serially-diluted MCF7 cancer that were spiked in...
We present a deep learning-based high-throughput cytometer to detect rare cells in whole blood using cost-effective and light-weight design. This system uses magnetic-particles label enrich the target cells. Then, periodically-alternating magnetic-field creates time-modulated diffraction patterns of that are recorded lensless microscope. Finally, custom-designed convolutional network is used classify based on their modulated spatio-temporal patterns. was tested with cancer spiked achieve...