A5045121521- Renal and related cancers
- Complex Network Analysis Techniques
- Opinion Dynamics and Social Influence
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Renal cell carcinoma treatment
- Theoretical and Computational Physics
- Gene Regulatory Network Analysis
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Cell Image Analysis Techniques
- Chaos control and synchronization
- RNA modifications and cancer
- Computational Drug Discovery Methods
- Complex Systems and Time Series Analysis
- Evolution and Genetic Dynamics
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Genetic factors in colorectal cancer
- Eosinophilic Disorders and Syndromes
- Organ Donation and Transplantation
Bar-Ilan University
2016-2025
Stanford University
2007-2020
Institute of Nanotechnology
2019
University of California, San Diego
2014
Howard Hughes Medical Institute
2009-2014
Bioengineering Center
2012
GeoEngineers (United States)
2012
Weizmann Institute of Science
2007-2009
The identification of high-risk stage II colon cancers is key to the selection patients who require adjuvant treatment after surgery. Microarray-based multigene-expression signatures derived from stem cells and progenitor hold promise, but they are difficult use in clinical practice.
“Bulk” measurements of antiviral innate immune responses from pooled cells yield averaged signals and do not reveal underlying signaling heterogeneity in infected bystander single cells. We examined such the small intestine during rotavirus (RV) infection. Murine RV EW robustly activated type I IFNs several genes (IFN-stimulated genes) by bulk analysis, source induced primarily being hematopoietic Flow cytometry microfluidics-based single-cell multiplex RT-PCR allowed dissection IFN...
There is a revolution in the ability to analyze gene expression of single cells tissue. To understand this data we must comprehend how are distributed high-dimensional space. One open question whether cell types form discrete clusters or forms continuum states. If such exists, what its geometry? Recent theory on evolutionary trade-offs suggests that need perform multiple tasks arranged polygon polyhedron (line, triangle, tetrahedron and so on, generally called polytopes) space, whose...
The origins and functions of kidney macrophages in the adult have been explored, but their roles during development remain largely unknown. Here we characterise macrophage arrival, localisation, heterogeneity, organogenesis. Using genetic approaches to ablate macrophages, identify a role for nephron progenitor cell clearance as mouse begins. Throughout renal organogenesis, most are perivascular express F4/80 CD206. These enriched mRNAs linked developmental processes, such blood vessel...
Cells respond to the environment by regulating expression of genes according environmental signals. The relation between input signal level and gene is called regulation function. It interest understand shape a function in terms which it has evolved basic constraints biological systems. Here we address this presenting cost–benefit theory for functions that takes into account temporally varying inputs stochastic noise components. We apply well-studied lac operon E. coli. present explains...
An incomplete understanding of the nature heterogeneity within stem cell populations remains a major impediment to development clinically effective cell-based therapies. Transcriptional events single are inherently stochastic and can produce tremendous variability, even among genetically identical cells. It unclear how mammalian cellular systems overcome this intrinsic noisiness gene expression consequential variations in function, what impact has on biologic clinical relevance highly...
Previous studies have proposed that epithelial to mesenchymal transition (EMT) in breast cancer cells regulates metastasis, stem cell properties and chemo-resistance; most were based on vitro culture of lines mouse transgenic models. However, the identity function expressing EMT-associated genes normal murine mammary gland homeostasis human still remains under debate. Using vivo lineage tracing triple negative (TNBC) patient derived xenografts we demonstrate repopulating capacity tumorigenic...
The stem cell niche is a complex local signaling microenvironment that sustains activity during organ maintenance and regeneration. mammary gland must support its associated cells while also responding to systemic hormonal regulation triggers pubertal changes. We find Gli2, the major Hedgehog pathway transcriptional effector, acts within mouse stromal direct hormone-responsive program by activating expression of factors regulate epithelial as well receptors for mammatrophic hormones estrogen...
Abstract When assembling a nephron during development multipotent stem cell pool becomes restricted as differentiation ensues. A faulty arrest in this process leads to transformation and initiation of Wilms’ tumor. Mapping these transitions with respective surface markers affords accessibility specific subpopulations. NCAM1 CD133 have been previously suggested mark human renal progenitor populations. Herein, using sorting, RNA sequencing, vitro studies serum-free media vivo...
We review results on the scaling of optimal path length in random networks with weighted links or nodes. In strong disorder we find that increases dramatically compared to known small world result for minimum distance. For Erd\H{o}s-R\'enyi (ER) and scale free (SF), parameter $\lambda$ ($\lambda >3$), small-world nature is destroyed. also numerically weak scales logaritmically size studied. transition between regimes properties ER SF a general distribution weights, suggest any weigths,...
A current challenge in biology is to understand the dynamics of protein circuits living human cells. Can one define and test equations for variability a over time? Here, we address this experimentally theoretically, by means accurate time-resolved measurements endogenously tagged proteins individual As model system, choose three stable displaying cell-cycle–dependant dynamics. We find that accumulation with time per cell quadratic long mRNA life times approximately linear short lifetime....
End-stage renal disease is a worldwide epidemic requiring replacement therapy. Harvesting tissue from failing kidneys and autotransplantation of progenitors could theoretically delay the need for dialysis. Here we use healthy end-stage human adult to robustly expand proliferative kidney epithelial cells establish 3D cultures termed "nephrospheres." Formation nephrospheres reestablishes identity function in primary cultures. Transplantation into NOD/SCID mice shows that restore...