A5041903342- Zebrafish Biomedical Research Applications
- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Epigenetics and DNA Methylation
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Immune cells in cancer
- Pregnancy and preeclampsia studies
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Neonatal Respiratory Health Research
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Virus-based gene therapy research
- Renal and related cancers
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Cancer, Hypoxia, and Metabolism
- Reproductive System and Pregnancy
- Angiogenesis and VEGF in Cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Hemoglobinopathies and Related Disorders
University of Edinburgh
2015-2024
Centre for Inflammation Research
2015-2024
MRC Centre for Regenerative Medicine
2016-2024
Southern Medical University
2023
The Queen's Medical Research Institute
2015-2023
Jinan University
2023
Edinburgh College
2020
Queen's Medical Centre
2017-2019
Peking University People's Hospital
2019
University of California, Los Angeles
2019
Abstract Vascular smooth muscle cells (VSMCs) show pronounced heterogeneity across and within vascular beds, with direct implications for their function in injury response atherosclerosis. Here we combine single-cell transcriptomics lineage tracing to examine VSMC healthy mouse vessels. The transcriptional profiles of single VSMCs consistently reflect region-specific developmental history heterogeneous expression disease-associated genes involved inflammation, adhesion migration. We detect a...
Hematopoietic cell clusters in the aorta of vertebrate embryos play a pivotal role formation adult blood system. Despite their importance, hematopoietic have not been systematically quantitated or mapped because technical limitations posed by opaqueness whole mouse embryos. Here, we combine an approach to make transparent, with multicolor marking, allow observation using high-resolution 3-dimensional confocal microscopy. Our method provides first complete map and temporal quantitation all...
Identifying signaling pathways that regulate hematopoietic stem and progenitor cell (HSPC) formation in the embryo will guide efforts to produce expand HSPCs ex vivo. Here we show sterile tonic inflammatory regulates embryonic HSPC formation. Expression profiling of progenitors with lymphoid potential cells (HSCs) from aorta/gonad/mesonephros (AGM) regions midgestation mouse embryos revealed a robust innate immune/inflammatory signature. Mouse lacking interferon γ (IFN-γ) or IFN-α zebrafish...
Knowledge of the key transcription factors that drive hematopoietic stem cell (HSC) generation is particular importance for current regenerative approaches and reprogramming strategies. Whereas GATA2 has long been implicated as a factor its dysregulated expression associated with human immunodeficiency syndromes vascular integrity, it yet unknown how functions in HSCs. HSCs are generated from endothelial cells major embryonic vasculature (aorta, vitelline, umbilical arteries) found...
GATA-2 is an essential transcription factor in the hematopoietic system that expressed stem cells (HSCs) and progenitors. Complete deficiency of mouse leads to severe anemia embryonic lethality. The role dosage effects this HSC development within embryo adult are largely unexplored. Here we examined gene on generation expansion HSCs several sites throughout development. We show a haploid dose severely reduces production specifically aorta-gonad-mesonephros region (which autonomously...