A5013014400- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Effects of Radiation Exposure
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Nuclear Structure and Function
- MicroRNA in disease regulation
- Photoacoustic and Ultrasonic Imaging
- Cancer Immunotherapy and Biomarkers
- Digital Holography and Microscopy
- Gut microbiota and health
- Advanced Fluorescence Microscopy Techniques
- Protein Degradation and Inhibitors
- Multiple Myeloma Research and Treatments
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Telomeres, Telomerase, and Senescence
- PARP inhibition in cancer therapy
Westlake University
2019-2025
Fujian Electric Power Survey & Design Institute
2024
Soochow University
2022
California Institute for Regenerative Medicine
2012-2022
Stanford University
2013-2022
Second Affiliated Hospital of Soochow University
2020
Soochow University
2020
Philips (Netherlands)
2018
Shenyang Jianzhu University
2017
Institute for Stem Cell Biology and Regenerative Medicine
2014
More diversity at the top A detailed knowledge of cell differentiation hierarchies is important for understanding diverse biological processes such as organ development, tissue regeneration, and cancer. Single-cell RNA sequencing can help elucidate these hierarchies, but it requires reliable computational methods predicting lineage trajectories. Gulati et al. developed CytoTRACE, a framework based on simple observation that transcriptional diversity—the number genes expressed in...
Kinesin-14 family proteins are minus-end directed motors that cross-link microtubules and play key roles during spindle assembly. We showed previously the Xenopus XCTK2 is regulated by Ran via association of a bipartite NLS in tail with importin alpha/beta, which regulates its ability to formation. Here we show mutation nuclear localization signal (NLS) human HSET caused an accumulation cytoplasm, resulted strong microtubule bundling. overexpression HeLa cells longer spindles, similar what...
MicroRNAs (miRNAs) are important regulators of stem and progenitor cell functions. We previously reported that miR-142 miR-150 upregulated in human breast cancer cells (BCSCs) as compared to the non-tumorigenic cells. In this study, we report efficiently recruits APC mRNA an RNA-induced silencing complex, activates canonical WNT signaling pathway APC-suppression dependent manner, expression miR-150. Enforced or normal mouse mammary resulted regeneration hyperproliferative glands vivo....
Normal stem cells from a variety of tissues display unique metabolic properties compared to their more differentiated progeny. However, relatively little is known about cancer cells, also called tumor initiating (TICs). In this study we show that, analogous some normal breast TICs have distinct nontumorigenic (NTCs). Transcriptome profiling using RNA-Seq revealed underexpress genes involved in mitochondrial biology and oxidative phosphorylation, analyses preferentially perform glycolysis...
We present a tiling light sheet microscope compatible with all tissue clearing methods for rapid multicolor 3D imaging of cleared tissues micron-scale (4 × 4 10 μm3) to submicron-scale (0.3 0.3 1 spatial resolution. The resolving ability is improved sub-100 nm (70 70 200 nm3) via expansion. uses sheets achieve higher resolution and better optical sectioning than conventional microscopes. illumination phase modulated adjust the position intensity profile based on desired speed keep aligned....
Abstract Spatially resolved proteomics is an emerging approach for mapping proteome heterogeneity of biological samples, however, it remains technically challenging due to the complexity tissue microsampling techniques and mass spectrometry analysis nanoscale specimen volumes. Here, we describe a spatially method based on combination expansion with spectrometry-based proteomics, which call Expansion Proteomics (ProteomEx). ProteomEx enables quantitative profiling spatial variability in...
Cancer incidence escalates exponentially with advancing age; however, the underlying mechanism remains unclear. In this study, we build a chronological molecular clock at single-cell transcription level mammary stem cell-enriched population to depict physiological aging dynamics in female mice. We find that process is asynchronous and progressive, initiated by an early senescence program, succeeded entropic late program elevated cancer associated pathways, vulnerable predisposition. The...
Lamin B receptor (LBR), a chromatin and lamin binding protein in the inner nuclear membrane, has been proposed to play vital role envelope (NE) assembly. But specific for LBR NE assembly remains unknown. In present study, we show that overexpression of causes membrane overproduction, inducing invagination stack formation, these processes require transmembrane domain LBR. Biochemical analysis shows N-terminal directly interacts with importin β Ran sensitive α independent manner. Using an...
Previous studies have proposed that epithelial to mesenchymal transition (EMT) in breast cancer cells regulates metastasis, stem cell properties and chemo-resistance; most were based on vitro culture of lines mouse transgenic models. However, the identity function expressing EMT-associated genes normal murine mammary gland homeostasis human still remains under debate. Using vivo lineage tracing triple negative (TNBC) patient derived xenografts we demonstrate repopulating capacity tumorigenic...
The stem cell niche is a complex local signaling microenvironment that sustains activity during organ maintenance and regeneration. mammary gland must support its associated cells while also responding to systemic hormonal regulation triggers pubertal changes. We find Gli2, the major Hedgehog pathway transcriptional effector, acts within mouse stromal direct hormone-responsive program by activating expression of factors regulate epithelial as well receptors for mammatrophic hormones estrogen...
The BRCA1/BARD1 complex plays a key role in the repair of DNA double-strand breaks (DSBs) both somatic cells and germ cells. However, underlying molecular mechanism by which this mediates DSB is not fully understood. Here, we examined XY body male cells, where DSBs are accumulated. We show that recruitment to unsynapsed axis mediated pre-ribosomal RNA (pre-rRNA). Similarly, associates with pre-rRNA only forms nuclear foci response DSBs, but also targets DSBs. interactions between BRCT...
// Zheng-wei Li 1, * , Shang Cai 2, Yuanyuan Liu 3, Chuan-lai Yang Ye Tian 2 Gang Chen 4 C. Cao 3 1 Surgical Department I, Xuzhou Children's Hospital, Xuzhou, China of Radiotherapy and Oncology, the Second Affiliated Hospital Soochow University, Suzhou, Institute Neuroscience, Neurosurgery, First Jiangsu, Co-first authors Correspondence to: Tian, email: tianyesuzhoua163@163.com dryetian@126.com Chen, nju_neurosurgery@163.com Cao, caocong@suda.edu.cn Keywords: glioma, gαi3, receptor...
Abstract Background: The development of chemoresistance in many cancer patients poses a significant clinical challenge, while the underlying causes for heterogeneous drug responses remain largely elusive. Increasing evidence suggests that tumors exhibit extensive heterogeneity and harbor subpopulations less differentiated states are linked to treatment resistance. Therefore, dissecting immature cell pre-existing is essential identifying biomarkers associated with establishing targeted...