A5032868413- HIV Research and Treatment
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Viral Infections and Outbreaks Research
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Viral Infections and Vectors
- Viral Infectious Diseases and Gene Expression in Insects
- HIV/AIDS Research and Interventions
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Virus Infections Studies
- interferon and immune responses
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Pluripotent Stem Cells Research
University of Southern California
2014-2024
Southern California University for Professional Studies
2001-2023
Children's Hospital of Los Angeles
2005-2009
Washington State University
1998
USC Norris Comprehensive Cancer Center
1997-1998
Gene Therapy Laboratory
1998
University of Oxford
1994-1997
Science Oxford
1996
University of Liverpool
1992
Western University
1992
Journal Article A transient three-plasmid expression system for the production of high titer retroviral vectors Get access Yuko Soneoka, Soneoka Retrovirus Molecular Biology Group, Department Biochemistry, University OxfordSouth Parks Road, Oxford 0X1 3QU, UK Search other works by this author on: Academic PubMed Google Scholar Paula M. Cannon, Cannon Emma E. Ramsdale, Ramsdale Joanne C. Griffiths, Griffiths Gaetano Romano, Romano Susan Kingsman, Kingsman Alan J. * To whom correspondence...
The HIV-1 coreceptor CCR5 is a validated target for HIV/AIDS therapy. apparent elimination of in patient treated with an allogeneic stem cell transplant homozygous naturally occurring deletion mutation (CCR5Δ32/Δ32) supports the concept that single dose HIV-resistant hematopoietic cells can provide disease protection. Given low frequency CCR5Δ32/Δ32 donors, we reasoned engineered autologous CD34+ stem/progenitor (HSPCs) could be used AIDS We evaluated disruption gene expression HSPCs...
Abstract Background In the absence of Vpu protein, newly formed HIV-1 particles can remain attached to surface human cells due action an interferon-inducible cellular restriction factor, BST-2/tetherin. Tetherin also restricts release other enveloped viral and is counteracted by a several anti-tetherin factors including HIV-2 Env, SIV Nef KSHV K5 proteins. Results We observed that fraction tetherin located at restricting cells, co-expression both Env reduced this population. addition, Vpu,...
Gene therapy for HIV-1 infection is a promising alternative to lifelong combination antiviral drug treatment. Chemokine receptor 5 (CCR5) the coreceptor required R5-tropic of human cells. Deletion CCR5 renders cells resistant infection, and potential cure has been shown through allogeneic stem cell transplantation with naturally occurring homozygous deletion in donor hematopoietic stem/progenitor (HSPC). The requirement HLA-matched HSPC bearing deletions prohibits widespread application this...
Recently developed genomic editing technologies have the potential to be powerful tools for gene therapy because of their ability inactivate genes, correct mutated sequences, or insert intact genes. While field is advancing at an exceptionally rapid pace, there remain key issues regarding development appropriate preclinical assays evaluate off-target effects and establish safety. In order begin a dialogue on these issues, National Institutes Health (NIH) Office Science Policy, in...
We have developed a method to introduce novel paratopes into the human antibody repertoire by modifying immunoglobulin (Ig) genes of mature B cells directly using genome editing technologies. used CRISPR-Cas9 in homology directed repair strategy, replace heavy chain (HC) variable region cell lines with that from an HIV broadly neutralizing (bnAb), PG9. Our strategy is designed function undergone VDJ recombination any combination (V), diversity (D) and joining (J) genes. The modified locus...
Arenaviruses are rodent-borne viruses, with five members of the family capable causing severe hemorrhagic fevers if transmitted to humans. To date, two distinct cellular receptors have been identified that used by different pathogenic alpha-dystroglycan Lassa fever virus and transferrin receptor 1 (TfR1) certain New World clade B viruses. Our previous studies suggested other, as-yet-unknown involved in arenavirus entry. In present study, we examined use TfR1 glycoproteins (GPs) from a panel...
BST-2/tetherin is an interferon-inducible protein that restricts the release of enveloped viruses from surface infected cells by physically linking viral and cellular membranes. It present at both cell in a perinuclear region, anti-tetherin factors including HIV-1 Vpu HIV-2 Env have been shown to decrease population. To map domains human tetherin necessary for virus restriction sensitivity factors, we constructed series derivatives assayed their activity. We found cytoplasmic tail (CT)...
Sequence comparisons of the integrase (IN) proteins from different retroviruses have identified several highly conserved residues. We introduced mutations at 16 these sites into gene human immunodeficiency virus type 1 and analyzed phenotypes resulting viruses. The viruses were all normal for p24 content reverse transcriptase activity. In addition, mutants could infect T-cell lines undergo transcription, as assessed by PCR analysis. Most mutant also had Western blot (immunoblot) profiles,...
ABSTRACT Pseudotyping retrovirus and lentivirus vectors with different viral fusion proteins is a useful strategy to alter the host range of vectors. Although are efficiently pseudotyped by Env from several subtypes murine leukemia virus (MuLV), related protein gibbon ape (GaLV) does not form functional pseudotypes. We have determined that this arises because an inability GaLV be incorporated into vector particles. By exploiting homology between MuLV proteins, we mapped determinants...
Abstract Background The anti-viral activity of the cellular restriction factor, BST-2/tetherin, was first observed as an ability to block release Vpu-minus HIV-1 from surface infected cells. However, tetherin is also counteracted by primate lentiviruses that do not express a Vpu protein, where anti-tetherin functions are provided either Env protein (HIV-2, SIVtan) or Nef (SIVsm/mac and SIVagm). Within lentiviruses, present in genomes SIVcpz certain SIVsyk viruses. We asked whether, these...
Most studies of HIV latency focus on the peripheral population resting memory T cells, but brain also contains a distinct reservoir HIV-infected cells in microglia, perivascular macrophages, and astrocytes. Studying has been challenging, since live are difficult to recover from autopsy samples primate models SIV infection utilize viruses that more myeloid-tropic than due expression Vpx. Development realistic small animal model would greatly advance this important permit definitive latency....
The cytoplasmic tail of the immature Moloney murine leukemia virus (MoMuLV) envelope protein is approximately 32 amino acids long. During viral maturation, protease cleaves this to release a 16-amino-acid R peptide, thereby rendering fusion competent. A series truncations, deletions, and acid substitutions were constructed in examine its role transduction. Sequential truncation revealed that removal as few 11 resulted significant when was expressed NIH 3T3 cells, similar seen following...
The human immunodeficiency virus type 1 (HIV-1) matrix protein, p17, plays important roles in both the early and late stages of viral life cycle. Using our previously determined solution structure we have undertaken a rational mutagenesis program aimed at mapping structure-function relationships within molecule. Amino acids hypothesized to be for p17 function were mutated examined effect an infectious proviral clone HIV-1. In parallel, analyzed by nuclear magnetic resonance spectroscopy...