Jason Gorman
A5018275034- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Diffusion and Search Dynamics
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
- Microfluidic and Bio-sensing Technologies
- Bacterial Genetics and Biotechnology
- Radiopharmaceutical Chemistry and Applications
- Respiratory viral infections research
- T-cell and B-cell Immunology
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
National Institute of Allergy and Infectious Diseases
2016-2025
National Institutes of Health
2016-2025
Center for Biologics Evaluation and Research
2024-2025
United States Food and Drug Administration
2024
Rensselaer Polytechnic Institute
2024
Hadassah Medical Center
2022
New York Structural Biology Center
2019-2021
Columbia University
2004-2013
Howard Hughes Medical Institute
2010
Intel (United States)
2007-2008
The HIV-1 envelope (Env) mediates viral entry into host cells. To enable the direct imaging of conformational dynamics within Env, we introduced fluorophores variable regions glycoprotein gp120 subunit and measured single-molecule fluorescence resonance energy transfer context native trimers on surface virions. Our observations revealed unliganded Env to be intrinsically dynamic, transitioning between three distinct prefusion conformations, whose relative occupancies were remodeled by...
An antibody to block viral fusion A small fraction of HIV-1–infected individuals develop broad and potent antibodies that bind the HIV-1 envelope protein (Env). These recognize a limited set conserved epitopes on Env, such as Env's host receptor-binding site. Kong et al. now report neutralizing isolated from an individual binds peptide Env. This is unexpected because viruses often try mask key components their cell entry machinery attack. Crystal structures bound Env itself define epitope,...
ABSTRACT The epitopes defined by HIV-1 broadly neutralizing antibodies (bNAbs) are valuable templates for vaccine design, and studies of the immunological development these providing insights vaccination strategies. In addition, most potent reactive bNAbs have potential clinical use. We previously described a family 12 V1V2-directed antibodies, CAP256-VRC26, isolated from an clade C-infected donor at years 1, 2, 4 infection (N. A. Doria-Rose et al., Nature 509:55–62, 2014,...
Most simian-human immunodeficiency viruses (SHIVs) bearing envelope (Env) glycoproteins from primary HIV-1 strains fail to infect rhesus macaques (RMs). We hypothesized that inefficient Env binding CD4 (rhCD4) limits virus entry and replication could be enhanced by substituting naturally occurring simian residues at position 375, which resides a critical location in the CD4-binding pocket is under strong positive evolutionary pressure across broad spectrum of primate lentiviruses. SHIVs...
Current yearly seasonal influenza vaccines primarily induce an antibody response directed against the immunodominant but continually diversifying hemagglutinin (HA) head region. These responses provide protection vaccinating strain little cross-protection other strains or subtypes. To focus immune on subdominant more conserved epitopes HA stem that might protect a broad range of strains, we developed stabilized H1 immunogen lacking displayed ferritin nanoparticle (H1ssF). Here, evaluated B...
The ability of proteins to locate specific targets among a vast excess nonspecific DNA is fundamental theme in biology. Basic principles governing these search mechanisms remain poorly understood, and no study has provided direct visualization single searching for engaging target sites. Here we use the postreplicative mismatch repair MutSα MutLα as model systems understanding diffusion-based searches. Using single-molecule microscopy, directly visualize it searches lesions, lesion-bound...
HIV-1 entry into cells requires binding of the viral envelope glycoprotein (Env) to receptor CD4 and coreceptor. Imaging individual Env molecules on native virions shows trimers be dynamic, spontaneously transitioning between three distinct well-populated conformational states: a pre-triggered (State 1), default intermediate 2) three-CD4-bound conformation 3), which can stabilized by coreceptor-surrogate antibody 17b. Here, using single-molecule Fluorescence Resonance Energy Transfer...