A5100359938- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- HIV Research and Treatment
- Luminescence and Fluorescent Materials
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Vestibular and auditory disorders
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- HIV/AIDS drug development and treatment
- RNA Research and Splicing
- Porphyrin and Phthalocyanine Chemistry
- Molecular Sensors and Ion Detection
- Olfactory and Sensory Function Studies
- Photochromic and Fluorescence Chemistry
- Photodynamic Therapy Research Studies
- RNA modifications and cancer
- Protein Structure and Dynamics
- Glaucoma and retinal disorders
- Microtubule and mitosis dynamics
Central South University
2022-2024
Second Xiangya Hospital of Central South University
2022-2024
Wuhan National Laboratory for Optoelectronics
2022-2024
Chongqing University
2024
State Key Laboratory of Genetic Engineering
2023
Children's Hospital of Fudan University
2023
Hebei Medical University
2023
Second Hospital of Hebei Medical University
2023
Soochow University
2022
The People's Hospital of Guangxi Zhuang Autonomous Region
2022
The HIV-1 envelope (Env) mediates viral entry into host cells. To enable the direct imaging of conformational dynamics within Env, we introduced fluorophores variable regions glycoprotein gp120 subunit and measured single-molecule fluorescence resonance energy transfer context native trimers on surface virions. Our observations revealed unliganded Env to be intrinsically dynamic, transitioning between three distinct prefusion conformations, whose relative occupancies were remodeled by...
Photoexcitation of fluorophores commonly used for biological imaging applications generates reactive oxygen species (ROS) which can cause bleaching the fluorophore and damage to system under investigation. In this study, we show that singlet contributes relatively little Cy5 ATTO 647N photobleaching at low concentrations in aqueous solution. We also significantly less ROS when covalently linked protective agents, cyclooctatetraene (COT), nitrobenzyl alcohol (NBA) or Trolox. Such exhibit...
HIV-1 entry into cells requires binding of the viral envelope glycoprotein (Env) to receptor CD4 and coreceptor. Imaging individual Env molecules on native virions shows trimers be dynamic, spontaneously transitioning between three distinct well-populated conformational states: a pre-triggered (State 1), default intermediate 2) three-CD4-bound conformation 3), which can stabilized by coreceptor-surrogate antibody 17b. Here, using single-molecule Fluorescence Resonance Energy Transfer...
Cyanine fluorophores exhibit greatly improved photostability when covalently linked to stabilizers, such as cyclooctatetraene (COT), nitrobenzyl alcohol (NBA) or Trolox. However, the mechanism by which photostabilization is mediated has yet be determined. Here we present spectroscopic evidence that COT, Cy5, substantially reduces lifetime of Cy5 triplet state, and degree state quenching correlates with enhancements in observed single-molecule fluorescence measurements. By contrast, NBA...
Abstract Dynamic remodelling of intersubunit bridge B2, a conserved RNA domain the bacterial ribosome connecting helices 44 (h44) and 69 (H69) small large subunit, respectively, impacts translation by controlling rotation. Here we show that aminoglycosides chemically related to neomycin—paromomycin, ribostamycin neamine—each bind sites within h44 H69 perturb B2 affect subunit Neomycin paromomycin, which only differ their ring-I 6′-polar group, drive rotation in opposite directions. This...
Bright, long-lasting organic fluorophores enable a broad range of imaging applications. "Self-healing" fluorophores, in which intra-molecularly linked protective agents quench photo-induced reactive species, exhibit both enhanced photostability and biological compatibility. However, the self-healing strategy has yet to achieve its predicted potential, particularly presence ambient oxygen where live-cell studies must often be performed. To identify key bottlenecks this technology that can...
Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness longevity, while simultaneously reducing the generation of reactive oxygen species that promote phototoxicity. Here, we systematically examine self-healing mechanism cyanine-class organic fluorophores spanning visible spectrum. We show Baird aromatic...
ABSTRACT The accumulation and storage of maternal mRNA is crucial for oocyte maturation embryonic development. PATL2 an oocyte-specific RNA-binding protein, previous studies have confirmed that mutation in humans knockout mice cause arrest or development arrest, respectively. However, the physiological function process largely unknown. Here, we report highly expressed growing oocytes couples with EIF4E CPEB1 to regulate expression immature oocytes. germinal vesicle from Patl2−/− exhibit...
Ischemia induces angiogenesis as a compensatory response. Although ischemia is known to causes synthesis and release of calcitonin gene-related peptide (CGRP), it not clear whether CGRP regulates under how does function. Thus we investigated the role in involved mechanisms. We found that level was increased rat hindlimb ischemic tissue. The expression exogenous by adenovirus vectors enhanced blood flow recovery capillary density hindlimbs. In vitro, promoted human umbilical vein endothelial...
The functions of the actin-myosin–based contractile ring in cytokinesis remain to be elucidated. Recent findings show that fission yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization cell wall fibers outside plasma membrane, not ring. Here we one function spatially coordinate septum assembly. We develop an improved method for live-cell imaging division apparatus orienting rod-shaped cells vertically using microfabricated wells. observe hole and are...
Abstract Neurotransmitter:sodium symporters (NSS), targets of antidepressants and psychostimulants, clear neurotransmitters from the synaptic cleft through sodium (Na + )-coupled transport. Substrate Na are thought to be transported extracellular intracellular space an alternating access mechanism by coordinated conformational rearrangements in symporter that alternately expose binding sites each side membrane. However, which ligands coordinates changes occurring on opposite sides membrane...
Metabotropic glutamate receptors (mGluRs) are class C, synaptic G-protein-coupled (GPCRs) that contain large extracellular ligand binding domains (LBDs) and form constitutive dimers. Despite the existence of a detailed picture inter-LBD conformational dynamics structural snapshots both isolated full-length receptors, it remains unclear how mGluR activation proceeds at level transmembrane (TMDs) TMD-targeting allosteric drugs exert their effects. Here, we use time-resolved functional assays...
Abstract Background Osteogenesis is a complex biological process which requires the coordination of multiple molecular mechanisms. This research aimed to explore role and underlying regulatory mechanism circSIPA1L1 during osteogenic differentiation stem cells from apical papilla (SCAPs). Methods EdU retention assay, flow cytometry CCK-8 assay were used evaluate proliferation capacity SCAPs. Western blot alkaline phosphatase (ALP), alizarin red staining (ARS) conducted investigate roles...
Abstract Adaptive optics (AO) is a powerful tool for optical microscopy to counteract the effects of aberrations and improve imaging performance in biological tissues. The diversity sample characteristics entails use different AO schemes measure underlying aberrations. Here, we present an indirect wavefront sensing method leveraging virtual scheme structural-similarity-based shift measurement algorithm enable aberration using intrinsic structures even with temporally varying signals. We...
Mitochondrial calcium uniporter (MCU) is a critical channel for Ca2+ influx into mitochondria. The present study aimed to determine if MCU knockdown has beneficial effects on ischemic brain injury and explore the underlying mechanisms. demonstrated that but not total knockout (KO) attenuated ischemia infarction volume primary cortical neuronal cells' damage. maintained mitochondrial ultrastructure, alleviated overload, reduced apoptosis. Moreover, regulated changes of MICU1 MICU2 after...