A5061230953- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Machine Learning in Bioinformatics
- Virus-based gene therapy research
- Molecular Sensors and Ion Detection
- Plant Virus Research Studies
- RNA Research and Splicing
- RNA regulation and disease
Cornell University
2018-2021
Weill Cornell Medicine
2018-2019
University of Arkansas at Fayetteville
2015-2016
Highlights•Nutrient limitation upregulates the relative expression of rDNA operon, rrnH•Ribosomes bearing 16S rRNA, rrsH, can modulate general stress response•rrsH-bearing ribosomes impact drug resistance, cell motility, and biofilm formation•Naturally occurring rRNA sequence variation encodes altered ribosome functionSummaryPrevailing dogma holds that are uniform in composition function. Here, we show nutrient limitation-induced E. coli changes operons to alter within actively translating...
Metabotropic glutamate receptors (mGluRs) are class C, synaptic G-protein-coupled (GPCRs) that contain large extracellular ligand binding domains (LBDs) and form constitutive dimers. Despite the existence of a detailed picture inter-LBD conformational dynamics structural snapshots both isolated full-length receptors, it remains unclear how mGluR activation proceeds at level transmembrane (TMDs) TMD-targeting allosteric drugs exert their effects. Here, we use time-resolved functional assays...
The metabotropic glutamate receptors (mGluRs) form a family of neuromodulatory G-protein-coupled that contain both seven-helix transmembrane domain (TMD) and large extracellular ligand-binding (LBD) which enables stable dimerization. Although numerous studies have revealed variability across subtypes in the initial activation steps at level LBD dimers, an understanding inter-TMD interaction rearrangement remains limited. Here, we use combination single molecule fluorescence, molecular...
An essential component of mammalian cells, cholesterol exerts significant influence on the physical properties cell membrane and in turn its constituents, including proteins. Although sparse, polar amino acid residues are highly conserved proteins play pivotal roles determining specific structural functional properties. To improve our understanding particular environment, we have examined two "guest" Arg within a well-defined deuterium-labeled "host" framework provided by transmembrane...
Abstract CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single pair resolution. By re-engineering both DNA and protein sequences, we developed collection constitutive inducible vector systems dramatically improve ease efficiency by which nucleotide variants can be created. This new toolkit effective in wide range model systems, provides means for efficient vivo somatic editing.
Metabotropic glutamate receptors (mGluRs) are class C G protein‐coupled (GPCRs) which function in synapses throughout the central nervous system where they respond to excitatory neurotransmitter initiate intracellular signaling cascades. A comprehensive understanding of mGluR activation requires a mechanistic description effects both extracellular ligand binding domain(LBD)‐targeting “orthosteric” and transmembrane domain (TMD)‐targeting “allosteric” compounds, have therapeutic potential....
Abstract The metabotropic glutamate receptors (mGluRs) form a family of neuromodulatory G protein-coupled that contain both seven-helix transmembrane domain (TMD) and large extracellular ligand-binding (LBD) which enables stable dimerization. While numerous studies have revealed variability across subtypes in the initial activation steps at level LBD dimers, an understanding inter-TMD interaction rearrangement remains limited. Here we use combination single molecule fluorescence, molecular...