A5007948012- Protein Structure and Dynamics
- thermodynamics and calorimetric analyses
- Computational Drug Discovery Methods
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Malaria Research and Control
- Glycosylation and Glycoproteins Research
- Spectroscopy and Quantum Chemical Studies
- Immune Cell Function and Interaction
- Photosynthetic Processes and Mechanisms
- DNA and Nucleic Acid Chemistry
- SARS-CoV-2 and COVID-19 Research
- Click Chemistry and Applications
- vaccines and immunoinformatics approaches
- Proteins in Food Systems
- Microbial Metabolic Engineering and Bioproduction
- Pneumocystis jirovecii pneumonia detection and treatment
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- Analytical Chemistry and Chromatography
Johns Hopkins University
2014-2024
Unchained Labs (United States)
2015
Adarza BioSystems (United States)
2013-2014
Johns Hopkins Medicine
1994-2011
Drexel University
2004-2008
Harvard University
2002-2008
Dana-Farber Cancer Institute
2002-2008
Bryn Mawr College
2008
Institute for Molecular Medicine
2008
Universidade de São Paulo
2008
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsothermal titration calorimetryErnesto Freire, Obdulio L. Mayorga, and Martin StraumeCite this: Anal. Chem. 1990, 62, 18, 950A–959APublication Date (Print):September 1, 1990Publication History Published online22 September 2008Published inissue 1 1990https://pubs.acs.org/doi/10.1021/ac00217a002https://doi.org/10.1021/ac00217a002research-articleACS PublicationsRequest reuse permissionsArticle Views11429Altmetric-Citations461LEARN ABOUT THESE...
Achieving sustained drug delivery to mucosal surfaces is a major challenge due the presence of protective mucus layer that serves trap and rapidly remove foreign particulates. Nanoparticles engineered penetrate barriers (mucus-penetrating particles, "MPP") have shown promise for improving distribution, retention efficacy at surfaces. MPP are densely coated with polyethylene glycol (PEG), which shields nanoparticle core from adhesive interactions mucus. However, PEG density required impart...
Abstract The heat capacity plays a major role in the determination of energetics protein folding and molecular recognition. As such, better understanding this thermodynamic parameter its structural origin will provide new insights for development design strategies. In paper we have analyzed absolute proteins different conformations. results these studies indicate that three terms account protein: (1) one term depends only on primary or covalent structure contains contributions from...
Abstract The theoretical basis for the statistical mechanical deconvolution of a thermally induced macromolecular melting profile is presented. It demonstrated that all thermodynamic quantities characterizing multistate transition can be obtained from average excess enthalpy function, 〈Δ H 〉, system, without any assumption particular model or mechanism reaction. Experimentally, 〉 scanning calorimetric data by direct integration apparent molar heat capacity Φ Cp . Once known as continuous...
When interacting with the CD4 receptor, HIV gp120 envelope glycoprotein undergoes conformational changes that allow binding to chemokine receptor. Receptor is proposed lead in gp41 transmembrane involving creation and/or exposure of a coiled coil consisting three heptad repeat (HR) sequences. The subsequent interaction HR2 region this results assembly six-helix bundle promotes fusion viral and target cell membranes. Here we show induces formation HR1 process does not involve shedding depends...
A new statistical thermodynamic formalism has been developed in order to describe the equilibrium folding pathway of proteins. The resulting allows calculation probabilities that individual amino acid residues will be a native or native-like conformation for any given degree protein molecule. residue are defined by probability distribution conformational states and can used calculate experimental quantities like native-state, hydrogen exchange protection factors. combinatorial algorithm...
The discovery that the protease from human immunodeficiency virus (HIV) belongs to aspartic family has generated renewed interest in this class of proteins. In paper, interactions endothiapepsin, an proteinase fungusEndothia parasitica,with inhibitor pepstatin A have been studied by high-sensitivity calorimetric techniques. These experiments permitted a complete characterization temperature and pH-dependence binding energetics. reaction is characterized negative intrinsic enthalpy heat...
Abstract Theoretical estimations of changes in side chain configurational entropy are essential for understanding the different contributions to overall thermodynamic behavior important biological processes like folding and binding. The any given particular protein can be evaluated from complete energy profile chain. Calculations profiles performed using single bond dihedrals as only independent variables long structures at each value allowed relax through small valence angles. probabilities...
The magnitude of the conformational entropy change experienced by peptide backbone upon protein folding was investigated experimentally and computational analysis. Experimentally, two different pairs mutants a 33 amino acid corresponding to leucine zipper region GCN4 were used for high-sensitivity microcalorimetric Each pair differed only having alanine or glycine at specific solvent-exposed position under conditions in which differences stability could be attributed unfolded state. studied...
SARS (severe acute respiratory syndrome) is caused by a newly discovered coronavirus. A key enzyme for the maturation of this virus and, therefore, target drug development main protease 3CLpro (also termed SARS-CoV 3CLpro). We have cloned and expressed in Escherichia coli full-length as well truncated form containing only catalytic domains. The recombinant proteins been characterized enzymatically using fluorescently labeled substrate; their structural stability solution has determined...
Cooperative interactions link the behavior of different amino acid residues within a protein molecule. As result, effects chemical or physical perturbations to any given residue are propagated other by an intricate network interactions. Very often, acids “sense” occurring at very distant locations in In these studies, we have investigated computer simulation structural distribution those We show here that cooperative not intrinsically bi-directional and play roles existing protein. The...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of surface curvature on stability, thermodynamic behavior, and osmotic activity dipalmitoylphosphatidylcholine single lamellar vesiclesD. Lichtenberg, E. Freire, C. F. Schmidt, Y. Barenholz, P. L. Felgner, T. ThompsonCite this: Biochemistry 1981, 20, 12, 3462–3467Publication Date (Print):June 9, 1981Publication History Published online1 May 2002Published inissue 9 June...