A5059728810- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Computational Physics and Python Applications
- Renal Diseases and Glomerulopathies
- SARS-CoV-2 and COVID-19 Research
- Protein Interaction Studies and Fluorescence Analysis
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- COVID-19 Clinical Research Studies
- Chronic Kidney Disease and Diabetes
- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Wnt/β-catenin signaling in development and cancer
- Protein Structure and Dynamics
- Renal and related cancers
- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Medical Image Segmentation Techniques
- Geophysical and Geoelectrical Methods
- Computer Graphics and Visualization Techniques
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Streptococcal Infections and Treatments
Goethe University Frankfurt
2020-2025
Chan Zuckerberg Initiative (United States)
2024
Universität Hamburg
2024
University Medical Center Hamburg-Eppendorf
2024
Czech Academy of Sciences, Institute of Biophysics
2024
Max Planck Institute of Biophysics
2020
Yale University
2018-2019
Abstract Aims Coronavirus disease 2019 is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and has emerged as a global pandemic. SARS-CoV-2 infection can lead to elevated markers of cardiac injury associated with higher risk mortality. It unclear whether direct cardiomyocytes or mainly secondary lung inflammation. Here, we investigate are permissive for infection. Methods results Two strains infected human induced pluripotent stem cell-derived demonstrated detection...
Abstract Cryo‐electron tomography analysis involves the selection of macromolecular complexes to be used for subsequent sub‐tomogram averaging and structure determination. Here, we describe a plugin developed UCSF ChimeraX that allows display, selection, editing particles within tomograms. Positions orientations selected can manually set, modified inspected in real time, both on screen virtual reality, exported various file formats. The parallel visualization stored several meta data lists,...
Desmosomes are cell-cell junctions that link tissue cells experiencing intense mechanical stress. Although the structure of desmosomal cadherins is known, desmosome architecture-which essential for mediating numerous functions-remains elusive. Here, we recorded cryo-electron tomograms (cryo-ET) in which individual can be discerned; they appear variable shape, spacing, and tilt with respect to membrane. The resulting sub-tomogram average reaches a resolution ∼26 Å, limited by inherent...
Abstract A major challenge in electron cryo‐microscopy (ECM) imaging is preparing the protein specimen without artifacts caused by surface tension at air‐water interface (AWI). Here, we report nanosecond hyperquenching (NHQ) – a method of ECM samples AWI‐bound macromolecules. The fast narrow sample jet impinges eutectic propane‐ethane (PET) liquid cryogen 77 K and breaks up, forming 30–150‐nm‐thick vitrified films. NHQ films with particles are formed directly PET cryogen, precluding AWI...
A high throughput processing pipeline that performs comprehensive corrections is needed to realize the full potential of cryo-electron tomography and subtomogram averaging. The field's fragmented software landscape remains a significant hurdle this end. Here we present AreTomoLive, an automated real-time composed two GPU-accelerated packages. first, AreTomo3, streamlines tomographic alignment reconstruction, with new features fully account for sample geometry, locally correct contrast...
<title>Abstract</title> A high throughput processing pipeline that performs comprehensive corrections is needed to realize the full potential of cryo-electron tomography and subtomogram averaging. The field’s fragmented software landscape remains a significant hurdle this end. Here we present AreTomoLive, an automated real-time composed two GPU-accelerated packages. first, AreTomo3, streamlines tomographic alignment reconstruction, with new features fully account for sample geometry, locally...
Background: The coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome 2 (SARS-CoV-2) and has emerged as global pandemic. SARS-CoV-2 infection can lead to elevated markers of cardiac injury associated with higher risk mortality in COVID-19 patients. It unclear whether may have been direct cardiomyocytes or mainly secondary lung inflammation. Here we investigate human are permissive for infection. Methods: Infection was induced two strains (FFM1 FFM2) pluripotent...
Abstract Vertebrate life depends on renal function to filter excess fluid and remove low-molecular-weight waste products. An essential component of the kidney filtration barrier is slit diaphragm (SD), a specialized cell-cell junction between podocytes. Although constituents SD are largely known, its molecular organization remains elusive. Here, we use super-resolution correlative light electron microscopy quantify linear rate reduction in albumin concentration across barrier. Next,...
The nap particle is an immunogenic surface adhesion complex from Mycoplasma genitalium . It essential for motility and responsible binding sialylated oligosaccharides on the of host cell. composed two P140-P110 heterodimers, structure which was recently solved. However, interpretation mechanism by mycoplasma cells orchestrate remained challenging. Here, we provide cryo-electron tomography structures at ~11 Å resolution, allow distinction between bound released state particle, displaying in...
Abstract Cryo-electron tomography analysis involves the selection of macromolecular complexes to be used for subsequent sub-tomogram averaging and structure determination. Here, we describe a plugin developed UCSF ChimeraX that allows display, selection, editing particles within tomograms. Positions orientations selected can manually set, modified inspected in real-time, both on screen virtual reality, exported various file formats. The parallel visualization stored several meta data lists,...
Abstract Cryo-electron tomography (cryoET) has emerged as a powerful structural biology tool for understanding protein complexes in their native cellular environments. Presently, 3D volumes of environments can be acquired the thousands few days where each volume provides rich and complex landscape. Despite numerous innovations, localizing identifying vast majority species these remains prohibitively difficult. Machine learning based methods provide an opportunity to automate process labeling...
Abstract Vertebrate life depends on renal function to filter excess fluid and remove low molecular-weight waste products. An essential component of the kidney filtration barrier is slit diaphragm (SD), a specialized cell-cell junction between podocytes. Although constituents SD have recently been identified, its molecular architecture remains elusive. Here, we use cryo-electron tomography vitreous lamellae from high-pressure frozen native murine glomeruli analyse SD. In our tomographic...
The functioning of vertebrate life relies on renal filtration surplus fluid and elimination low-molecular-weight waste products, while keeping serum proteins in the blood. In disease, however, there is leak tracing them to identify leaking position within tissue with a nanometer resolution poses significant challenge. Correlative microscopy integrates specificity fluorescent protein labeling into high-resolution electron micrographs. Using chemical tagging albumin synthetic fluorophores we...
The enhancement of biomolecular image analysis and data interpretation is significantly improved through the application advanced visualization techniques. Numerous packages are currently available, spanning a broad spectrum applications. Recently, we have extended capabilities UCSF ChimeraX to address specific demands cryo-electron tomography. Here, introduce evolution our existing plugin, ArtiaX, designed generate models that facilitate particle selection, define camera recording paths,...
Abstract We are launching a machine learning (ML) competition focused on particle picking in cryo-electron tomography (cryoET) data, crucial task structural biology. To support this, we have created comprehensive suite of open-source tools to develop resources for our competition, including copick dataset management, napari plugins interactive visualization, utilities converting picks segmentation masks, and PyTorch custom sampling. These streamline the processes data handling, labeling,...
Abstract The direct study of transcription or DNA–protein-binding events, requires imaging individual genes at molecular resolution. Electron microscopy (EM) can show local detail the genome. However, visualization and analysis specific is currently not feasible as they cannot be unambiguously localized in crowded, landmark-free environment nucleus. Here, we present a method for genomic insertion gene clusters that imaged together with their associated protein complexes EM. uses CRISPR/Cas9...