A5004648123- Renal Diseases and Glomerulopathies
- Ion Transport and Channel Regulation
- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Chronic Kidney Disease and Diabetes
- Hormonal Regulation and Hypertension
- Ion channel regulation and function
- Electrolyte and hormonal disorders
- Pancreatic function and diabetes
- Renal Transplantation Outcomes and Treatments
- Cardiac electrophysiology and arrhythmias
- Biomedical Research and Pathophysiology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Renal cell carcinoma treatment
- Wnt/β-catenin signaling in development and cancer
- Adenosine and Purinergic Signaling
- Pluripotent Stem Cells Research
- Lysosomal Storage Disorders Research
- Diabetes and associated disorders
- Advanced Electron Microscopy Techniques and Applications
- Reproductive Biology and Fertility
- Organ Donation and Transplantation
- Amyloidosis: Diagnosis, Treatment, Outcomes
- COVID-19 Clinical Research Studies
- Organ Transplantation Techniques and Outcomes
University Medical Center Hamburg-Eppendorf
2017-2025
Universität Hamburg
2017-2025
Goethe University Frankfurt
2024
University of Freiburg
2002-2019
University Medical Center Freiburg
2010-2018
RELX Group (United States)
2018
Metabolism and Renal Physiology
2014
University of Tübingen
2004-2007
Innsbruck Medical University
2005
Institut de Pharmacologie Moléculaire et Cellulaire
2001
Injury and loss of podocytes are leading factors glomerular disease renal failure. The postmitotic podocyte is the primary target for toxic, immune, metabolic, oxidant stress, but little known about how this cell type copes with stress. Recently, autophagy has been identified as a major pathway that delivers damaged proteins organelles to lysosomes in order maintain cellular homeostasis. Here we report exhibit an unusually high level constitutive autophagy. Podocyte-specific deletion...
We detected a protein in rabbit skeletal muscle extracts that was phosphorylated rapidly by SGK1 (serum- and glucocorticoid-induced kinase 1), but not Bα, identified it as NDRG2 (N-myc downstream-regulated gene 2). at Thr330, Ser332 Thr348in vitro. All three residues were from wild-type mice, mice do express SGK1. also the related NDRG1 isoform Thr328, Ser330 Thr346 (equivalent to Thr348 of NDRG2), well Thr356 Thr366. Residues Thr346, Thr366 are located within identical decapeptide sequences...
Mutations in the gene encoding for K + channel α-subunit KCNQ1 have been associated with long QT syndrome and deafness. Besides heart inner ear epithelial cells, is expressed a variety of cells including renal proximal tubule gastrointestinal tract cells. At these sites, cellular ions exit through complexes, which may serve to recycle or maintain cell membrane potential thus driving force electrogenic transepithelial transport, e.g., Na /glucose cotransport. Employing pharmacologic...
Podocyte loss is a major determinant of progressive CKD. Although recent studies showed that subset parietal epithelial cells can serve as podocyte progenitors, the role turnover and regeneration in repair, aging, nephron remains unclear. Here, we combined genetic fate mapping with highly efficient isolation protocols to precisely quantify regeneration. We demonstrate give rise fully differentiated visceral indistinguishable from resident podocytes limited renewal occurs diphtheria toxin...
Kidney podocytes are highly differentiated epithelial cells that form interdigitating foot processes with bridging slit diaphragms (SDs) regulate renal ultrafiltration. Podocyte injury results in proteinuric kidney disease, and genetic deletion of SD-associated CD2-associated protein (CD2AP) leads to progressive failure mice humans. Here, we have shown CD2AP regulates the TGF-β1-dependent translocation dendrin from SD nucleus. Nuclear acted as a transcription factor promote expression...
Damage to and loss of glomerular podocytes has been identified as the culprit lesion in progressive kidney diseases. Here, we combine mass spectrometry-based proteomics with mRNA sequencing, bioinformatics, hypothesis-driven studies provide a comprehensive quantitative map mammalian that identifies unanticipated signaling pathways. Comparison vivo datasets data from podocyte cell cultures showed limited value available culture models. Moreover, stable isotope labeling by amino acids...
Article19 June 2018Open Access Source DataTransparent process Cilia-localized LKB1 regulates chemokine signaling, macrophage recruitment, and tissue homeostasis in the kidney Amandine Viau Renal Department, University Medical Center, Freiburg, Germany Faculty of Medicine, INSERM U1151, Institut Necker Enfants Malades, Department Growth Signaling, Université Paris Descartes-Sorbonne Cité, Paris, France Search for more papers by this author Frank Bienaimé Service d'Explorations Fonctionnelles,...
Significance Mammalian target of rapamycin complex 1 (mTORC1) inhibitors are commonly used as immunosuppressants in solid-organ transplantation and antiproliferative agents various cancers. Despite indications serious renal adverse events caused by mTORC1 inhibition, the role for epithelial function homeostasis has remained elusive. Unexpectedly, tubular controls energy-driven urine-concentrating mechanisms maintaining mitochondrial biogenesis. Under pathophysiological conditions,...
Renal proximal tubular cells constantly recycle nutrients to ensure minimal loss of vital substrates into the urine. Although most transport mechanisms have been discovered at molecular level, little is known about factors regulating these processes. Here, we show that mTORC1 and mTORC2 specifically synergistically regulate PTC endocytosis Using a conditional mouse genetic approach disable nonredundant subunits mTORC1, mTORC2, or both, showed mice lacking mTORC1/mTORC2 but not alone develop...
ATPase H+-transporting lysosomal accessory protein 2 (Atp6ap2), also known as the (pro)renin receptor, is a type 1 transmembrane and an subunit of vacuolar H+-ATPase (V-ATPase) that may function within renin-angiotensin system. However, contribution Atp6ap2 to renin-angiotensin-dependent functions remains unconfirmed. Using mice with inducible conditional deletion in mouse renal epithelial cells, we found decreased V-ATPase expression activity intercalated cells collecting duct impaired...
Podocyte injury is an early event in diabetic kidney disease and a hallmark of glomerulopathy. MicroRNA-146a (miR-146a) highly expressed many cell types under homeostatic conditions, plays important anti-inflammatory role myeloid cells. However, its podocytes unclear. Here, we show that miR-146a expression levels decrease the glomeruli patients with type 2 diabetes (T2D), which correlates increased albuminuria glomerular damage. are also significantly reduced albuminuric BTBR ob/ob mice,...
Current therapies for Fabry disease are based on reversing intracellular accumulation of globotriaosylceramide (Gb3) by enzyme replacement therapy (ERT) or chaperone-mediated stabilization the defective enzyme, thereby alleviating lysosomal dysfunction. However, their effect in reversal end-organ damage, like kidney injury and chronic disease, remains unclear. In this study, ultrastructural analysis serial human biopsies showed that long-term use ERT reduced Gb3 podocytes but did not reverse...
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and histopathologic glomerular lesions are among earliest structural alterations DN. However, signaling pathways that initiate these incompletely understood.To delineate cellular molecular basis for DN initiation, we performed single-cell bulk RNA sequencing cells from type 2 diabetes mice (BTBR ob/ob) at early stage DN.Analysis differentially expressed genes revealed glucose-independent responses in cell types. The...
The electrochemical gradient for K+ across the luminal membrane of proximal tubule favors fluxes to lumen. Here it was demonstrated by immunohistochemistry that KCNE1 and KCNQ1, which form together slowly activated component delayed rectifying current in heart, also colocalize mouse kidney. Micropuncture experiments revealed a reduced concentration late early distal tubular fluid as well delivery these sites knockout (-/-), compared with wild-type (+/+) mice. These observations would be...
Vertebrate life critically depends on renal filtration and excretion of low molecular weight waste products. This process is controlled by a specialized cell-cell contact between podocyte foot processes: the slit diaphragm (SD). Using comprehensive set targeted KO mice key SD molecules, we provided genetic, functional, high-resolution ultrastructural data highlighting concept flexible, dynamic, multilayered architecture SD. Our indicate that mammalian composed NEPHRIN NEPH1 while NEPH2 NEPH3...
Mechanosensitive activation of YAP is an early response in damaged kidneys.
Background Nephron number is a major determinant of long-term renal function and cardiovascular risk. Observational studies suggest that maternal nutritional metabolic factors during gestation contribute to the high variability nephron endowment. However, underlying molecular mechanisms have been unclear. Methods We used mouse models, including DNA methyltransferase ( Dnmt1, Dnmt3a, Dnmt3b ) knockout mice, optical projection tomography, three-dimensional reconstructions nephrogenic niche,...
Introduction: C-reactive protein circulates as a pentameric (pCRP). pCRP is well-established diagnostic marker plasma levels rise in response to tissue injury and inflammation. We recently described pro-inflammatory properties of CRP, which are mediated by conformational changes from bioactive isoforms expressing neo-epitopes (pCRP* mCRP). Here, we investigate the role CRP renal ischemia/reperfusion (IRI). Methods: Rat kidneys animals with without intraperitoneally injected were subjected...