A5024276744- Renal Diseases and Glomerulopathies
- Education and Learning Interventions
- Chronic Kidney Disease and Diabetes
- Education, Safety, and Science Studies
- Nanowire Synthesis and Applications
- Platelet Disorders and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Bacterial biofilms and quorum sensing
- Neonatal Respiratory Health Research
- Cell Adhesion Molecules Research
- Cystic Fibrosis Research Advances
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- CAR-T cell therapy research
- Cellular transport and secretion
- Educational Research and Pedagogy
- Educational Systems and Policies
- Cell death mechanisms and regulation
- Lymphatic System and Diseases
- Nanofabrication and Lithography Techniques
- Chronic Lymphocytic Leukemia Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Degradation and Inhibitors
- Psychosocial Factors Impacting Youth
- Biological Research and Disease Studies
St. Jude Children's Research Hospital
2023-2024
Rush University Medical Center
2015-2021
Czech Academy of Sciences, Institute of Physiology
2016
Johns Hopkins Medicine
2013
Johns Hopkins University
2013
Columbia University
2013
Duke Medical Center
2012
Genetic variations in the ITGAM gene (encoding CD11b) strongly associate with risk for systemic lupus erythematosus (SLE). Here we have shown that 3 nonsynonymous variants produce defective CD11b elevated levels of type I interferon (IFN-I) lupus, suggesting a direct link between reduced activity and chronically increased inflammatory status patients. Treatment small-molecule agonist LA1 led to partial integrin activation, IFN-I responses WT but not CD11b-deficient mice, protected...
Podocyte injury is an early event in diabetic kidney disease and a hallmark of glomerulopathy. MicroRNA-146a (miR-146a) highly expressed many cell types under homeostatic conditions, plays important anti-inflammatory role myeloid cells. However, its podocytes unclear. Here, we show that miR-146a expression levels decrease the glomeruli patients with type 2 diabetes (T2D), which correlates increased albuminuria glomerular damage. are also significantly reduced albuminuric BTBR ob/ob mice,...
A lack of relevant genetic models and cell lines hampers our understanding hepatoblastoma pathogenesis the development new therapies for this neoplasm. Here, we report an improved MYC-driven hepatoblastoma-like murine model that recapitulates pathological features embryonal type hepatoblastoma, with transcriptomics resembling high-risk gene signatures human disease. Single-cell RNA-sequencing spatial identify distinct subpopulations cells. After deriving from mouse model, map cancer...
Podocyte injury and loss mark an early step in the pathogenesis of various glomerular diseases, making these cells excellent targets for therapeutics. However, cell-based high-throughput screening assays rational development podocyte-directed therapeutics are currently lacking. Here, we describe a novel high-content screening-based phenotypic assay that analyzes thousands podocytes per condition 96-well plates to quantitatively measure dose-dependent changes multiple cellular features. Our...
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that causes serious infections in immunocompromised individuals and cystic fibrosis patients. ExoS ExoT are two homologous bifunctional Type III Secretion System (T3SS) virulence factors induce apoptosis target host cells. They possess GTPase Activating Protein (GAP) domain at their N-termini, which share ~76% homology, an ADP-ribosyltransferase (ADPRT) C-termini, non-overlapping substrates. Both the GAP ADPRT domains...
Chronic lung infection in cystic fibrosis leads to an inflammatory response that persists because of the chronic presence bacteria and ultimately a catastrophic failure function.We use combination biochemistry, cell molecular biology study interaction TRADD, key adaptor molecule TNFα signaling, with CFTR regulation NFκB.We show Wt binds colocalizes TRADD. TRADD is signaling intermediate connecting activation NFκB. By contrast, ΔF508 does not bind NF-κB higher CFBE expressing than cells CFTR....
Magnetic resonance histology (MRH) has become a valuable tool in evaluating drug-induced toxicity preclinical models. However, its application renal injury been limited. This study tested the hypothesis that MRH could detect image-based biomarkers of chronic disease, inflammation, or age-related degeneration kidney, laying foundation for more extensive use drug toxicity. We examined entire intact kidney spontaneous model progressive nephropathy. Kidneys from male Sprague Dawley rats were...
Rho family GTPases, the prototypical members of which are Cdc42, Rac1, and RhoA, molecular switches best known for regulating actin cytoskeleton. In addition to canonical small large GTPase dynamin has been implicated in cytoskeleton via direct dynamin-actin interactions. The physiologic role linked maintenance kidney filtration barrier. Additionally, molecule Bis-T-23, promotes actin–dependent oligomerization thus, increases polymerization, improved renal health diverse models CKD,...
CAR T cell therapy is a promising approach to improve outcomes and decrease toxicities for patients with cancer. While extraordinary success has been achieved using cells treat CD19-positive malignancies, multiple obstacles have so far limited the benefit of solid tumors. Novel manufacturing engineering approaches show great promise enhance function against However, similar single agent chemotherapy approaches, monotherapy may be unable achieve high cure rates difficult Thus, combinatorial...
Abstract PROTAC® (proteolysis-targeting chimera) molecules induce proximity between an E3 ligase and protein-of-interest (POI) to target the POI for ubiquitin-mediated degradation. Cooperative E3-PROTAC-POI complexes have potential achieve neo-substrate selectivity beyond that established by binding ligand alone. Here, we extend collection of ubiquitin ligases employable cooperative ternary complex formation include C-degron KLHDC2. Ligands were identified engage site in KLHDC2, subjected...
Soluble urokinase receptor (suPAR) is a circulatory molecule that activates αvβ3 integrin on podocytes, causes foot process effacement, and contributes to proteinuric kidney disease. While active can be targeted by antibodies small molecules, endogenous inhibitors haven't been discovered yet. Here we report what believe novel renoprotective role for the inducible costimulator ligand (ICOSL) in early disease through its selective binding podocyte integrin. Contrary ICOSL's immune-regulatory...
Podocyte dysfunction and loss is an early event a hallmark of proteinuric kidney diseases. A podocyte's normal function maintained via its unique cellular architecture that relies on intracellular network filaments, including filamentous actin (F-actin) microtubules, provides mechanical support. Damage to this leads changes in morphology results podocyte injury, dysfunction, death. Conversely, stabilization protects podocytes ameliorates proteinuria. This suggests could be key therapeutic...
Low levels of insulin-like growth factor 1 (IGF-1) have been observed in the serum cystic fibrosis (CF) patients. However, effects low IGF-1 on transmembrane conductance regulator (CFTR), whose defective function is primary cause fibrosis, not studied. Here, we show human cells that increases steady-state mature wildtype CFTR a CFTR-associated ligand (CAL)- and TC10-dependent manner; moreover, CFTR-mediated chloride transport. Using an acceptor photobleaching fluorescence resonance energy...
ABSTRACT CAR T cell therapy is a promising approach to improve outcomes and decrease toxicities for patients with cancer. While extraordinary success has been achieved using cells treat CD19-positive malignancies, multiple obstacles have so far limited the benefit of solid tumors. Novel manufacturing engineering approaches show great promise enhance function against However, similar single agent chemotherapy approaches, monotherapy may be unable achieve high cure rates difficult Thus,...
Objectives The purpose of this study is to analyze the lecture preparation, characteristics, and satisfaction adult continuing education learners in childcare sites process converting person online lectures. Methods To end, author examined syllabus, LMS SNS materials, PPT, learners' self-report data. ‘The guidance child life’ as a synchronous video conferencing ‘Advanced Child Counseling’ recording conducted second semester 2021 with Merriam qualitative analysis. participant was total 65...
Objectives The purpose of this study is to present a theoretical framework by examining and analyzing the natural experience effects attention deficit hyperactivity in children with at-risk ADHD. This based on perceptions early childhood teachers using grounded theory. Methods To end, in-depth interviews were conducted total five directors education institutions who had conducting nature experiences such as outdoor play, plant growing, ecological including forest experience, collected data...
CYP3A5 is a cytochrome P450 (CYP) enzyme that metabolizes drugs and contributes to drug resistance in cancer. However, it remains unclear whether directly influences cancer progression. In this report, we demonstrate regulates glucose metabolism pancreatic ductal adenocarcinoma. Multi-omics analysis showed knockdown results decrease various glucose-related metabolites through its effect on transport. A mechanistic study revealed enriches the transporter GLUT1 at plasma membrane by...
본 연구는 예비 유아교사의 생애핵심능력과 회복탄력성의 경향성을 살펴보고 이들 간의 관련성과 영향력을 알아보고자 하였다. 연구대상은 경기도 소재 4년제 대학의 유아교육과 학생 189명이었으며 대상 평균연령은 만 19.82세(SD= 2.09)이었다. 53개 문항으로 구성된 한국형 회복탄력성 지수 검사(KRQ-53)와 134개 생애능력 측정 도구를 사용하여 2019년 10월 7일부터 28일까지 자가보고식 설문을 시행하였다. 수집된 자료는 SPSS 22.0 프로그램을 활용하여 Pearson 상관분석과 중다회귀분석을 실시하였다. 연구의 결과 예비유아교사의 회복탄력성은 전체적으로 '보통이상' 수준이며 생애핵심능력은 '보통' 수준으로 나타났다. 세부 항목으로는 대인관계능력이 가장 높게 나타났으며, 그 중에서도 자아확장력이 상관분석결과 회복탄력성과 생애핵심능력의 하위요인들 간에는 유의한 정적 상관관계가 있었다. 중 자기 조절능력의 원인분석력과 대인관계의 공감능력은 모든 하위요소와 높은...