A5004496871- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Connexins and lens biology
- Single-cell and spatial transcriptomics
- Conducting polymers and applications
- Yersinia bacterium, plague, ectoparasites research
- Electrospun Nanofibers in Biomedical Applications
- Urological Disorders and Treatments
- Photoacoustic and Ultrasonic Imaging
- Parathyroid Disorders and Treatments
- Genetic and Kidney Cyst Diseases
- Pancreatic function and diabetes
- Urologic and reproductive health conditions
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Ion Transport and Channel Regulation
- Genetic Syndromes and Imprinting
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Silk-based biomaterials and applications
- Hearing, Cochlea, Tinnitus, Genetics
- Renal cell carcinoma treatment
- Genital Health and Disease
- Neuroscience of respiration and sleep
- Dermatologic Treatments and Research
Bursa Uludağ Üni̇versi̇tesi̇
2025
University Medical Center Hamburg-Eppendorf
2020-2023
Universität Hamburg
2020-2023
Izmir Institute of Technology
2016-2019
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and histopathologic glomerular lesions are among earliest structural alterations DN. However, signaling pathways that initiate these incompletely understood.To delineate cellular molecular basis for DN initiation, we performed single-cell bulk RNA sequencing cells from type 2 diabetes mice (BTBR ob/ob) at early stage DN.Analysis differentially expressed genes revealed glucose-independent responses in cell types. The...
Expansion microscopy physically enlarges biological specimens to achieve nanoscale resolution using diffraction-limited systems1. However, optimal performance is usually reached laser-based systems (for example, confocal microscopy), restricting its broad applicability in clinical pathology, as most centres have access only light-emitting diode (LED)-based widefield systems. As a possible alternative, computational method for image enhancement, namely, super-resolution radial fluctuations...
Abstract The filtration of blood in the kidney which is crucial for mammalian life determined by slit-diaphragm, a cell-cell junction between foot processes renal podocytes. slit-diaphragm thought to operate as final barrier or molecular sensor filtration. Using high-resolution proteomic analysis slit-diaphragms affinity-isolated from rodent kidney, we show that native built junction-forming components Nephrin, Neph1 and Podocin co-assembled high-molecular weight network proteins....
The glomerular filtration barrier (GFB) produces primary urine and is composed of a fenestrated endothelium, basement membrane (GBM), podocytes, slit diaphragm. Impairment the GFB leads to albuminuria microhematuria. GBM generated via secreted proteins from both endothelial cells podocytes supposed majorly contribute selectivity. While genetic mutations or variations components have been recently proposed be common cause diseases, pathways modifying stabilizing remain incompletely...
Sodium retention is the hallmark of nephrotic syndrome (NS) and mediated by proteolytic activation epithelial sodium channel (ENaC) aberrantly filtered serine proteases. Plasmin highly abundant in urine has been proposed to be principal protease responsible for ENaC NS. However, a proof essential role plasmin experimental NS lacking.We used genetic mouse model based on an inducible podocin knockout (Bl6-Nphs2tm3.1Antc *Tg(Nphs1-rtTA*3G)8Jhm *Tg(tetO-cre)1Jaw or nphs2Δipod ). These mice were...
Background: Gap junctions facilitate exchange of small molecules between adjacent cells, serving a crucial function for the maintenance cellular homeostasis.Mutations in connexins, basic unit gap junctions, are associated with several human hereditary disorders.For example, mutations connexin26 (Cx26) cause both non-syndromic deafness and syndromic skin abnormalities such as keratitisichthyosis-deafness (KID) syndrome.These can alter formation junction channels through different mechanisms,...
Polypyrrole (PPy) is an attractive scaffold material for tissue engineering with its non-toxic and electrically conductive properties. There has not been enough information about PPy usage in skin engineering. The aim of this study to investigate biocompatibility polyacrilonitrile (PAN)/PPy nanofibrous human keratinocytes. PAN/PPy bicomponent nanofibers were prepared by electrospinning, various concentrations carbon nanotube (CNT) incorporation. average diameter electrospun decreased...
Abstract Introduction Connexins (Cx) play essential roles in cellular homeostasis by forming gap junctions and non-junctional hemichannels. In vitro characterization of Cx26 mutations causing keratitis-ichthyosis-deafness (KID) syndrome, were shown to form leaky The molecular/cellular mechanisms affected aberrant hemichannels have recently been elucidated. Here, we further wanted characterize KID syndrome mutations, D50A A88V, which remained unaddressed the literature. Methods Neurobiotin...
Abstract Diabetic nephropathy (DN) is the leading cause of end-stage renal disease and histopathologic glomerular lesions are among earliest structural alterations DN. However, signaling pathways that initiate these incompletely understood. To delineate cellular molecular basis for DN initiation, we performed single-cell bulk RNA sequencing cells from type 2 diabetes mice (BTBR ob/ob ) at early stage Analysis differentially expressed genes revealed glucose-independent responses in cell...