Milagros N. Wong
A5102745376- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- COVID-19 Clinical Research Studies
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Lysosomal Storage Disorders Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Diabetes and associated disorders
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Genetic and Kidney Cyst Diseases
- Immune responses and vaccinations
- Liver Disease Diagnosis and Treatment
- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- 3D Printing in Biomedical Research
- Biomedical Research and Pathophysiology
- Renal and related cancers
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- COVID-19 and healthcare impacts
- Atherosclerosis and Cardiovascular Diseases
University Medical Center Hamburg-Eppendorf
2019-2024
Universität Hamburg
2019-2024
Aarhus University
2023-2024
Aarhus University Hospital
2023-2024
RELX Group (United States)
2023
Discovery Institute
2016
Tissue-resident memory-like T H 17 cells are clonally expanded in bronchoalveolar lavage fluid of patients with severe COVID-19.
Extrapulmonary manifestations of COVID-19 have gained attention due to their links clinical outcomes and potential long-term sequelae1. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) displays tropism towards several organs, including the heart kidney. Whether it also directly affects liver has been debated2,3. Here we provide clinical, histopathological, molecular bioinformatic evidence for hepatic SARS-CoV-2. We find that injury, indicated by a high frequency abnormal function...
Macrophages are functionally heterogeneous cells essential for apoptotic cell clearance. Apoptotic defined by homogeneous characteristics, ignoring their original lineage identity. We found that in an interleukin-4 (IL-4)-enriched environment, the sensing of neutrophils macrophages triggered tissue remodeling signature. Engulfment hepatocytes promoted a tolerogenic phenotype, whereas phagocytosis T had little effect on IL-4-induced gene expression. In mouse model parasite-induced pathology,...
Although it is well established that microbial infections predispose to autoimmune diseases, the underlying mechanisms remain poorly understood. After infection, tissue-resident memory T (TRM) cells persist in peripheral organs and provide immune protection against reinfection. However, whether TRM participate responses unrelated primary such as inflammation, unknown. By using high-dimensional single-cell analysis, we identified CD4+ with a TH17 signature (termed TRM17 cells) kidneys of...
The cellular origins of glomerulosclerosis involve activation parietal epithelial cells (PECs) and progressive podocyte depletion. While mammalian target rapamycin-mediated (mTOR-mediated) hypertrophy is recognized as an important signaling pathway in the context glomerular disease, role a compensatory mechanism preventing PEC remains poorly understood. In this study, we show that mTOR activation-related genes were both upregulated intercorrelated biopsies from patients with focal segmental...
Current therapies for Fabry disease are based on reversing intracellular accumulation of globotriaosylceramide (Gb3) by enzyme replacement therapy (ERT) or chaperone-mediated stabilization the defective enzyme, thereby alleviating lysosomal dysfunction. However, their effect in reversal end-organ damage, like kidney injury and chronic disease, remains unclear. In this study, ultrastructural analysis serial human biopsies showed that long-term use ERT reduced Gb3 podocytes but did not reverse...
Expansion microscopy physically enlarges biological specimens to achieve nanoscale resolution using diffraction-limited systems1. However, optimal performance is usually reached laser-based systems (for example, confocal microscopy), restricting its broad applicability in clinical pathology, as most centres have access only light-emitting diode (LED)-based widefield systems. As a possible alternative, computational method for image enhancement, namely, super-resolution radial fluctuations...
Podocyte depletion is sufficient for the development of numerous glomerular diseases and can be absolute (loss podocytes) or relative (reduced number podocytes per volume glomerulus). Commonly used methods to quantify podocyte introduce bias, whereas gold standard stereologic methodologies are time consuming impractical. We developed a novel approach assessing in whole glomeruli that combines immunofluorescence, optical clearing, confocal microscopy, three-dimensional analysis. validated...
Morphologic examination of tissue biopsies is essential for histopathological diagnosis. However, accurate and scalable cellular quantification in human samples remains challenging. Here, we present a deep learning–based approach antigen-specific morphometrics kidney biopsies, which combines indirect immunofluorescence imaging with U-Net–based architectures image-to-image translation dual segmentation tasks, achieving human-level accuracy. In the kidney, podocyte loss represents hallmark...
Staphylococcus aureus is frequently detected in patients with sepsis and thus represents a major health burden worldwide. CD4+ T helper cells are involved the immune response to S. by supporting antibody production phagocytosis. In particular, Th1 Th17 secreting IFN-γ IL-17A, control of systemic infections humans mice. To investigate role severe infections, we established mouse model which kidney was identified be organ highest bacterial load abundance cells. this model, IL-17A but not...
A role of CD4+ T cells during the progression from nonalcoholic fatty liver disease (NAFLD) to steatohepatitis (NASH) has been suggested, but which polarization state these characterizes this and development fibrosis remain unclear. In addition, a gut-liver axis suggested play in NASH, just begun be investigated. Combining single-cell RNA sequencing multiple-parameter flow cytometry, we provide first cell atlas our knowledge focused on liver-infiltrating patients with NAFLD showing that NASH...
Super-resolution microscopy (SRM) has revolutionized life sciences by overcoming the diffraction limit, enabling visualization of biological structures at nanoscale. Expansion Microscopy (ExM) emerged as a powerful and accessible technique that enhances resolution physically enlarging specimen. Importantly, principles ExM provide unique foundation for combinations with SRM methods, pushing boundaries achievable resolution. This review explores fundamental examines its successful integration...
Transcript quantification tools efficiently map bulk RNA sequencing (RNA-seq) reads to reference transcriptomes. However, their output consists of transcript count estimates that are subject multiple biases and cannot be readily used with existing differential gene expression analysis in Python.Here we present pytximport, a Python implementation the tximport R package supports variety input formats, different modes bias correction, inferential replicates, gene-level summarization counts,...
Abstract Morphologic examination of tissue biopsies is essential for histopathological diagnosis. However, accurate and scalable cellular quantification in human samples remains challenging. Here, we present a deep learning-based approach antigen-specific morphometrics kidney biopsies, which combines indirect immunofluorescence imaging with U-Net-based architectures image-to-image translation dual segmentation tasks, achieving human-level accuracy. In the kidney, podocyte loss represents...
Abstract DNA repair is essential for preserving genome integrity and ensuring cellular functionality survival. Podocytes, post-mitotic glomerular epithelial cells, bear limited regenerative capacity, their survival indispensable to maintain the function of kidney’s filtration units. While podocyte depletion a hallmark aging process many proteinuric kidney diseases, underlying factors remain unclear. We investigated in diseases by using constitutive an inducible podocyte-specific knockout...
Abstract Stroke and central nervous system dysfunction are cardinal symptoms in critically ill corona virus disease 19 (COVID-19) patients. In an autopsy series of 32 COVID-19 patients, we investigated whether carotid arteries were infected with SARS-CoV-2 by employing genomic, virologic, histochemical transcriptomic analyses. We show that productively infects modulates vascular responses arteries. This finding has far reaching implications for the understanding clinical treatment COVID-19.