A5086144836- Renal and related cancers
- Renal cell carcinoma treatment
- Pluripotent Stem Cells Research
- Renal Transplantation Outcomes and Treatments
- Cancer, Hypoxia, and Metabolism
- Polyamine Metabolism and Applications
- Ethics and Legal Issues in Pediatric Healthcare
- Amino Acid Enzymes and Metabolism
- Gene expression and cancer classification
- Genetic and Kidney Cyst Diseases
- Genetics and Neurodevelopmental Disorders
- Erythropoietin and Anemia Treatment
- Gene Regulatory Network Analysis
- Muscle metabolism and nutrition
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Congenital heart defects research
- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- Cannabis and Cannabinoid Research
- RNA regulation and disease
- Prenatal Screening and Diagnostics
- Bioinformatics and Genomic Networks
Charité - Universitätsmedizin Berlin
2014-2024
Humboldt-Universität zu Berlin
2018-2023
Freie Universität Berlin
2018-2023
RELX Group (United States)
2023
Baylor College of Medicine
2022
University of Zurich
2022
Boston Children's Hospital
2022
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2020
Candy’s Place
2014
Schmerzmedizin Berlin
2007
Nestin is an intermediate filament protein originally described in neural stem cells and a variety of progenitor cells. More recently, nestin was detected rat kidney podocytes. We show here that expressed developmentally regulated pattern the kidney. by immunohistochemistry condensing mesenchyme surrounding ureter, developing glomeruli, podocytes adult kidney, podocyte cell line. shared striking overlap expression with Wilms' tumor suppressor Wt1. significantly upregulated line inducible Wt1...
Neuronal lineage formation in the developing olfactory epithelium has been extensively studied at cellular level, but little is known about genes that control proliferation and differentiation of neuronal progenitor cells. Here, we report Wilms' tumour zinc-finger protein, Wt1, required for normal epithelium. Wt1 was detected by immunohistochemistry wild-type embryos between gestational days E9.5 E18.5. Embryos with complete lack selective ablation alternatively spliced Wt1(+KTS) isoform...
Neurotrophins and their cognate receptors play a pivotal role in the development function of nervous system. High expression levels neurotrophin receptor TrkB its ligands neuroblastomas are associated with an unfavorable outcome. We report here that NTRK2, which encodes tyrosine kinase, is oxygen-regulated gene, whose stimulated by hypoxia-inducible factor-1 (HIF-1). mRNA protein were elevated nearly 30-fold neuroblastoma-derived Kelly cells hypoxia (1% O2) versus normoxia (21% O2). A...
Current immunosuppressive strategies in organ transplantation rely on calcineurin inhibitors cyclosporine A (CsA) or tacrolimus (Tac). Both drugs are nephrotoxic, but CsA has been associated with greater renal damage than Tac. inhibits by forming complexes cyclophilins, whose chaperone function is essential for proteostasis. We hypothesized that stronger toxicity of may be related to suppression cyclophilins ensuing endoplasmic reticulum (ER) stress and unfolded protein response (UPR) kidney...
Fragile X syndrome is a common inherited cause of mental retardation that results from loss or mutation the fragile protein (FMRP). In this study, we identified mRNA basic helix-loop-helix transcription factor human achaete-scute homologue-1 (hASH1 ASCL1), which required for normal development nervous system and has been implicated in formation neuroendocrine tumors, as new FMRP target. Using double-immunofluorescent staining technique detected an overlapping pattern both proteins...
Adequate tissue oxygenation is a prerequisite for normal development of the embryo. Most fetal organs are exquisitely susceptible to hypoxia which occurs when delivery oxygen exceeded by actual demand. Developmental abnormalities due insufficient supply with can result from impaired expression genes essential functions during embryogenesis. As such, Wilms' tumor gene, WT1, among that regulated local tension. WT1 was originally discovered as suppressor gene owing loss-of-function mutations in...
IntroductionCongenital anomalies of the kidney and urinary tract (CAKUT) represent most common cause chronic disease in children. While only 20% cases can be genetically explained, majority remain without an identified underlying etiology. The neurodevelopmental disorder Chung-Jansen syndrome (CHUJANS) is caused by haploinsufficiency Pleckstrin homology domain-interacting protein (PHIP) was previously associated with genital malformations. Anecdotal coincidence CHUJANS CAKUT prompted us to...
The Wilms' tumor protein Wt1 is required for embryonic development and has been implicated in hematologic disorders. Since deficiency may compromise the proliferation differentiation of erythroid progenitor cells, we analyzed possible role transcriptionally active isoform, Wt1(-KTS), regulating expression erythropoietin receptor (EpoR). EpoR were coexpressed CD117(+) hematopoietic cells several cell lines. Wt1-deficient murine embryos (Wt1(-/-)) exhibited a significantly lower response to...
We identified the Wilms’ tumor protein, Wt1, as a novel transcriptional activator of immunosuppressant cytokine interleukin‐10 (IL‐10). Silencing Wt1 by RNA interference reduced IL‐10 mRNA levels approximately 90%. transcripts were increased more than 15‐fold upon forced expression Wt1. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed cis ‐element that was responsible for activation promoter in murine macrophages. Mutation binding motif abrogated stimulation...
Scaffolding proteins play pivotal roles in the assembly of macromolecular machines such as spliceosome. The adaptor protein CD2BP2, originally identified a binding partner adhesion molecule CD2, is pre-spliceosomal factor that utilizes its glycine-tyrosine-phenylalanine (GYF) domain to co-localize with spliceosomal proteins. So far, function vertebrates unknown. Using conditional gene targeting mice, we show CD2BP2 crucial for embryogenesis, leading growth retardation, defects...
Gonad morphogenesis relies on the correct spatiotemporal expression of a number genes that together fulfill differentiation bipotential gonad into testes or ovaries. As such, transcription factors WT1 and GATA4 are pivotal for proper gonadal development. Here we address contributions to sex phase in We applied an ex vivo technique cultivating gonads hanging droplets media were supplemented with vivo-morpholinos knockdown either alone combination at same developmental stage. show is equally...
Wilms tumor protein 1 (WT1) has been implicated in the control of several genes sexual development, but its function gonad formation is still unclear. Here, we report that WT1 stimulates expression Kdr, gene encoding VEGF receptor 2, murine embryonic gonads. We found and KDR are co-expressed Sertoli cells testes somatic ovaries. Vivo-morpholino–mediated knockdown decreased Kdr transcripts cultured gonads at multiple developmental stages. Furthermore, bound to promoter chromatin Forced...
Background/Aims: Wilms′ tumour protein, Wt1, is a zinc finger molecule, which required for normal embryonic development. Mutations of the WT1 gene can give rise to childhood cancer kidneys. Different Wt1 isoforms exist, function either as transcription factors or have presumed role in mRNA processing. Previous studies suggested that undergoes nucleocytoplasmic shuttling, and cytoplasmic was higher malignant than cells. The aim this study analyse molecular pathways along shuttles between...
Abstract Aim In acute kidney injury ( AKI ), regions of the are hypoxic. However, for reasons yet unknown, adaptation to hypoxia through hypoxia‐inducible factor HIF ) is limited. Here, we studied miR‐22, a potential repressor, in normal kidneys, as well rhabdomyolysis‐induced , condition where miR‐22 up‐regulated. Methods mice was provoked by IM injection glycerol. Tissue homogenates were processed determine levels candidate RNA s and proteins, global gene expression profiles. Reporter...