A5103138143- Renal Transplantation Outcomes and Treatments
- RNA Interference and Gene Delivery
- Oral and gingival health research
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Organ Transplantation Techniques and Outcomes
- Magnesium in Health and Disease
- Signaling Pathways in Disease
- Biomedical Research and Pathophysiology
- Ion Transport and Channel Regulation
- Fungal and yeast genetics research
- Electrospun Nanofibers in Biomedical Applications
- Enzyme Catalysis and Immobilization
- Pharmacological Effects and Toxicity Studies
- Genetic and Kidney Cyst Diseases
- Kidney Stones and Urolithiasis Treatments
- Cerebral Palsy and Movement Disorders
- Histone Deacetylase Inhibitors Research
- Thermoregulation and physiological responses
- Bee Products Chemical Analysis
- Polyomavirus and related diseases
- PARP inhibition in cancer therapy
- Renal and Vascular Pathologies
- Musculoskeletal pain and rehabilitation
- Medical and Biological Ozone Research
Charité - Universitätsmedizin Berlin
2018-2025
Hacettepe University
2024
Yeditepe University
2024
Medicines Discovery Catapult
2022
Marmara University
2014-2015
Middle East Technical University
2014
Institute of Molecular Life Sciences
2014
Delft University of Technology
2011-2012
A wide variety of phytochemicals are consumed for their perceived health benefits. Many these have been found to alter numerous cell functions, but the mechanisms underlying biological activity tend be poorly understood. Phenolic particularly promiscuous modifiers membrane protein function, suggesting that some actions may due a common, bilayer-mediated mechanism. To test whether bilayer perturbation underlie this diversity actions, we examined five bioactive phenols reported medicinal...
Nanofibers have high potential through their porosity, small pore sizes, lightweight materials, and ability to mimic the extracellular matrix structure for use in manufacture of wound dressings treatment. In this study, poly(lactic-co-glycolic acid) (PLGA) nanofibers were produced by electrospinning. Propolis was loaded into PLGA dropping method. The average diameters effects propolis loading on morphology 37.5, 50, 100% propolis-loaded (PLGA-P37.5, PLGA-P50, PLGA-P100) evaluated scanning...
Significance Understanding the working mechanism of membrane proteins is difficult even when crystal structures are available. One promising approach ion mobility–mass spectrometry (IM-MS) that detects not only mass-to-charge ratio but also area by measuring rotationally averaged collision cross-sections (CCS) in gas phase. We identified detergents allow release at low levels collisional activation for native MS, thus avoiding denaturing effects. studied gating an channel, which occurs...
Current immunosuppressive strategies in organ transplantation rely on calcineurin inhibitors cyclosporine A (CsA) or tacrolimus (Tac). Both drugs are nephrotoxic, but CsA has been associated with greater renal damage than Tac. inhibits by forming complexes cyclophilins, whose chaperone function is essential for proteostasis. We hypothesized that stronger toxicity of may be related to suppression cyclophilins ensuing endoplasmic reticulum (ER) stress and unfolded protein response (UPR) kidney...
Calcineurin inhibitors (CNIs) are the backbone for immunosuppression after solid organ transplantation. Although successful in preventing kidney transplant rejection, their nephrotoxic side effects contribute to allograft injury. Renal parenchymal lesions occur cyclosporine A (CsA) as well currently favored tacrolimus (Tac). We aimed study whether chronic CsA and Tac exposures, before reaching irreversible damage, affect renal compartments differentially related pathogenic mechanisms can be...
Kidney thick ascending limb cells reabsorb sodium, potassium, calcium, and magnesium contribute to urinary concentration. These are typically viewed as of a single type that recycles potassium across the apical membrane generates lumen-positive transepithelial voltage driving calcium reabsorption, although variability in channel expression has been reported. Additionally, recent transcriptomic analyses suggest different cell types exist along this segment, but classifications have varied not...
Abstract Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent genetic disorder, affecting over 10 million individuals worldwide. Cystic expansion typically progresses to failure and also involves liver with limited treatment options. Pathogenic variants in PKD1 or PKD2 account for 85-90% of cases. Genetic re-expression Pkd1 Pkd2 has been shown partially reverse key characteristics phenotype mice. Despite advancements understanding basis, it remains unclear whether...
Quercetin, resveratrol, naringenin, hesperidin, and rutin are phenolic compounds/flavonoids that may have roles in the reduction of cancer susceptibility. In this study, vitro modulatory effects them were studied on liver CYP1A1 associated 7-ethoxyresorufin-O-deethylase (EROD) activity glutathione S-transferase (GST) leaping mullet (Liza saliens). All used exerted an inhibitory effect both EROD GST activities fish. found to inhibit a competitive manner; other hand, naringenin was...
Significance Statement XBP1 activation in neonatal and adult doxycycline-inducible murine models of ADPKD due to a hypomorphic polycystin-1 missense mutation orthologous human PC1R2220W delays cyst formation. Activating XBP1s, pro-chaperone inducer the endoplasmic reticulum stress response, can improve steady-state expression, ciliary trafficking, cleavage mutant protein, providing initial vivo proof concept that modulating levels poorly functioning PC1 alleles slow progression kidney...
Abstract Aim Perturbed calcium homeostasis limits life expectancy in familial hypomagnesaemia with hypercalciuria and nephrocalcinosis (FHHNC). This rare disease occurs by loss‐of‐function mutations CLDN16 or CLDN19 genes, causing impaired paracellular reabsorption of divalent cations along the cortical thick ascending limb (cTAL). Only partial compensation takes place ensuing late distal convoluted tubule, connecting collecting duct, where luminal transient receptor potential channel V5...
Plasma membrane H(+)-ATPase isoforms with increased H(+)/ATP ratios represent a desirable asset in yeast metabolic engineering. In vivo proton coupling of two previously reported Pma1p (Ser800Ala, Glu803Gln) vitro stoichiometries was analysed by measuring biomass yields anaerobic maltose-limited chemostat cultures expressing only the different PMA1 alleles. wildtype and did not differ significantly.
Connexins (Cxs) form gap junctions for intercellular exchange of inorganic ions and messenger molecules. In the kidney, Cxs play essential roles within its compartments, but data on precise cellular localization cell type-related function their isoforms are scarce. We tested whether Cx43 distribution is restricted to vascular interstitial cells medullary fibroblasts express coordinate profibrotic signaling. Confocal immunofluorescence techniques, ultrastructural labeling, functional...
Abstract Calcineurin inhibitors (CNI) are the backbone for immunosuppression after solid organ transplantation. Although successful in preventing kidney transplant rejection, their nephrotoxic side effects notoriously contribute to allograft injury despite attempts optimize application, often with additional medications. Complex renal parenchymal damage occurs cyclosporine A (CsA) as well currently favoured tacrolimus (Tac). To test distinct CsA and Tac damaging patterns, we combined...
Aim: Impaired calcium homeostasis limits life expectancy and quality in familial hypomagnesaemia with hypercalciuria nephrocalcinosis (FHHNC). This rare disease is caused by loss-of-function mutations CLDN16 or CLDN19 genes leading to impaired paracellular reabsorption of divalent cations along the cortical thick ascending limb (cTAL). The ensuing late distal nephron collecting duct system partially compensate for defect cTAL increased transcellular Ca 2+ via luminal transient receptor...