A5101534569- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Immunodeficiency and Autoimmune Disorders
- Trypanosoma species research and implications
- RNA and protein synthesis mechanisms
- Vector-Borne Animal Diseases
- Yersinia bacterium, plague, ectoparasites research
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Spaceflight effects on biology
- Microbial Metabolites in Food Biotechnology
- Protein Kinase Regulation and GTPase Signaling
- Dermatological and COVID-19 studies
- Diabetes and associated disorders
- Cardiomyopathy and Myosin Studies
- Chemical Synthesis and Analysis
- Insect symbiosis and bacterial influences
- Helicobacter pylori-related gastroenterology studies
- Salmonella and Campylobacter epidemiology
- Health and Medical Studies
Leibniz Supercomputing Centre
2025
University of Veterinary Medicine Vienna
2008-2020
Heidelberg University
2009-2016
University of California, San Francisco
2012
Klinik und Poliklinik für Nuklearmedizin
2010
European Molecular Biology Laboratory
2005-2007
Max Planck Institute of Biochemistry
2005
Max Delbrück Center
2005
University of Auckland
2005
UCLouvain
2001-2002
Listeria monocytogenes causes rhombencephalitis in humans and animals also affects the fetus utero , causing disseminated sepsis. In both instances, infection occurs by crossing of endothelial cells lining a physiological barrier, blood–brain barrier or transplacental barrier. this study, ability L. wild‐type EGD to invade human umbilical vein (HUVECs) was evaluated using bacteria isogenic mutants. Here, we show that invasion HUVECs is dependent on expression internalin B gene product. This...
Tyrosine kinase 2 (TYK2) is a member of the Janus (JAK) family and involved in cytokine signalling. In vitro analyses suggest that TYK2 also has kinase-independent, i.e., non-canonical, functions. We have generated gene-targeted mice harbouring mutation ATP-binding pocket domain. The Tyk2 kinase-inactive (Tyk2K923E) are viable show no gross abnormalities. kinase-active required for full-fledged type I interferon- (IFN) induced activation transcription factors STAT1-4 vivo antiviral defence...
Summary The Ysc type III secretion system allows Yersinia enterocolitica to translocate virulence proteins, called Yop effectors, into the cytosol of eukaryotic cells. Some effectors possess an individual chaperone a Syc protein. first 15 amino acids YopE effector constitute signal that is sufficient promote several reporter proteins. Residues 15–50 comprise minimal binding domain for SycE chaperone. In this study, we investigated by YopE–DHFR hybrid proteins with different folding...
A role of CD4+ T cells during the progression from nonalcoholic fatty liver disease (NAFLD) to steatohepatitis (NASH) has been suggested, but which polarization state these characterizes this and development fibrosis remain unclear. In addition, a gut-liver axis suggested play in NASH, just begun be investigated. Combining single-cell RNA sequencing multiple-parameter flow cytometry, we provide first cell atlas our knowledge focused on liver-infiltrating patients with NAFLD showing that NASH...
The transcription factor STAT1 is essential for interferon (IFN)-mediated immunity in humans and mice. function tightly regulated, both loss- gain-of-function mutations result severe immune diseases. two alternatively spliced isoforms, STAT1α STAT1β, differ with regard to a C-terminal transactivation domain, which absent STAT1β. STAT1β considered be transcriptionally inactive competitive inhibitor of STAT1α. To investigate the functions isoforms vivo, we generated mice deficient either or As...
Trypanosomosis is a serious cause of reduction in productivity cattle tsetse-fly infested areas. Baoule and other local Taurine breeds Burkina Faso are trypanotolerant. Zebuine cattle, which also kept there susceptible to trypanosomosis but bigger body size. Farmers have continuously been intercrossing Zebu animals increase production disease tolerance. The aim this study was compare levels zebuine taurine admixture genomic regions potentially involved trypanotolerance with background...
// Theophilos Tzaridis 1 , Till Milde 2, 3, 4 Kristian W. Pajtler 1, Sebastian Bender David T. Jones Simone Müller 5 Andrea Wittmann Magdalena Schlotter 6 Andreas E. Kulozik 2 Peter Lichter V. Collins 7 Olaf Witt Marcel Kool Andrey Korshunov 8, 9 Stefan M. Pfister Hendrik Division of Pediatric Neurooncology (B062), German Cancer Consortium (DKTK), Research Center (DKFZ), Heidelberg, Germany Department Oncology, Hematology and Immunology, Children's Hospital, University 3 Clinical Cooperation...
When murine peritoneal macrophages were loaded in vitro with Plasmodium vinckei hemozoin and stimulated opsonized zymosan for 90 min or lipopolysaccharide and/or interferon-gamma 24 hr, significant decreases the production of oxygen radicals nitrogen oxides, respectively, could be detected by comparison without hemozoin. Moreover, nonradioactive situ hybridization immunohistologic analysis liver sections P. vinckei-infected mice more than 60% parasitemia showed that cells still expressing...
This study was conducted to (i) determine the prevalence of African Animal Trypanosomosis (AAT) in tsetse challenged areas, (ii) compare conventional with qPCR detection systems and (iii) evaluate host genetic background biology as risk factors. AAT studies are often confronted low levels parasitaemia. Hence, we designed a novel assay using primers species specific probes amplifying Internal Transcribed Spacer 1 (ITS1) gene. Thereby all three could be detected simultaneously. 368 individuals...
AbstractSeveral pathogenic Gram-negative bacteria, including Salmonella, Shigella, Yersinia, Pseudomonas aeruginosa and enteropathogenic Escherichia coli harbour a complex attack system called 'Type III secretion' which is, in every case, an essential virulence determinant. This system, activated by contact with eukaryotic cell membrane, allows bacteria to inject bacterial proteins across the two membranes reach cell's cytosol destroy or subvert host cell. The Type mechanism consists of...
ABSTRACT The aim of this study was to investigate the effect Helicobacter pylori on function gastric mucous cells. H. (10 4 10 7 CFU/well) incubated with mucin-producing cell line HM02 for 12 and 24 h. Mucin synthesis secretion were determined by incorporation d - N -[ acetyl 14 C]glucosamine into intracellular released high-molecular-weight glycoproteins. cagA -positive, cytotoxin-producing non-cytotoxin-producing strains impaired Significant inhibition mucin noted after h cocultivation a...
The scaffold protein NBR1 is involved in signal transmission downstream of the serine/protein kinase from giant muscle titin. Its N‐terminal Phox and Bem1p (PB1) domain plays a critical role mediating protein–protein interactions with both titin another protein, p62. We have determined crystal structure PB1 at 1.55 Å resolution. It reveals type‐A two negatively charged residue clusters. provide structural perspective on involvement signalling pathway.
In patients with severe quadriplegic cerebral palsy and painful hip dislocation proximal femoral resection arthroplasty can reduce pain, but the risk of heterotopic ossification is significant. We present a surgical technique autologous capping stump in order to this risk, using resected head as graft. A retrospective study 31 (43 hips) who had undergone (29 without (14 was undertaken. Heterotopic less frequent capping, more predictable pattern bony overgrowth found. For selected group...