A5061240330- Ruminant Nutrition and Digestive Physiology
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Diet and metabolism studies
- Gout, Hyperuricemia, Uric Acid
- Probiotics and Fermented Foods
- Genetic and phenotypic traits in livestock
- Nutritional Studies and Diet
- Cancer-related molecular mechanisms research
- Animal Nutrition and Physiology
- Turfgrass Adaptation and Management
- Clostridium difficile and Clostridium perfringens research
- Circular RNAs in diseases
- Microbial infections and disease research
- Plant and fungal interactions
- Bacteriophages and microbial interactions
- Gastrointestinal disorders and treatments
- Identification and Quantification in Food
- Zoonotic diseases and public health
- Animal health and immunology
- Immune cells in cancer
- Pharmaceutical and Antibiotic Environmental Impacts
- Histone Deacetylase Inhibitors Research
- Cancer Mechanisms and Therapy
- Insect symbiosis and bacterial influences
Zhejiang University
2024-2025
South China Agricultural University
2023-2025
University of Alberta
2016-2025
City University of Hong Kong
2021-2024
Food & Nutrition
2024
China Medical University
2018-2022
Soochow University
2022
Liaoning Provincial People's Hospital
2014-2021
Jinan City People's Hospital
2020
Shandong First Medical University
2020
The symbiotic rumen microbiota is essential for the digestion of plant fibers and contributes to variation production health traits in ruminants. However, date, heritability microbial features host genetic components associated with microbiota, as well whether such are animal performance relevant, largely unknown. In present study, we assessed from a cohort 709 beef cattle showed that multiple factors including breed, sex, diet drove among animals. diversity indices, relative abundance ~ 34%...
Exploring compositional and functional characteristics of the rumen microbiome can improve understanding its role in function cattle feed efficiency. In this study, we applied metatranscriptomics to characterize active microbiomes beef with different efficiencies (efficient, n = 10; inefficient, 10) using total RNA sequencing. Active bacterial archaeal compositions were estimated based on 16S rRNAs, microbial metabolic functions including carbohydrate-active enzymes (CAZymes) assessed mRNAs...
Microorganisms are responsible for fermentation within the rumen and have been reported to contribute variation in feed efficiency of cattle. However, what extent breed affects microbiome its association with host is unknown. Here, microbiomes beef cattle (n = 48) from three breeds (Angus, Charolais, Kinsella composite hybrid) high low were explored using metagenomics metatranscriptomics, aiming identify differences between functional potentials activities same evaluate effects on...
Inflammation-associated insulin resistance is a key trigger of gestational diabetes mellitus (GDM), but the underlying mechanisms and effective interventions remain unclear. Here, we report association placental inflammation (tumor necrosis factor–α) abnormal maternal glucose metabolism in patients with GDM, high fermentable dietary fiber (HFDF; konjac ) could reduce GDM development through gut flora–short-chain fatty acid–placental axis mouse model. Mechanistically, HFDF increases...
Industrialization adversely affects the gut microbiome and predisposes individuals to chronic non-communicable diseases. We tested a restoration strategy comprising diet that recapitulated key characteristics of non-industrialized dietary patterns (restore diet) bacterium rarely found in industrialized microbiomes (Limosilactobacillus reuteri) randomized controlled feeding trial healthy Canadian adults. The restore diet, despite reducing diversity, enhanced persistence L. reuteri strain from...
Ten strains, BG-AF3-AT, pH52_RY, WF-MT5-AT, BG-MG3-A, Lr3000T, RRLNB_1_1, STM3_1T, STM2_1, WF-MO7-1T and WF-MA3-C, were isolated from intestinal or faecal samples of rodents, pheasant primate. 16S rRNA gene analysis identified them as Limosilactobacillus reuteri. However, average nucleotide identity digital DNA-DNA hybridization values based on whole genomes below 95 70 %, respectively, thus the threshold levels for bacterial species delineation. Based genomic, chemotaxonomic morphological...
Dietary fiber is an integral part of a healthy diet, but questions remain about the mechanisms that underlie effects and causal contributions gut microbiota. Here, we performed 6-week exploratory trial in adults with excess weight (BMI: 25-35 kg/m2) to compare high-dose (females: 25 g/day; males: 35 g/day) supplement fermentable corn bran arabinoxylan (AX; n = 15) microbiota-non-accessible microcrystalline cellulose (MCC; 16). Obesity-related surrogate endpoints biomarkers host-microbiome...
It is unclear if coexistence theory can be applied to gut microbiomes understand their characteristics and modulate composition. Through experiments in gnotobiotic mice with complex microbiomes, we demonstrated that strains of Akkermansia muciniphila Bacteroides vulgatus could only established were devoid these species. Strains A. showed strict competitive exclusion, while B. coexisted but populations still influenced by interactions. These differences behavior reflective genomic variation...
Abstract Background Structural variations (SVs) in individual genomes are major determinants of complex traits, including adaptability to environmental variables. The Mongolian and Hainan cattle breeds East Asia taurine indicine origins that have evolved adapt cold hot environments, respectively. However, few studies investigated SVs Asian their roles adaptation, little is known about adaptively introgressed cattle. Results In this study, we examine the climate adaptation these two lineages...
Abstract Background Gut microbes play crucial roles in the development and health of their animal hosts. However, evolutionary relationships gut with vertebrate hosts, consequences that arise for ecology lifestyle are still insufficiently understood. Specifically, mechanisms by which strain-level diversity evolved, degree lineages remain stably associated how history influences ecological performance a critical gap our understanding vertebrate-microbe symbiosis. Results This study presents...
Emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) poses grave threats to human health. These strains increased dramatically in clinical settings China the past few years but not other parts world. Four isogenic K. strains, including classical pneumoniae, (CRKP), (hvKP) and CR-hvKP, were created subjected phenotypic characterization, competition assays, mouse sepsis model rat colonization tests investigate mechanisms underlying wide spread CR-hvKP China....
Taxonomic characterization of active gastrointestinal microbiota is essential to detect shifts in microbial communities and functions under various conditions. This study aimed identify quantify potentially rumen using total RNA sequencing compare the outcomes this approach with widely used targeted RNA/DNA amplicon technique. Total isolated from digesta samples five beef steers was subjected Illumina paired-end (RNA-seq), bacterial archaeal amplicons partial 16S rRNA/rDNA were 454...
Through experiments in gnotobiotic mice that employed isogenic mutants of bacterial strains produce ( Bifidobacterium breve ) and utilize Lactobacillus reuteri 1,2-propanediol, this study provides mechanistic insight into the ecological ramifications a trophic interaction between gut symbionts. The findings improve our understanding on how cross-feeding influences competitive fitness L. vertebrate revealed putative selective force shaped evolution species. are relevant since they provide...
The gut microbiota makes critical contributions to host homeostasis, and its role in the treatment of acute myeloid leukaemia (AML) has attracted attention. We investigated whether microbiome is affected by AML, such changes are associated with cachectic hallmarks. Biological samples clinical data were collected from 30 antibiotic-free AML patients at diagnosis matched volunteers (1:1) a multicenter cross-sectional prospective study. composition functional potential faecal analyzed using...
The advent of next generation sequencing and bioinformatics tools have greatly advanced our knowledge about the phylogenetic diversity ecological role microbes inhabiting mammalian gut. However, there is a lack information on evaluation these computational in context rumen microbiome as programs mostly been benchmarked real or simulated datasets generated from human studies. In this study, we compared outcomes two methods, Kraken (mRNA based) pipeline developed in-house based Mothur (16S...
This study evaluated the effect of feeding non-heated and heated colostrum on mucosa- digesta-associated microbiota in colon dairy calves during first 12 h life. Thirty-two neonatal Holstein male were fed: no (NC, n = 8), (FC, 12) (HC (60 °C, 60 min), immediately after birth. The abundances total bacteria higher FC fed compared to those (NC) at Compare NC calves, a proportion Clostridium cluster XIVa Bifidobacterium, lower abundance mucosa E. coli detected HC as well tentatively relative...
Exploring the compositional characteristics of rumen eukaryotic community can expand our understanding their role in function and feed efficiency. In this study, we applied metatranscriptomics to characterize active (protozoa fungi) beef cattle (n = 48) three breeds [Angus (AN), Charolais (CH), Kinsella Composite (KC)] with divergent residual intake (RFI). The composition microbiota was evaluated based on enriched 18S rRNAs from metatranscriptomic datasets. At phylum level, a total 4...
Abstract Glioblastoma multiforme (GBM) brings serious physical and psychological pain to GBM patients, whose survival rate remains not optimistic. Long noncoding RNAs (lncRNAs) have been reported participate in the progression of many cancers, including GBM. However, mechanism function long intergenic non-protein coding RNA 1152 (LINC01152) are still unclear. In our study, we aimed explore LINC01152 Then qRT-PCR analysis was implemented search expression tissues cells. Functional assays such...
The mammalian microbiome encodes numerous secondary metabolite biosynthetic gene clusters; yet, their role in microbe-microbe interactions is unclear. Here, we characterized two polyketide synthase clusters (fun and pks) the gut symbiont Limosilactobacillus reuteri. pks, but not fun, cluster antimicrobial activity. Forty-one of 51 L. reuteri strains tested are sensitive to Pks products; this finding was independent strains' host origin. Sensitivity also established intraspecies competition...
Sows are highly sensitive to deoxynivalenol (DON) and susceptible reproductive toxicity caused by oxidative stress, but the potential mechanisms effective interventions remain unclear. Here, we investigated role of two antioxidants (cysteamine N-acetyl-cysteine) in regulating performance, redox status, placental barrier function sows their under DON exposure. Maternal dietary supply from day 85 gestation parturition reduced incidence stillbirths low-birth-weight piglets Moreover, alleviation...