A5027725820- Gut microbiota and health
- Transplantation: Methods and Outcomes
- Probiotics and Fermented Foods
- Clostridium difficile and Clostridium perfringens research
- Folate and B Vitamins Research
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Zoonotic diseases and public health
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Genomics and Phylogenetic Studies
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Diet and metabolism studies
- Identification and Quantification in Food
- Xenotransplantation and immune response
- Organ Transplantation Techniques and Outcomes
- Tryptophan and brain disorders
- Orthopedic Infections and Treatments
- Biochemical Analysis and Sensing Techniques
- RNA Interference and Gene Delivery
- Escherichia coli research studies
- Child Nutrition and Water Access
University of Alberta
2013-2025
Abstract Background Gut microbes play crucial roles in the development and health of their animal hosts. However, evolutionary relationships gut with vertebrate hosts, consequences that arise for ecology lifestyle are still insufficiently understood. Specifically, mechanisms by which strain-level diversity evolved, degree lineages remain stably associated how history influences ecological performance a critical gap our understanding vertebrate-microbe symbiosis. Results This study presents...
Through experiments in gnotobiotic mice that employed isogenic mutants of bacterial strains produce ( Bifidobacterium breve ) and utilize Lactobacillus reuteri 1,2-propanediol, this study provides mechanistic insight into the ecological ramifications a trophic interaction between gut symbionts. The findings improve our understanding on how cross-feeding influences competitive fitness L. vertebrate revealed putative selective force shaped evolution species. are relevant since they provide...
Although identifying specific microbial taxa associated with obesity and type 2 diabetes remains difficult, certain bacteria may play an important role in initiating metabolic inflammation during disease development. Here, we used a mouse model distinguishable by the presence or absence of commensal Escherichia coli strain combination high-fat diet challenge to investigate impact E. on host outcomes.
The gut microbiota harbors a diverse phage population that is largely derived from lysogens, which are bacteria contain dormant phages in their genome. While the diversity of ecosystems getting increasingly well characterized, knowledge limited on how contribute to evolution and ecology host bacteria. Here, we show biologically active prophages widely distributed phylogenetically strains symbiont Lactobacillus reuteri Nearly all human- rodent-derived strains, but less than half tested...
Abstract Background Vitamin B12 supplements typically contain doses that far exceed the recommended daily amount, and high exposures are generally considered safe. Competitive syntrophic interactions for exist between microbes in gut. Yet, to what extent excessive levels contribute activities of gut microbiota remains unclear. The objective this study was evaluate effect on microbial ecology using a supplemented mouse model with Citrobacter rodentium , mouse-specific pathogen. Mice were fed...
The establishment of microbial communities in neonatal mammals plays a pivotal role shaping their immune responses to infections and other immune-related conditions. This process is influenced by combination endogenous exogenous factors. Previously, we reported that depletion CD71 + erythroid cells (CECs) results an inflammatory response newborn mice. Here, systemically tested this hypothesis observed the small intestinal lamina propria mice had highest frequency CECs during early days life....
Background: Development and maturation of the immune system begin in utero continue throughout neonatal period. Both maternal gut microbiome influence development, but relative importance prenatal postnatal periods is unclear. Methods: In present study, we characterized cell populations mice which timing colonization was strictly controlled using gnotobiotic methodology. Results: Compared to conventional (CONV) mice, germ-free (GF) conventionalized at birth (EC mice) showed few differences...
The immature immune system is uniquely susceptible to tolerance induction and thus an attractive target for immunomodulation strategies organ transplantation. Newborn mice injected with adult semi-allogeneic lymphohematopoietic cells accept transplants without immunosuppressive drugs. Early in vivo/in situ events leading neonatal remain poorly understood. Here, we show by whole body/organ imaging that home lymphoid organs liver where various F1-donor cell types selectively alter immunity. In...
Egg white protein ovomucin and its hydrolysates were previously reported to exhibit anti-inflammatory anti-adhesive activities. However, their potential regulate pathogen colonization disease severity has not been fully characterized. To investigate the effects of (OVM) including ovomucin-Protex 26L (OP) -pepsin/pancreatin (OPP) on host resistance infection, a well-documented colitis model in mice for attaching effacing E. coli pathogens, Citrobacter rodentium, was used current study....
Introduction: Polysaccharides are considered TI antigens capable of eliciting antibody secretion without T-cell help. TNP-Ficoll is used as a model (type 2) antigen, but detailed mechanisms B-cell responses remain unclear. We studied role in the response to polysaccharides, using clinically relevant ABO antigen its natural form. Methods: Mice (4-8 week-old) received three standardized immunizations (days 0- 7- 14) with human blood group-A erythrocyte membranes, or sperm whale myoglobin...
Abstract Background: Vitamin B12 supplements typically contain doses that far exceed the recommended daily amount, and high exposures are generally considered safe. Competitive syntrophic interactions for exist between microbes on gut. Yet, it has to be established what extent excessive levels affect gut microbiota infection resistance. The objective of this study was evaluate effect excess microbial ecology pathogen virulence. Mice were fed a standard chow diet received either water or...
Abstract The mucus layer is critical to gastrointestinal health and ecology. Dietary phytochemicals are well documented stimulate production secretion, but the underlying mechanism effects on gut poorly understood. We fed germ-free conventional mice diets containing approximately 0.4% of polyphenols per gram determine if phytochemical-induced accumulation mucin in lumen dependent microbiota. In addition, we assess how increased shapes microbial communities mice. Germ-free receiving a pea (...
Introduction: Newborn mice injected with a mixture of semi-allogeneic (F1) bone marrow (BMC) and spleen cells (SC) accept donor-type transplants as adults. B are abundant in both BMC SC have been reported to tolerize CD8+ T cells. To determine what role, if any, the tolerizing inoculum play this classic tolerance model, we studied effect purified into newborn mice. Methods: F1-B allogeneic-B (allo-B) were from using StemCell Technologies EasySep® CD19 positive selection kits. C3H/He (C3H;...
Introduction: Histo-blood group ABH(O) ags are oligosaccharides present as glycolipids or glycoproteins. A, B H epitopes carried by 6 different internal carbohydrate backbones (type I-VI), forming antigenically distinct variants, each type differentially expressed in human tissues. In heart, only II structures (on vascular endothelium) whereas red cells express I-IV. Identifying natural antibody (ab) clones specific to these ABH may be important the setting of ABOi transplantation. Our...