A5091469299- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Cancer therapeutics and mechanisms
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Probiotics and Fermented Foods
- vaccines and immunoinformatics approaches
- Antibiotic Use and Resistance
- Phenothiazines and Benzothiazines Synthesis and Activities
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
Hong Kong Polytechnic University
2014-2025
Shenzhen Polytechnic
2023-2024
City University of Hong Kong
2020-2023
Hefei University of Technology
2017
The epidemiological features of the newly emerged carbapenem-resistant hypervirulent
Abstract Detailed genomic and structure-based analysis of a new coronavirus, namely 2019-nCoV, showed that the virus is type bat coronavirus genetically fairly distant from human SARS coronavirus. Structure spike (S) protein this its S only binds weakly to ACE2 receptor on cells whereas exhibits strongly affinity ACE receptor. These findings suggest does not readily transmit between humans should theoretically able cause very serious infection. data are important guide design infection...
Carbapenem-resistant and hypervirulent K. pneumoniae (CR-HvKP) strains that have emerged recently caused infections of extremely high mortality in various countries. In this study, we discovered a conjugative plasmid encodes carbapenem resistance hypervirulence clinical ST86 K2 CR-HvKP, namely 17ZR-91. The (p17ZR-91-Vir-KPC) was formed by fusion non-conjugative pLVPK-like blaKPC-2-bearing is present dynamically with two other non-fusion plasmids. Conjugation p17ZR-91-Vir-KPC to enabled them...
Acinetobacter baumannii is one of the key Gram-negative pathogens that can cause serious nosocomial infections. In China, a large proportion clinical A. strains are multidrug resistant, among which resistant to carbapenems particularly worrisome, as infections caused by such may limit choice existing antibiotics. We conducted nationwide and genome-based surveillance on prevalence antibiotic susceptibility profile carbapenem-resistant (CRAB) collected from intensive care units (ICUs) in...
Emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) poses grave threats to human health. These strains increased dramatically in clinical settings China the past few years but not other parts world. Four isogenic K. strains, including classical pneumoniae, (CRKP), (hvKP) and CR-hvKP, were created subjected phenotypic characterization, competition assays, mouse sepsis model rat colonization tests investigate mechanisms underlying wide spread CR-hvKP China....
Fluoroquinolone resistance in Salmonella has become increasingly prevalent recent years. To probe the molecular basis of this phenomenon, genetic and phenotypic features fluoroquinolone resistant strains isolated from food samples were characterized. Among 82 tested, rate three front line antibiotics ceftriaxone, ciprofloxacin azithromycin was 10%, 39% 25% respectively, which is significantly higher than that reported other countries. Ciprofloxacin typically exhibited cross-resistance to...
The recently discovered colistin resistance-encoding element, mcr-1, adds to the list of mobile resistance genes whose products rapidly erode antimicrobial efficacy not only commonly used antibiotics, but also last line agents carbapenems and colistin. relative prevalence mcr-1-bearing strains in various ecological niches including 1,371 food samples, 480 animal faecal 150 human samples 34 water was surveyed using a novel in-house method. Bacteria bearing mcr-1 were detected (71% samples),...
The rapid dissemination of non-conjugative virulence plasmids among non-K1/K2 types Klebsiella pneumoniae poses an unprecedented threat to human health, yet the underlying mechanisms governing such is unclear. In this study, a novel 68 581 bp IncFIA plasmid discovered that can be fused hypervirulence-encoding form hybrid conjugative in conjugation experiments; fusion events involve homologous recombination between 241 region located each two plasmids. conjugated both classic and blaKPC-2...
The emergence of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) causing high mortality in clinical patients infers the urgent need for developing therapeutic agents. Here, we demonstrated vitamin C (VC) exhibited strong bactericidal, anti-biofilm, and virulence-suppressing effects on CR-hvKP. Our results showed such a bactericidal effect is dose-dependent both vitro mouse infection model associated with induction reactive oxygen species (ROS) generation. In addition, VC...
The global transmission of carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant and grave threat to human health. To investigate the potential relationship between hospital sewage CRAB within healthcare facilities, isolates spp. obtained from untreated samples were subjected antimicrobial susceptibility tests, genome sequencing, bioinformatic phylogenetic tree analysis, that data matched with those clinical isolates. Among 70 tested, A. was most prevalent detectable in 5...
Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a bacterial pathogen that may cause serious drug-resistant infections are potentially fatal. To investigate the genetic characteristics of these organisms, we tested 416 P. strains recovered from 12 types clinical samples collected in 29 different hospital wards 10 hospitals Guangdong Province, China, 2017 to 2020. These were found belong 149 known sequence (STs) and 72 novel STs, indicating transmission involved multiple routes. A high...
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Development of polymyxin resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a severe challenge to public health. Here we aimed perform retrospective study prevalence and molecular characteristics polymyxin-resistant CRKP strains. 4455 clinical strains from 18 provinces China during 2000 2023 were collected. Polymyxin-resistant subjected antimicrobial susceptibility testing, whole genome sequencing bioinformatic analysis. Molecular mechanisms underlying the analyzed. show...
Klebsiella pneumoniae ( Kp ) is responsible for a wide range of infections, including pneumonia, sepsis, and urinary tract infections. However, the treatment options are limited due to continuous evolution drug-resistant hypervirulent variants. It crucial investigate mechanisms behind high mortality rate (hv strains develop new strategies preventing hv from evading host’s defenses improving effectiveness these fatal In this study, we used -induced mouse bacteremia model performed single-cell...
ABSTRACT Vibrio parahaemolyticus is an important causative agent of gastroenteritis, with the consumption contaminated seafood being major transmission route. Resistance to penicillin common among V. strains, whereas cephalosporin resistance remains rare. In attempt assess current prevalence and characteristics antibiotic this pathogen in food samples, a total 54 (17% samples) strains were isolated from 318 meat samples purchased supermarkets wet markets Shenzhen, China, 2013. These isolates...
Ciprofloxacin resistance in Salmonella has been increasingly reported due to the emergence and dissemination of multiple Plasmid-Mediated Quinolone Resistance (PMQR) determinants, which are mainly located non-conjugative plasmids or chromosome. In this study, we aimed depict molecular mechanisms underlying rare phenomenon horizontal transfer ciprofloxacin phenotype by conjugation experiments, S1-PFGE complete plasmid sequencing. Two types plasmids, namely an IncX1 type carrying a qnrS1 gene,...
Increased usage of daptomycin to treat infections caused by Gram-positive bacterial pathogens has resulted in emergence resistant mutants. In a search for more effective analogues through medicinal chemistry studies, we found that methylation at the nonproteinogenic amino acid kynurenine could result significant enhancement antibacterial activity. Termed "kynomycin," this new antibiotic exhibits higher activity than and is able eradicate methicillin-resistant Staphylococcus aureus (MRSA)...
Abstract Colistin is the last-line antibiotic against Gram-negative pathogens. Here we identify an FDA-approved drug, Otilonium bromide (Ob), which restores activity of colistin colistin-resistant bacteria in vitro and a mouse infection model. Ob also reduces dosage required for effective treatment infections caused by colistin-susceptible bacteria, thereby reducing toxicity drug regimen. Furthermore, acts synergistically with eradicating multidrug-tolerant persisters vitro. Functional...
This study aimed to characterize novel conjugative plasmids that encode transferable ciprofloxacin resistance in Salmonella . In this study, 157 nonduplicated isolates were recovered from food products, of which 55 found be resistant ciprofloxacin.
Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), two multidrug-resistant (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) predicted originate single pKPC-CR-HvKP4-like multireplicon through homologous recombination.
This study aims to investigate the prevalence and transmission dynamics of blaNDM-1 gene in animal Escherichia coli strains. Two IncFII blaNDM-1-encoding plasmids with only minor structural variation MDR region, pHNEC46-NDM pHNEC55-NDM, were found be responsible for these The can incorporated into stably inherited animal-borne E. strains that maintained gut microflora even without carbapenem selection pressure.