A5037228225- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Salmonella and Campylobacter epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Escherichia coli research studies
- Mycobacterium research and diagnosis
- Periodontal Regeneration and Treatments
- Pneumonia and Respiratory Infections
- Antimicrobial Peptides and Activities
- Tuberculosis Research and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial agents and applications
- Streptococcal Infections and Treatments
- CRISPR and Genetic Engineering
- Chromium effects and bioremediation
- Biopolymer Synthesis and Applications
- Bacterial Identification and Susceptibility Testing
- Neonatal and Maternal Infections
South China Agricultural University
2014-2024
Key Laboratory of Guangdong Province
2020-2024
Yangzhou University
2022-2024
Abstract Concerns have been raised in recent years regarding co-selection for antibiotic resistance among bacteria exposed to heavy metals, particularly copper and zinc, used as growth promoters some livestock species. In this study, 25 IncHI2 plasmids harboring oqxAB (20/25) /bla CTX-M (18/25) were found with sizes ranging from ∼260 ∼350 kb 22 belonged the ST3-IncHI2 group. addition bla , pcoA - E (5/25) silE P (5/25), well aac ( 6 ′) -Ib-cr (18/25), floR (16/25), rmtB (6/25), qnrS1 (3/25)...
IncX4 plasmids are associated with the dissemination of mcr-1 genes in Enterobacteriaceae. We screened among 2,470 isolates Enterobacteriaceae and determined positive isolates. Forty-three were observed to carry type plasmid, which 13 identified gene. Three representative mcr-1-positive selected for high-throughput sequencing. Comparative genomics showed that mcr-1-carrying exhibit remarkable similarity backbone, major distinction lies region containing mcr-1. The variable regions all fully...
Antimicrobial resistance poses a significant global challenge, demanding innovative approaches, such as the CRISPR-Cas-mediated plasmid or gene-curing system, to effectively combat this urgent crisis. To enable successful curing of antimicrobial genes plasmids through CRISPR-Cas technology, development an efficient broad-host-range delivery system is paramount. In study, we have successfully designed and constructed novel functional gene plasmid, pQ-mini, utilizing backbone Inc.Q plasmid....
4,[5],12:i:-, a monophasic variant of
In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals Guangdong province, China. The resistance-determining regions (QRDRs) gyrA and parC genes were analysed for mutations 55 harbouring only oqxAB all other PMQR genes. Overall, 282 (57.0 %) E. had at least one gene. detected 215 predominated genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 qnrB (39 isolates) qepA (18...
The current idea that compensatory evolution processes can account for the “plasmid paradox” phenomenon associated with maintenance of large costly plasmids in host bacteria has attracted much attention. Although many mutations have been discovered through various plasmid-host bacterial experiments, basis mechanisms and nature themselves to address fitness costs remain unclear.
Streptococcus suis is a zoonotic pathogen that causes disease in humans after exposure to infected pigs or pig-derived food products. In this study, we examined the serotype distribution, antimicrobial resistance phenotypes and genotypes, integrative conjugative elements (ICEs), associated genomic environments of S. isolates from China 2008 2019. We identified 13 serotypes, predominated by 2 (40/96; 41.7%), 3 (10/96; 10.4%), 1 (6/96; 6.3%). Whole-genome sequencing analysis revealed these...
The emergence of the tigecycline resistance gene tet (X) has posed a severe threat to public health. However, reports its origin and distribution in human remain rare.
The banning of colistin as a feed additive for food-producing animals in mainland China 2017 caused the decline prevalence Escherichia coli-mobilized resistance (mcr-1) China. Salmonella Typhimurium and its monophasic 1,4,[5],12:i:- variants are also main species associated with spread mcr-1; however, evidence transmission mcr-1 among is lacking. Herein, 5,354 isolates recovered from fecal samples diarrheal patients Guangdong, Southern China, 2009 to 2019 were screened mcr-1, mcr-1-positive...
Abstract Methicillin‐resistant Staphylococcus aureus (MRSA) is a current global public health problem due to its increasing resistance the most recent antibiotic therapies. One critical approach develop ways revitalize existing antibiotics. Here, we show that phytogenic compound cinnamaldehyde (CIN) and β‐lactam combinations can functionally synergize resensitize clinical MRSA isolates therapy inhibit biofilm formation. Mechanistic studies indicated CIN potentiation effect on β‐lactams was...
We report a novel fusion plasmid, pP2-3T, cointegrating sequence type 3 (ST3)-IncHI2 with an IncFII plasmid backbone mediating multidrug resistance (MDR) and virulence. Phylogenetic analysis comparative genomics revealed that pP2-3T other MDR ST3-IncHI2 plasmids clustered together, representing unique IncHI2 lineage exhibited high conservation in backbones of but possessed highly genetic plasticity various regions by acquiring numerous antibiotic genes fusing plasmids. Surveillance studies...
β-Lactam antibiotics are the mainstay for treatment of staphylococcal infections, but their utility is greatly limited by emergence and rapid dissemination methicillin-resistant Staphylococcus aureus (MRSA). Herein, we evaluated ability plant-derived monoterpene carvacrol to act as an antibiotic adjuvant, revitalizing anti-MRSA activity β-lactam antibiotics. Increased susceptibility MRSA significant synergistic activities were observed with carvacrol-based combinations. Carvacrol...
We report the complete nucleotide sequence of a plasmid carrying multiresistance gene cfr. This was isolated from an Escherichia coli strain swine origin in 2011. 37,672-bp plasmid, pSD11, had IncX4 backbone similar to those plasmids obtained United States and Australia, which cfr flanked by two copies IS26 truncated Tn1331 inserted.
During a regular monitoring of antimicrobial resistance in farrowing farm Southern China, 117 Escherichia coli isolates were obtained from sows and piglets. Compared with the piglets, exhibited higher rates to tested cephalosporins. Correspondingly, total detection rate bla CMY-2/bla CTX-M genes sow (34.2%) was also significantly than that piglet (13.6%; p < 0.05). The CMY-2 gene had relatively high prevalence (11.1%) E. isolates. MLST PFGE analysis revealed clonal spread ST1121 most (7/13)...
The emergence and spread of the novel mobile Tet(X) tetracycline destructases confer high-level tigecycline eravacycline resistance in Escherichia coli Acinetobacter spp. pose serious threats to human animal health. Therefore, a rapid robust detection assay was urgently needed monitor dissemination resistance. We developed simple detect producers Gram-negative bacteria based on matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS). This MALDITet(X) test...
To determine the transmission and molecular characteristics of blaNDM-producing Escherichia coli between companion animals their healthcare providers at veterinary clinics in Guangzhou, China.A total 359 samples from were collected 14 China. Genomic clonal relationships for blaNDM-positive E. complete plasmid sequences characterized based on WGS data combined Illumina MinION platform reads.Forty-five bacteria recovered (n = 43) 2) 10 clinics. Overall, (73.3%, 33/45) Klebsiella pneumoniae...
CRKP is one of the most important pathogens, which can cause infection not only in humans but also waterfowl. The discovery bla NDM-5 -producing K. pneumoniae waterfowl farms recent years suggests that are an reservoir for Enterobacteriaceae .
Types 1 and 3 fimbriae in Enterobacteriaceae play versatile roles bacterial physiology including attachment, invasion, cell motility as well with biofilm formation urinary tract infections. Herein, we investigated the prevalence transmission of plasmid-mediated types from 1753 non-duplicate diseased food Animals. We identified 123 (7.01%) strong producers all was E. coli. WGS analysis 43 selected revealed that they harbored multiple ARGs, ESBLs, PMQR mcr-1. The gene clusters mrkABCDF fimACDH...