Donghao Zhao

ORCID: 0000-0002-4672-9986
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About
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Research Areas
  • Turfgrass Adaptation and Management
  • Antibiotics Pharmacokinetics and Efficacy
  • Rangeland and Wildlife Management
  • Antibiotic Resistance in Bacteria
  • Veterinary medicine and infectious diseases
  • Plant pathogens and resistance mechanisms
  • Pharmaceutical and Antibiotic Environmental Impacts
  • Environmental Toxicology and Ecotoxicology
  • Per- and polyfluoroalkyl substances research
  • Antimicrobial Resistance in Staphylococcus
  • Coccidia and coccidiosis research
  • Toxin Mechanisms and Immunotoxins
  • Seed Germination and Physiology
  • Plant Stress Responses and Tolerance
  • Microbial infections and disease research
  • Plant Micronutrient Interactions and Effects
  • Synthesis and Biological Evaluation
  • Mycobacterium research and diagnosis
  • Analytical chemistry methods development
  • Legume Nitrogen Fixing Symbiosis
  • Plant responses to elevated CO2
  • Insect and Pesticide Research
  • Polyamine Metabolism and Applications
  • Escherichia coli research studies
  • Autophagy in Disease and Therapy

South China Agricultural University
2009-2025

Sanya University
2025

Northwest A&F University
2019-2024

Yangzhou University
2024

Chinese Academy of Fishery Sciences
2017-2021

Ministry of Agriculture and Rural Affairs
2019-2021

Key Laboratory of Guangdong Province
2021

Agriculture and Forestry University
2019-2020

Ministère de l'Agriculture et de la Souveraineté alimentaire
2017

Ministry of Agriculture and Agro Based Industry
2017

We developed a significantly improved ultra-high performance liquid chromatography-tandem mass spectrometry method for determination of 5-nitro-2-furaldehyde (NF) as surrogate using novel internal standard the detection nitrofurazone. used 2,4-dinitrophenylhydrazine derivatization and furfural standard. Derivatization was easily performed in HCl ultrasonic manipulation 5 min followed by extraction ethyl acetate. The samples were concentrated purified reverse phase alumina cartridges tandem....

10.1038/s41598-019-55809-0 article EN cc-by Scientific Reports 2019-12-17

The application of nanotechnology in agriculture can remarkably improve the cultivation and growth crop plants. Many studies showed that nanoparticles (NPs) made plants grow more vigorously. Light make NPs aggregated, leading to reduction toxicity. In addition, treatment with had a "hormesis effect" on this study, light-induced silver (AgNPs) were synthesized by using alfalfa (Medicago sativa L.) extracts, then optimal synthetic condition was determined. Light-induced AgNPs spherical pink,...

10.1186/s12870-022-03692-9 article EN cc-by BMC Plant Biology 2022-07-05

A credible method utilizing equilibrium dialysis and high-performance liquid chromatography with an evaporative light scattering detector (HPLC-ELSD) was developed to determine the plasma protein binding of lefamulin in swine. The demonstrated excellent linearity, a logarithmic calibration curve range 0.1 2 μg/mL (r > 0.999). Extraction recoveries from samples ranged 81.0% 90.8%, coefficients variation less than 7.21%. limits detection quantitation were established at 0.05 μg/mL,...

10.1002/bmc.70052 article EN Biomedical Chromatography 2025-03-10

In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals Guangdong province, China. The resistance-determining regions (QRDRs) gyrA and parC genes were analysed for mutations 55 harbouring only oqxAB all other PMQR genes. Overall, 282 (57.0 %) E. had at least one gene. detected 215 predominated genes, followed by qnrS (63 isolates), aac(6')-Ib-cr (56 qnrB (39 isolates) qepA (18...

10.1099/jmm.0.043307-0 article EN Journal of Medical Microbiology 2012-08-10

Lincomycin is commonly used on swine farms for growth promotion as well disease treatment and control. Consequently, lincomycin may accumulate in the environment adjacent to many ways, thereby influencing antibiotic resistance environment. Levels of lincomycin-resistance genes residues water soil samples collected from multiple sites near wastewater discharge areas were investigated this study. Sixteen 16S rRNA detected using real-time PCR. Three genes, lnu(F), erm(A), erm(B), all except...

10.3389/fmicb.2013.00364 article EN cc-by Frontiers in Microbiology 2013-01-01

The shade represents one of the major environmental limitations for turfgrass growth. Shade influences plant growth and alters metabolism, yet little is known about how affects structure rhizosphere soil microbial communities role microorganisms in responses. In this study, a glasshouse experiment was conducted to examine impact on photosynthetic capacity two contrasting shade-tolerant turfgrasses, dwarf lilyturf (Ophiopogon japonicus, OJ) shade-intolerant perennial turf-type ryegrass...

10.1186/s12870-020-2300-2 article EN cc-by BMC Plant Biology 2020-03-02

Abstract Antimicrobial resistance (AMR) is a major threat to global public health, and antibiotic genes (ARGs) are widely distributed across humans, animals, environment. Farming environments emerging as key research area for ARGs resistant bacteria (ARB). While the skin an important reservoir of ARB, transmission mechanisms between farming human remain unclear. Previous studies confirmed that swine farm environmental exposures alter microbiome, but timeline these changes ill defined. To...

10.1002/imt2.158 article EN cc-by iMeta 2024-01-01

Objectives: Multidrug-resistant (MDR) Gram-negative bacterial infections have limited treatment options due to the impermeability of outer membrane. New therapeutic strategies or agents are urgently needed, and combination therapies using existing antibiotics a potentially effective means treat these infections. In this study, we examined whether phentolamine can enhance antibacterial activity macrolide against bacteria investigated its mechanism action. Methods: Synergistic effects between...

10.3390/antibiotics12040760 article EN cc-by Antibiotics 2023-04-14

Soil salinization is a common environmental problem that seriously threatens crop yield and food security, especially through its impact on seed germination. Nanoparticle priming, an emerging treatment method, receiving increasing attention in improving stress resistance. This study used alfalfa seeds as materials to explore the potential benefits of cerium oxide nanoparticle (CeO 2 NP) priming promote germination improve salt tolerance. CeO NPs at concentrations up 500 mg/L were able...

10.3389/fpls.2023.1264698 article EN cc-by Frontiers in Plant Science 2024-01-09

Fish are vulnerable to stress from over-crowding during transportation and eugenol is the most common sedative used minimize fish injury. The ADI value of 2.5 mg/kg recommended by Joint FAO/WHO Expert Committee on Food Additives. aim this work was study elimination kinetics following exposure grass carp a bath in simulated setting. Grass carp, Ctenopharyngodon idella (120 fish) were exposed for 24 h 10 mg/L bath. Sampling performed 96 period after Eight sampled at each time point muscle,...

10.1186/s12917-017-1273-3 article EN cc-by BMC Veterinary Research 2017-11-21

Streptococcus suis isolates from diseased pigs were examined for susceptibility to nine antimicrobials, possession of virulence-associated factors (VFs), and distribution serotypes. The association between antimicrobial resistance (AMR) serotypes as well VFs was subsequently assessed. Among the investigated, serotype 2 (66.04%) mostly prevalent, followed by 1 (23.27%), 9 (1.26%), 7 (0.63%), whereas 14 untypable polymerase chain reaction typing method used. Analysis with pulsed-field gel...

10.1089/fpd.2011.1106 article EN Foodborne Pathogens and Disease 2012-06-27

A M ycoplasma gallisepticum– E scherichia coli mixed infection model was developed in broiler chickens, which applied to pharmacokinetics of valnemulin the present experiment. The velogenic . gallisepticum standard strain S6 rejuvenated establish animal model, and wild O78 injected as supplementary inoculum induce chronic respiratory disease chickens. evaluated based on its clinical signs, histopathological examination, bacteriological assay, serum plate agglutination test. infected chickens...

10.1111/jvp.12065 article EN Journal of Veterinary Pharmacology and Therapeutics 2013-06-20

The spread of antimicrobial resistance in environment is promoted at least part by the inappropriate use antibiotics animals and humans. present study was designed to investigate impact different concentrations ciprofloxacin soil containing manure on development plasmid-mediated quinolone (PMQR) - encoding genes abundance bacterial communities. For these studies, high-throughput next-generation sequencing 16S rRNA, real-time polymerase chain reaction standard microbiologic culture methods...

10.3389/fmicb.2016.01730 article EN cc-by Frontiers in Microbiology 2016-11-01

The pharmacokinetics and bioavailability of cefquinome in Beagle dogs were determined by intravenous ( IV ), intramuscular IM ) or subcutaneous SC injection at a single dose 2 mg/kg body weight BW ). minimum inhibitory concentrations MIC against 217 Escherichia coli isolated from also investigated. After injection, the plasma concentration‐time curve was analyzed using two‐compartmental model, mean values t 1/2α (h), 1/2β V ss (L/kg), Cl B (L/kg/h) AUC (μg·h/ mL 0.12, 0.98, 0.30, 0.24 8.51,...

10.1111/jvp.12225 article EN Journal of Veterinary Pharmacology and Therapeutics 2015-03-16

A practical multi-residue method based on ultra-performance liquid chromatography/tandem mass spectrometry was developed for the simultaneous determination of 23 perfluorinated alkylated substances (PFASs) in water and suspended particles. Suspended particle samples were extracted with 1% formic acid–acetonitrile cleaned by matrix solid phase dispersion extraction using a C18 sorbent graphitized carbon black. Water filtered through 0.7-μm glass fiber membranes enriched utilizing weak anion...

10.1080/10934529.2018.1528042 article EN Journal of Environmental Science and Health Part A 2018-12-06

A multi‐compartment physiologically based pharmacokinetic ( PBPK ) model to describe the disposition of cyadox CYX and its metabolite quinoxaline‐2‐carboxylic acid QCA after a single oral administration was developed in rats (200 mg/kg b.w. ). Considering interspecies differences physiology physiochemistry, efficiency validated by data set swine. The included six compartments that were blood, muscle, liver, kidney, adipose, combined compartment for rest tissues. parameterized using rat...

10.1111/jvp.12143 article EN Journal of Veterinary Pharmacology and Therapeutics 2014-11-05

The objective of this study is to investigate pharmacokinetics and dose regimens cefquinome in black swans following intravenous (IV) intramuscular (IM) administration at a single 2 mg/kg. MICs against 49 Escherichia coli isolates from were determined. Monte Carlo simulation was applied conduct the regimen assessment optimization E. swans, pharmacokinetic/pharmacodynamic (PK/PD) cutoff established for obtained study.The PK parameters T1/2α (0.31 h), T1/2β (1.69 h) ClB (0.13 L/kg·h) indicated...

10.1186/s12917-017-1148-7 article EN cc-by BMC Veterinary Research 2017-07-28
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