Le Gao

ORCID: 0000-0003-4852-8894
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About
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Research Areas
  • Plant Virus Research Studies
  • Legume Nitrogen Fixing Symbiosis
  • Soybean genetics and cultivation
  • Insect symbiosis and bacterial influences
  • Insect Resistance and Genetics
  • Plant tissue culture and regeneration
  • Plant-Microbe Interactions and Immunity
  • Transgenic Plants and Applications
  • Plant Pathogenic Bacteria Studies
  • Plant and Fungal Interactions Research
  • Animal Genetics and Reproduction
  • CRISPR and Genetic Engineering
  • Insect-Plant Interactions and Control
  • Analytical Chemistry and Chromatography
  • Seed Germination and Physiology
  • Plant Disease Resistance and Genetics
  • Cocoa and Sweet Potato Agronomy
  • Analytical chemistry methods development
  • Genetically Modified Organisms Research
  • Animal Nutrition and Physiology
  • Plant Disease Management Techniques
  • Plant pathogens and resistance mechanisms
  • Phytoplasmas and Hemiptera pathogens
  • Marine Ecology and Invasive Species
  • Magnetic and Electromagnetic Effects

Beijing Health Vocational College
2022-2025

China Medical University
2025

Nanjing Agricultural University
2014-2024

Northwest A&F University
2024

Jiaxing University
2022

Hebei University of Chinese Medicine
2019-2021

Ministry of Agriculture and Rural Affairs
2020

Rubber Research Institute
2012-2020

Chinese Academy of Tropical Agricultural Sciences
2010-2020

China Agricultural University
2019

Crop breeding during the Green Revolution resulted in high yields largely due to creation of plants with semi-dwarf architectures that could tolerate high-density planting. Although varieties have been developed rice, wheat and maize, none was reported soybean (Glycine max), few genes controlling plant architecture characterized soybean. Here, we demonstrate auxin efflux transporter PINFORMED1 (GmPIN1), which determines polar transport, regulates leaf petiole angle CRISPR-Cas9-induced...

10.1111/jipb.13269 article EN cc-by Journal of Integrative Plant Biology 2022-04-29

In this context, we reported for the first time design and development of a self-assembled nanoantiviral pesticide based on star polycation (SPc) broad-spectrum fungicide/antiviral agent seboctylamine field control Soybean mosaic virus (SMV), highly destructive plant in soybean crops. The SPc could self-assemble with through hydrogen bonds van der Waals forces, complexation reduced particle size to form spherical seboctylamine/SPc complex. addition, contact angle decreased, its retention...

10.1021/acs.jafc.4c11405 article EN Journal of Agricultural and Food Chemistry 2025-01-16

Gut microbiome influences tumorigenesis and tumor progression through regulating the microenvironment (TME) modifying blood metabolites. However, mechanisms by which gut metabolites regulate TME in multiple myeloma (MM) remain unclear. By employing16S rRNA gene sequencing coupled with metagenomics ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry, we find that Lachnospiraceae are high phosphatidylcholine (PC) low MM patients. We further show inhibits PC...

10.1038/s41467-025-57966-5 article EN cc-by-nc-nd Nature Communications 2025-03-24

Potyviral helper component-proteinase (HC-Pro) is a multifunctional protein involved in plant-virus interactions. In this study, we constructed Carica papaya L. plant cDNA library to investigate the host factors interacting with Papaya ringspot virus (PRSV) HC-Pro using Sos recruitment two-hybrid system (SRS). We confirmed that full-length calreticulin, designated PaCRT (GenBank accession no. FJ913889), interacts specifically PRSV yeast, vitro and cells SRS, protein-binding assay bimolecular...

10.1111/j.1364-3703.2009.00606.x article EN Molecular Plant Pathology 2010-01-11

Soybean mosaic virus (SMV), a potyvirus, is the most prevalent and destructive viral pathogen in soybean-planting regions of China. Moreover, other potyviruses, including bean common (BCMV) watermelon (WMV), also threaten soybean farming. The eukaryotic translation initiation factor 4E (eIF4E) plays critical role controlling resistance/susceptibility to potyviruses plants. In present study, much higher SMV-induced eIF4E1 expression levels were detected susceptible cultivar when compared with...

10.1111/mpp.12897 article EN cc-by Molecular Plant Pathology 2019-12-20

Soil salinization is a common environmental problem that seriously threatens crop yield and food security, especially through its impact on seed germination. Nanoparticle priming, an emerging treatment method, receiving increasing attention in improving stress resistance. This study used alfalfa seeds as materials to explore the potential benefits of cerium oxide nanoparticle (CeO 2 NP) priming promote germination improve salt tolerance. CeO NPs at concentrations up 500 mg/L were able...

10.3389/fpls.2023.1264698 article EN cc-by Frontiers in Plant Science 2024-01-09

Abstract Soybean mosaic virus ( SMV ) commonly affects soybean production worldwide, and the SC 18 strain has been widespread in C hina. This study aimed to characterize map resistance genes present cultivars ‘ K efeng N o. 1’ Q ihuang 22’. Inheritance analysis revealed that two independent single dominant No. 1 22 confer 18. Using simple sequence repeat SSR markers bulked segregant analysis, were located on chromosomes 2 13, respectively. We further screened populations of recombinant...

10.1111/pbr.12315 article EN Plant Breeding 2015-10-16

Comprehensive analysis of the interactions between 10 mature proteins Papaya ringspot virus type P (PRSV-P) was carried out based on a yeast two-hybrid system assay (YTHS).We detected 6 different viral (VPg-P1, VPg-P3, VPg-CI, VPg-CP, NIaPro-CI, and NIb-P3) 4 self-interactions (HC-Pro, VPg, NIaPro, CP).These did not show same directionality as corresponding in other potyviruses consequently, protein interaction matrix displayed patterns.This initial map PRSV-P allows further study various...

10.4149/av_2010_01_49 article EN Acta Virologica 2010-01-01

Soybean mosaic virus (SMV), belonging to the genus Potyvirus of family Potyviridae, has a relatively narrow host range almost exclusively confined leguminous hosts. While disease management through genetic transformation can be an effective approach, soybean remains recalcitrant routine transformation. In this context, it is important identify new hosts for SMV that used develop transgenic resistance strategies. Transformation in Nicotiana benthamiana simple and highly efficient; hence, here...

10.1094/pdis-04-14-0405-re article EN other-oa Plant Disease 2014-10-29

Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of virus, designated PLDMV-DF, infecting commercialized ringspot (PRSV)-resistant transgenic from China. Excluding 3'-poly (A) tail, shares high identity several PLDMV isolates Taiwan and Japan phylogenetically most closely related Japan. Infection PLDMV-DF in PRSV-resistant may indicate emergence this disease genetically engineered...

10.4149/av_2013_04_452 article EN Acta Virologica 2013-01-01

Soybean mosaic virus (SMV), an RNA virus, is the most common and destructive pathogenic in soybean fields. The newly developed CRISPR/Cas immune system has provided a novel strategy for improving plant resistance to viruses; hence, this study aimed engineer SMV using system. Specifically, multiple sgRNAs were designed target positive- and/or negative-sense strands of HC-Pro gene. Subsequently, corresponding CRISPR/CasRx vectors constructed transformed into soybeans. After inoculation with...

10.1016/j.cj.2024.07.007 article EN cc-by-nc-nd The Crop Journal 2024-07-31

Orthogonal experiment was applied to optimize the water extraction parameters of zinc from Flammulina velutipes, and then extracts were separated by membrane filter (0.45 μm) D101 macroporous resin. Six different species Zn obtained content various determined flame atomic absorption spectrometry. The optimized conditions for were: ratio dried material water, 1:30; temperature, 75°C; time, 120 minutes. About 34.43 μg extracted 1 g F. velutipes powder under optimal conditions. recovery value...

10.1016/j.jfda.2014.06.008 article EN cc-by-nc-nd Journal of Food and Drug Analysis 2015-01-02

Soybean is an important grain and oil crop worldwide; however, the yield seed quality of which are seriously affected by mosaic virus (SMV). As efficient detection technology crucial for field management SMV, novel immunological methods were developed in present study. According to phylogenetic analysis, CP coding sequence SMV-SC7 was selected prokaryotic expression recombinant SMV-CP. Purified SMV-CP used development polyclonal antibodies (PAb) against (PAb-SMV-CP) monoclonal (MAb)...

10.3390/ijms23169457 article EN International Journal of Molecular Sciences 2022-08-21

Soybean mosaic virus (SMV) causes significant yield losses and seed-quality deterioration in the soybean (Glycine max (L.) Merr.) growing areas of China, breeding disease-resistant cultivars is most common approach for controlling spread disease destruction crop. In this study, 97 widely grown representing nine decades (1923–2006) from four main soybean-producing subregions China (Northern Heilongjiang (NH), Mid-Southern (MSH), Jilin-Liaoning (JL) Yellow–Huai-Hai River Valleys (YHH)) were...

10.1071/cp17296 article EN Crop and Pasture Science 2018-01-01

Soybean mosaic virus (SMV) is one of the most destructive pathogens soybean (Glycine max (L.) Merr.) worldwide. In this study, 184 F7:11 recombinant inbred line (RIL) populations derived from Kefeng No. 1 × Nannong 1138-2 were used to study inheritance and linkage mapping resistance genes against SMV strains SC7 SC13 in 1. Two independent dominant (designated Rsc7 Rsc13) that control located on a molecular group (MLG) chromosome 2 (D1b). A mixed segregating population was developed by...

10.1071/cp19548 article EN Crop and Pasture Science 2020-01-01

The protein-protein interactions between viral and host proteins play an essential role in plant virus infection defense. potyviral nuclear inclusion protein a protease (NIa-Pro) is involved various steps of infection. In this study, the interacting with papaya ringspot (PRSV) NIa-Pro were screened Carica L. cDNA library using Sos recruitment two-hybrid system (SRS). We confirmed that full-length EIF3G, FBPA1, FK506BP, GTPBP, MSRB1, MTL from can interact specifically PRSV yeast, respectively....

10.4149/av_2012_01_25 article EN Acta Virologica 2012-01-01
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