Objective: To investigate the differences in odor characteristics and volatile components between raw wine‐processed Chuanxiong Rhizoma to establish a rapid differentiation method based on taste, smell, color, chemical components. Methods: Electronic tongue, electronic nose, ultraviolet spectrophotometer were used analyze 23 batches of Rhizoma. Principal component analysis, one‐way ANOVA, orthogonal partial least‐squares discriminant analysis employed differentiate alcoholic Headspace gas...

Abstract Total glucosides of paeony are the active constituents Paeoniae Radix Alba . In this study, a novel strategy was proposed to find more metabolites and differences between paeoniflorin, albiflorin total (TGP). This characterized as follows: firstly, animals were divided into three groups (paeoniflorin, TGP) identify source TGP from paeoniflorin or albiflorin; secondly, generic information‐dependent acquisition scan for low‐level triggered by multiple mass defect filter dynamic...

As a classic TCM prescription, LGZG has been widely used in clinical prevention and treatment of heart failure, nonalcoholic fatty liver, hyperlipidemia. However, there are few studies on chemical components recent years, the basis quality evaluation is not sufficient. This study was to find active ingredients Lingguizhugan decoction using UPLC-MS/MS network pharmacology. By comparing retention time MS dates reference self-building database, cleavage rules composition whose mass errors less...

Abstract Zhi‐zi‐chi decoction (ZZCD) is a classical formula widely used in Chinese clinical application. In the present study, novel and efficient strategy has been developed for screening identification of multiple constituents their metabolites ZZCD using ultra‐high‐performance liquid chromatography combined with triple time‐of‐flight mass spectrometry. The approach an online data acquisition method dependent on defect filter dynamic background subtraction processing techniques. First,...

Cyperus rotundus (CR) is the dry rhizome of Cyperaceae plant Cyperus, which widely used in food processing, such as curry, pickling spices, and baking goods, well medicinally after stir-frying. The level stir-frying (slightly stir-frying, yellow, black) has a direct impact on flavor, aroma, pharmacological effects food. However, distinctions between various levels have not been studied. purpose this study was to develop method that combines electronic tongue, nose, HS-GC-MS elucidate flavor...

ABSTRACT Sanhua Decoction (SHD) is a classic prescription for the treatment of stroke in clinic. Based on combination strategy vitro and vivo, chemical constituents SHD were characterized by ultra‐high‐performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry, metabolites five effective anthraquinone aglycones (emodin, chrysophanol, rhein, aloe‐emodin, physcion) rhubarb studied. A total 222 compounds isolated identified vitro, including 50 flavonoids...

Total glucosides of paeony (TGP) have been confirmed to be hepatoprotective. However, the underlying mechanism is largely unclear. In this study, we investigated metabolic profiles urine and serum in rats with carbon tetrachloride- (CCl 4 -) induced experimental liver injury TGP administration by using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). The vehicle or a single dose was intragastrically administered Wistar once day for 14 consecutive days. To induce ALI, 50%...

Schisantherin A is an active ingredient originating from Schisandra chinensis (Turcz.) which has hepatoprotective and anti-oxidation activities. In this study, in vitro metabolisms investigated on rat liver microsomes (RLMs) vivo explored male Sprague Dawley rats of were tested, respectively. The metabolites identified using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Based the method, 60...

Molecularly imprinted polymers (MIPs) have been attracting increasing attention and used in various applications.

Yangwei decoction, a classical traditional Chinese medicine prescription, has been widely used to treat exogenous cold and internal injury with damp stagnation for many centuries. However, its systematic chemical profiling remains ambiguous, which hampered the interpretation of pharmacology mechanism formula. In present study, ultra-performance liquid chromatography coupled electrospray ionization quadrupole time-of-flight tandem mass spectrometry method was successfully established first...

Shaoyao Gancao Decoction (SGD) is a classic prescription of traditional Chinese medicine (TCM), which composed Paeoniae Radix Alba and Glycyrrhizae et Rhizoma, has the clinical effect anti-liver injury, but its active ingredients are unclear. In this study, joint application phytochemical compositional analysis, network pharmacology, molecular docking technology was utilized to screen components SGD against liver injury. Firstly, total 110 compounds were identified by UPLC-Q-TOF-MS/MS,...

This present study aims to establish a UPLC method for simultaneously determining eleven components such as new chlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,artichoke,isochlorogenic acid A,isochlorogenic B,isochlorogenic C,rutin,hibisin and loganin in Lonicerae Japonicae Flos,Lonicerae Caulis leaves of Lonicera japonica comparing the differences contents phenolic acids,flavonoids iridoid glycosides japonica.The was carried out on an ACQUITY BEH C18column(2.1 mm×100...

An imidazolium-type ionic liquid and polyhedral oligomeric silsesquioxane were combined to produce an imprinted monolith in capillary endowed with wide selectivity enrich baicalin its analogues for analysis by multidimensional LC systems.

Chemical research of the medicinal plant Hemsleya amabilis (Cucurbitaceae) yielded five new cucurbitane-type triterpenes hemslelis A⁻E (1⁻5) by silica gel column, ODS and semi-HPLC techniques. Their structures were determined spectroscopic analysis examined alongside existing data from prior studies. Compounds 1⁻5 evaluated for their cytotoxic activities against three human tumor cell lines, Hela, HCT-8, HepG-2, with IC50 ranging 5.9 to 33.9 μM compared Cisplatin.

Cyanotis arachnoidea C. B. Clarke is a traditional Chinese medicinal herb that has limited clinical use in the treatment of diabetes mellitus (DM) minority areas Guizhou China. However, few prior reports are available on quality control , and its markers hypoglycemic mechanism still unclear. The purpose this study to explore (Q-markers) predict mechanism. In study, ultra-high-performance liquid chromatography (UHPLC) fingerprint combined with chemical pattern recognition were performed, four...

The survival of Lactobacillus plantarum suspended in 100 m L physiological saline and vaccinated g pickled lettuce was investigated microwave power 600 W,after 0,30,60,90,120 seconds,got the dose sterilization. Then effects sterilization pasteurized on quality properties such as texture,color sensory evaluation explored,in order to obtain a better process for lettuce.The results showed that mid- bottom part suitable eliminate error caused by uniformity. plantarum( density was3.4 × 108 CFU /...

To establish the method for determining non-volatile ingredients of neochlorogenic acid, chlorogenic cryptochlorogenic caffeic isochlorogenic acid A, rosmarinic ferulic rutin, luteoloside, isoquercitrin, hesperidin, diosmin, diosmetin, luteolin, acacetin and linarin in Menthae Haplocalycis Herba formula granules traditional herbal pieces by UPLC-MS/MS, analyze correlation pieces. Shim-pack GIST C_(18) column(2.1 mm×100 mm, 2 μm) was adopted with acetonitrile-0.1% formic aqueous solution as...

Abstract Hydrophilic interaction liquid chromatography (HILIC), as a powerful LC method for polar compounds, has gained increasing attention in developing new HILIC stationary phases and analytical methods. Instead of using standardized substance representing different molecular force types to evaluate chromatographic performance, the analyte targets, structural analogues matrine oxymatrine, are applied modulate preparation carboxyl group modified column mode. The feasibility...