ABSTRACT Oleanolic acid (OA), a pentacyclic triterpenoid, exhibits potential anti‐tumor activity against many tumor cell lines. This study aims to examine the of OA on pancreatic cancer cells and its molecular mechanism. The results showed that proliferation Panc‐28 was inhibited by in concentration‐dependent manner, with an IC 50 (The half maximal inhibitory concentration) value 46.35 µg ml −1 , as determined MTT (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay. cycle...

Predictive value of serum interleukin 10 (IL-10) and 18 (IL-18) expression in patients with colorectal cancer (CRC) was investigated. This study retrospectively analyzed the medical records 146 CRC admitted to Binzhou Medical University Hospital as group, collected physical examination data 82 volunteers control group. The levels IL-10 IL-18 were measured by enzyme-linked immunosorbent assay (ELISA). A 60-month follow-up on group performed make their prognosis. expressions statistically...

Introduction: This study aimed to assess the alleviative effect of quercetagetin (QG) on zearalenone (ZEN)-induced liver injury in rabbits. Methods: Ninety 41-day-old healthy Hyla rabbits were randomly assigned into three groups, including a control (fed with basic diet), ZEN addition group diet + 600 μg/kg ZEN), and QG 100 mg/kg QG), 30 per group. The duration experiment was 28 days. Results: results revealed no significant differences average daily gain, feed intake, gain ratio liver,...

Zearalenone (ZEA), a prevalent mycotoxin in animal feeds, is known to disrupt normal ovarian development and function due its estrogenic activity. This study investigates the toxic effects of ZEA on ovaries meat rabbits explores underlying mechanisms. Ninety healthy 41-day-old Hyla male were randomly assigned into three groups. The control group received basal diet, while experimental groups fed diets supplemented with 300 600 μg/kg ZEA, respectively. Each consisted 30 replicates, one rabbit...

The purpose of this study was to design novel drug nanocrystals (NCs) stabilized by glycyrrhizic acid (GL) for achieving liver targeted delivery due the presence GL receptor in hepatocytes. Quercetin (QT) exhibits good pharmacological activities treatment diseases, including steatosis, fatty hepatitis, fibrosis, and cancer. It selected as a model owing its poor water solubility. QT NCs (QT-NCs/GL) were fabricated wet media milling technique systemically evaluated. QT-NCs poloxamer 188...

To investigate the neurotoxicity of colistin, female mice received colistin sulfate (7.5 mg/kg/12 h) intravenously for 7 days successively, behavioral changes, and neuropathological electrophysiological characterizations sciatic nerves were determined prior to administration at 1, 3, 15 thereafter. At days, compound action potential durations (CAPDs), muscle amplitudes (CAPAs), conduction velocities sciatic-tibial nerve (NCVs) showed progressively abnormal changes with time prolonged....

The present study investigated the expression of excision repair cross-complementing gene 1 (ERCC1) and thymidylate synthase (TYMS) in patients with colorectal cancer predictive value chemotherapy. Eighty chemotherapy admitted to Binzhou Medical University Hospital from June 2013 2015 were randomly selected, 80 tissues 68 adjacent taken for analysis. RT-qPCR was used detect ERCC1 as well level TYMS. relationship efficacy, clinical pathology survival time receiving standard chemotherapy,...

As a classic TCM prescription, LGZG has been widely used in clinical prevention and treatment of heart failure, nonalcoholic fatty liver, hyperlipidemia. However, there are few studies on chemical components recent years, the basis quality evaluation is not sufficient. This study was to find active ingredients Lingguizhugan decoction using UPLC-MS/MS network pharmacology. By comparing retention time MS dates reference self-building database, cleavage rules composition whose mass errors less...

Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q High Performance (HP) anion Butyl HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited potent activity against the human lung cancer A549 cell line (IC 50 , 18.76...

Danggui Jianzhong decoction is a classical prescription that has been widely used for thousands of years. However, the quality this formula difficult to control owing its complex chemical component system. In study, simple and efficient method comprising ultra-high-performance liquid chromatography fingerprint, pattern recognition, network pharmacology was established evaluate decoction. A total 20 common peaks were obtained by fingerprint analysis 19 chemicals identified. The similarity 15...

Five phospholipid classes of <italic>Penaeus vannamei </italic>head were separated, analyzed and quantified. They had different cardiovascular activities evaluated in zebrafish models, which may provide a research basis for pharmaceutical use marine phospholipids.

In plant cells, multiple paralogs from ribosomal protein (RP) families are always synchronously expressed, which is likely contributing to ribosome heterogeneity or functional specialization. However, previous studies have shown that most RP mutants share common phenotypes. Consequently, it difficult distinguish whether the phenotypes of resulted loss specific genes a global deficiency. Here, investigate role gene, we employed gene overexpression strategy. We found Arabidopsis lines...

Grapevine (Vitis vinifera) is an important and popular perennial fruit tree cultivated worldwide. ripening affected by flowering time, although members of the MADS-box protein family play vital roles in regulating plants, functions proteins grapevine remain largely unknown. AGAMOUS-LIKE 11 (VvAGL11), a gene grapevine, was reported to be regulator seed morphogenesis. In this study, heterologous overexpression VvAGL11 found significantly promote Arabidopsis, suggesting that its active...

Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) involved 60S ribosome yeast, but its role Arabidopsis (A. thaliana) remains exclusive. Here, we found that NLE (AtNLE) highly conservative phylogeny, which encoding WD40-repeat protein. AtNLE expressed actively dividing tissues. AtNLE-GFP localized the nucleus. physically interacts with MIDAS domain of AtMDN1, protein ribosomal subunit Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced...

ABSTRACT Cells of gastric cancer invade local tissue extensively and also metastasize through the circulation to remote organs. Patients with metastasized have poor outcomes. Cancer cells are known release extracellular vesicles (EVs) that contribute progression, but how cell-derived EVs promote growth metastasis remains poorly understood. We recently reported levels circulating (gcEVs) adhesive ligand von Willebrand factor (VWF) associated prognosis patients, underlying mechanism this...

Cyanotis arachnoidea C. B. Clarke is a traditional Chinese medicinal herb that has limited clinical use in the treatment of diabetes mellitus (DM) minority areas Guizhou China. However, few prior reports are available on quality control , and its markers hypoglycemic mechanism still unclear. The purpose this study to explore (Q-markers) predict mechanism. In study, ultra-high-performance liquid chromatography (UHPLC) fingerprint combined with chemical pattern recognition were performed, four...

ObjectiveTo explore the distribution of genetic polymorphisms (rs2834497 and rs4817656) beta-subunit potassium channel gene (KCNE1) in healthy Chinese Hart Uighur population Xinjiang. Methods 409 406 Han persons were studied for genotypes KCNE 1 by allele-specific PCR (AS-PCR). Results The frequencies AA, AG GG rs2834497 65.5％ ,29.8％ ,and 4.6％ group while ones 55.4％ ,36.9 ％ 7.6 group. There was significant difference between these two groups (x2 = 9.92, P ＜ 0.01 ). CC, CT TT rs4817656 26.4...

Triple‑negative breast cancer (TNBC) is highly invasive, has a high rate of recurrence and associated with poor clinical outcome when compared non‑TNBC due to lack effective targeted treatments. The coatomer protein complex subunit β2 (COPB2) upregulated in various types malignant cancer. present study demonstrated that COPB2 expression levels were significantly carcinoma HS‑578T cells (clonal originating from TNBC) MCF‑7 cells. also exhibited higher rates proliferation, invasion...