A5077175091- Fungal and yeast genetics research
- Genetic Neurodegenerative Diseases
- Postharvest Quality and Shelf Life Management
- Phytochemicals and Antioxidant Activities
- Plant Physiology and Cultivation Studies
- Antifungal resistance and susceptibility
- Antibiotic Resistance in Bacteria
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Insect and Pesticide Research
- Plant Gene Expression Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Insect-Plant Interactions and Control
- Plant nutrient uptake and metabolism
- Developmental Biology and Gene Regulation
- Fungal Infections and Studies
- Liver Disease Diagnosis and Treatment
- Plant Virus Research Studies
- Advanced Fluorescence Microscopy Techniques
- Insect Pest Control Strategies
- Retinal Development and Disorders
- Salmonella and Campylobacter epidemiology
- Bacterial biofilms and quorum sensing
- Ferroptosis and cancer prognosis
- Coconut Research and Applications
University of Illinois Chicago
2021-2025
Shanxi Agricultural University
2025
Zhejiang A & F University
2025
Zhejiang Chinese Medical University
2024
Ministry of Agriculture and Rural Affairs
2024
Sichuan Agricultural University
2024
Western University
2016-2023
McMaster University
2023
South China Agricultural University
2020-2022
Hainan University
2022
Dear editor, Making precise changes in the genomes of organisms is challenging for most genome editing tools. Recently, a search-and-replace method, also known as prime editing, was developed that can introduce user-defined sequence into target site without requiring double-stranded breaks (DSBs) or repair templates (Anzalone et al., 2019). The editor contains Moloney murine leukaemia virus reverse transcriptase (M-MLV RT) fused to C terminus SpCas9 (H840A) nickase This fusion protein guided...
Abstract Heterochromatin is a tightly packed form of chromatin that associated with DNA methylation and histone 3 lysine 9 (H3K9me). Here, we identify an H3K9me2-binding protein, Agenet domain (AGD)-containing p1 (AGDP1), in Arabidopsis thaliana . Here find AGDP1 can specifically recognize the H3K9me2 mark by its three pairs tandem AGDs. We determine crystal structure 1 2 cassette (AGD12) Raphanus sativus complex peptide. In complex, peptide adopts unique helical conformation. AGD12...
To investigate the prevalence and transmission of mcr-3 among Salmonella enterica serotype Typhimurium 1,4,[5],12:i:-.A total 4724 clinical isolates were screened for presence in China during 2014-19. The clonal relationship mcr-3-positive their plasmid contents complete sequence also characterized based on WGS data from Illumina MinION platforms.We identified 10 isolates, all MDR, mostly resistant to colistin, cefotaxime, ciprofloxacin, doxycycline florfenicol. was co-present with...
Hepatic lipotoxicity, resulting from excessive lipid accumulation in hepatocytes, plays a central role the pathogenesis of various metabolic liver diseases. Despite recent progress, precise mechanisms remain incompletely understood. Employing exposure to palmitate hepatocytes as our primary experimental model and mice studies, we aimed uncover behind hepatic thereby developing potential treatments. Our data reveal for first time that leads downregulated expression poly(ADP-ribose) polymerase...
Changes in the number of mold spores and color difference values cardboard were evaluated during molding process paper wine boxes. The experiment utilized three types cardboard: single white industrial paperboard (Q), grey-offset (S), grey-coated (T), along with nine strains collected from mold-contaminated box samples. molds identified using both morphological molecular techniques. These inoculated on surface incubated at 28 °C 98% relative humidity for days to assess values. results...
Organ development is directed by selector gene networks. Eye in the fruit fly Drosophila melanogaster driven highly conserved network referred to as "retinal determination network," composed of approximately 20 factors, whose core comprises twin eyeless (toy), (ey), sine oculis (so), dachshund (dac), and eyes absent (eya). These genes encode transcriptional regulators that are each necessary for normal eye development, sufficient direct ectopic when misexpressed. While it well documented...
Non-alcoholic fatty liver disease (NAFLD) is a challenging to interfere with and represents potential long-term risk factor for hepatic fibrosis cancer. The Xiezhuo Tiaozhi (XZTZ) formula, water extract from crude herbs, has been widely used as an anti-NAFLD agent through clinical observation. However, the underlying pharmacological mechanisms of XZTZ formula its impact on pathways against NAFLD have not elucidated. Our study aims investigate effects regulatory treat NAFLD. possible active...
SummaryThe browning of litchi fruit pericarp caused by water loss was investigated in relation to total phenolic concentration, phenylalanine ammonia-lyase (PAL) activity, reduced glutathione content, polyphenol oxidase (PPO) and peroxidase (POD) activities, peroxide content. Development increased with storage relative humidity (r.h.; from 90% 60% r.h.) time (from 1–3 d), there a strong 2 r.h. interaction. Water the PAL activity stored at or 70% maximum after d storage, while this enzyme...
To investigate the prevalence and molecular characteristics of fosA3 fosA7 among Salmonella isolates.Five hundred fifty-one isolates collected from food animals in China during 2016-19 were screened for fos genes. The drug resistance, serovars, clonal relationships genetic environments fosA compared between fosA7- fosA3-positive Salmonella.A relatively high (9.26%) (6.53%) was identified. associated with high-level fosfomycin resistance (≥512 mg/L), while conferred low-level that independent...
The palette of fluorescent proteins (FPs) available for live-cell imaging contains that strongly differ in their biophysical properties. FPs cannot be assumed to equivalent and certain cases could significantly perturb the behavior reporters. We employed Saccharomyces cerevisiae comprehensively study impact on toxicity polyglutamine (polyQ) expansion associated with Huntington's disease. polyQ fusion constructs is highly dependent sequences flanking repeats. Thus, they represent a powerful...
Abstract Active DNA demethylation is critical for altering methylation patterns and regulating gene expression. The 5‐methylcytosine glycosylase/lyase ROS1 initiates a base‐excision repair pathway active required the prevention of hypermethylation at 1 000s genomic regions in Arabidopsis . How regulated targeted to specific not well understood. Here, we report discovery an protein complex that contains ROS1, regulates expression, likely targets regions. physically interacts with WD40 domain...
We investigated the prevalence and transmission of NDM-producing Enterobacteriaceae in fecal samples geese environmental from a goose farm southern China. The were cultivated on MacConkey agar plates supplemented with meropenem. Individual colonies examined for blaNDM, blaNDM-positive bacteria characterized based whole-genome sequencing (WGS) data Illumina Oxford Nanopore Technologies (ONT) platforms. Of 117 analyzed, carriage rates New Delhi metallo-β-lactamase (NDM)-positive 47.1, 18, 50%...
Surface browning after harvest is the primary constraint affecting storage life and market circulation of rambutans. In this study, rambutan fruits were soaked in sodium nitroprusside at different concentrations stored 25°C for 8 days to explore effects on postharvest quality browning. The weight loss, index superoxide anion radical, hydrogen peroxide malondialdehyde contents treated reduced compared those control (soaked distilled water). And with had a higher total phenolic content lower...
Nonalcoholic fatty liver disease (NAFLD) causes significant global mortality and healthcare costs with no recommended pharmacological intervention for clinical management. Nuciferine (Nuc) is an alkaloid aromatic rings, abundantly found in
Abstract The spotted alfalfa aphid, Therioaphis trifolii maculata (Buckton), caused local browning of cells surrounding feeding sites on lucerne plants (cv. Hunter River). Aqueous extracts infested leaves underwent a marked process that did not occur in healthy leaves. was accelerated by addition tyrosinase and peroxidase reversed reducing agents such as ascorbate glutathione. In the presence added agents, produced brown precipitates, probably conjugates phenolics with leaf proteins similar...
Defined as the dysfunction and/or cell death caused by toxic lipids accumulation in hepatocytes, hepatic lipotoxicity plays a pathological role nonalcoholic fatty liver disease. The cellular and molecular mechanisms underlying remain to be elucidated. In this study, using AML12 cells, nontransformed murine hepatocyte line, exposed palmitate (a 16-C saturated acid) an experimental model, we investigated of nicotinamide N-methyltransferase (NNMT), methyltransferase catalyzing methylation...
Chromatin remodeling regulates gene expression in response to the accumulation of misfolded polyQ proteins associated with Huntington's disease (HD). Tra1 is an essential component both SAGA/SLIK and NuA4 transcription co‐activator complexes linked multiple cellular processes, including protein trafficking signaling pathways stress. Cells compromised activity display phenotypes distinct from deletions encoding components SAGA complexes, indicating a potentially unique regulatory role...
Abstract Candida albicans is the most common cause of death from fungal infections. The emergence resistant strains reducing efficacy first-line therapy with echinocandins, such as caspofungin calls for identification alternative therapeutic strategies. Tra1 an essential component SAGA and NuA4 transcriptional co-activator complexes. As a PIKK family member, characterized by C-terminal phosphoinositide 3-kinase domain. In Saccharomyces cerevisiae, assembly function are compromised variant...