A5101552377- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Evolution and Genetic Dynamics
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Plant Molecular Biology Research
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Parasitic Infections and Diagnostics
- Plant Stress Responses and Tolerance
- Research in Cotton Cultivation
- Phytase and its Applications
- Generative Adversarial Networks and Image Synthesis
- COVID-19 impact on air quality
- Impact of Light on Environment and Health
- Plant Gene Expression Analysis
- Iron Metabolism and Disorders
- Oral health in cancer treatment
- Plant Virus Research Studies
Second Affiliated Hospital of Zhejiang University
2022-2025
Ministry of Agriculture and Rural Affairs
2024
Fujian Agriculture and Forestry University
2022-2024
Zhengzhou University
2024
Henan Agricultural University
2024
University of Illinois Urbana-Champaign
2023-2024
Henan University
2024
Shihezi University
2019
Central South University
2013
Third Xiangya Hospital
2013
TMexCD1-TOprJ1, which is associated with phenotypic resistance to multiple classes of antibiotics, a transmissible resistance-nodulation-division (RND) family efflux pump. However, the prevalence and genomic characteristics clinical isolates this important determinant are poorly understood. In study, we aimed survey tmexCD-toprJ among Gram-negative collected from hospitals in China between 1991 2020 characterise tmexCD-toprJ-positive isolates.We conducted online data retrieval active...
Acinetobacter baumannii is one of the key Gram-negative pathogens that can cause serious nosocomial infections. In China, a large proportion clinical A. strains are multidrug resistant, among which resistant to carbapenems particularly worrisome, as infections caused by such may limit choice existing antibiotics. We conducted nationwide and genome-based surveillance on prevalence antibiotic susceptibility profile carbapenem-resistant (CRAB) collected from intensive care units (ICUs) in...
Abstract Background Carbapenem-resistant Escherichia coli (CREC) has been considered as WHO priority pathogens, causing a great public health concern globally. While CREC from patients thoroughly investigated, the prevalence and underlying risks of in healthy populations have overlooked. Systematic research on individuals was conducted here. We aimed to characterize collected China between 2020 2022 compare genomes isolates isolated clinical patients. Methods present nationwide investigation...
Considerable attention is given to intensive care unit-acquired infections; however, research on the transmission dynamics of multichain carbapenemase-resistant Enterobacter cloacae complex (CRECC) outbreaks remains elusive. A total 118 non-duplicated CRECC strains were isolated from clinical, intestinal, and hospital sewage samples collected Zhejiang province China during 2022–2023. 64 samples, their prevalence increased 10.0 % (95 confidence interval, CI = 0.52–45.8 %) in 2022 63.6...
The global transmission of carbapenem-resistant Acinetobacter baumannii (CRAB) poses a significant and grave threat to human health. To investigate the potential relationship between hospital sewage CRAB within healthcare facilities, isolates spp. obtained from untreated samples were subjected antimicrobial susceptibility tests, genome sequencing, bioinformatic phylogenetic tree analysis, that data matched with those clinical isolates. Among 70 tested, A. was most prevalent detectable in 5...
Carbapenem-resistant Enterobacterales (CRE) are increasingly recognized as an urgent public health concern. The rapid and accurate identification of carbapenemases could provide insights into antimicrobial therapy infection control. In this study, we evaluated the efficacy three different methods, including NG-test Carba 5, colloidal gold immunoassay (CGI) test, Xpert Carba-R assay, for detection five (KPC, NDM, IMP, OXA-48, VIM). A total 207 Gram-negative strains collected from patients...
Purpose Carbapenem-resistant Klebsiella pneumoniae (CRKP) producing carbapenemases poses a global threat to public health. Antimicrobial agent combinations have been promoted as potential therapeutic strategy for infections. The most effective antimicrobial against CRKP strains different are currently unclear, particularly those the KPC variant carbapenemases. This study is aimed evaluate effectiveness of various with Methods A checkerboard assay involving 24 was conducted on 44...
The ionic toxicity induced by salinization has adverse effects on the growth and development of crops. However, researches salt tolerance in plants have focused primarily cations such as sodium ions (Na+), with very limited studies chloride (Cl−). Here, we cloned homologous genes Arabidopsis thaliana AtCLCc, GhCLCc-1A/D, from upland cotton (Gossypium hirsutum), which were significantly NaCl or KCl treatments. Subcellular localization showed that GhCLCc-1A/D both localized to tonoplast....
has emerged as a growing public health concern due to its ability cause various infections and increasing resistance antibiotics. To assess current epidemiology in clinical settings, we conducted survey involving 426 healthy individuals 273 ICU inpatients at provincial hospital China. Our findings revealed high prevalence of
A novel Klebsiella pneumoniae carbapenemase (KPC) variant, KPC-93, was identified in two clinical isolates from a patient China treated with ceftazidime-avibactam. KPC-93 possessed five-amino-acids insertion (Pro-Asn-Asn-Arg-Ala) between Ambler positions 267 and 268 KPC-2. Cloning expression of the blaKPC-93 gene Escherichia coli, followed by determination minimum inhibitory concentration (MIC) values kinetic parameters, showed that exhibited increased resistance to ceftazidime-avibactam,...
A rectal swab sample was collected from a patient with Guillain-Barré syndrome and enriched in lysogeny broth. Carbapenem-resistant bacteria were selected by China Blue agar plates containing 0.3 μg/ml meropenem. Carbapenemase-producing Klebsiella michiganensis identified characterized matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), immune colloidal gold technique, conjugation experiment, PCR analysis, antimicrobial susceptibility testing. The genome of K. determined...
Ceftazidime-avibactam (CAV) is a new treatment option against carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. However, the rapid emergence of CAV resistance mediated by KPC variants has posed severe threat to healthcare after its clinical application. The characteristics in CRKP strains needs be determined China. A total 477 isolates were collected from 46 hospitals Zhejiang Province 2018 2021. results demonstrated that had potent activity 94.5% all (451/477, 95% CI:...
Abstract The human intestinal tract is considered the most important reservoir of opportunistic pathogens, including Pseudomonas aeruginosa, which often overlooked but critical due to its antimicrobial resistance and virulence. Public health interventions control this pathogen require a comprehensive understanding epidemiology genomics. In current study, we identified P. aeruginosa strains from 2,605 fecal samples collected between 2021 2022. Among these samples, 574 were ICU inpatients in...
The phytase from Mitsuokella jalaludinii, a novel phytase-producing rumen bacterium, was purified 120-fold to near homogeneity and characterized. completely cell-associated about half of the enzyme activity released when bacterial cells were incubated with 1.5 mol/l KCl solution for 8 h. optimum pH in range 4.0 5.0 temperature 55 60°C. stable at 7.0. It highly specific sodium phytate as substrate, strongly inhibited by Cu2+, Zn2+, Fe2+and Fe3+, significantly stimulated Ba2+ slightly Mn2+...
The objective of this study is to determine the prevalence antibiotic resistance factors, including production extended-spectrum beta-lactamases (ESBLs) and presence class 1 integrons among Escherichia coli isolated from clinical specimens.Bacterial species identification was performed using a VITEK-2 system (VITEK2 GN-card; bioMérieux, France). Antimicrobial susceptibility testing determined disk diffusion method according 2010 Clinical Laboratory Standards Institute guidelines. Polymerase...
CG258 is the dominant carbapenemase-producing Klebsiella pneumoniae clone worldwide and treatment of infections caused by this relies largely on last-line antibiotics, colistin, tigecycline. However, emergence global dissemination mcr tmexCD1-toprJ1 genes have significantly compromised their clinical applications. K. carrying both not been reported. A colistin-resistant strain T698-1 belonging to ST1326, a member CG258, was isolated from intestinal sample patient characterized antimicrobial...
Over the past few decades, antimicrobial resistance (AMR) has emerged as a global health challenge in human and veterinary medicine. Research on AMR genes captive wild animals increased. However, presence molecular characteristics of tet(X)-carrying bacteria these remain unknown. Eighty-four samples were collected from animals. tet(X) variants detected using polymerase chain reaction isolates identified matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. All...