A5100657061- Kruppel-like factors research
- Angiogenesis and VEGF in Cancer
- Plant Reproductive Biology
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- Autism Spectrum Disorder Research
- Peroxisome Proliferator-Activated Receptors
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Immune Response and Inflammation
- Cancer-related gene regulation
- Animal Genetics and Reproduction
- Medicinal Plants and Bioactive Compounds
- Sperm and Testicular Function
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Atherosclerosis and Cardiovascular Diseases
- DNA Repair Mechanisms
- Research in Cotton Cultivation
- Lipid metabolism and disorders
First Affiliated Hospital of Jiangxi Medical College
2025
Nanchang University
2025
Brigham and Women's Hospital
2004-2024
Cotton Research Institute
2024
Chinese Academy of Agricultural Sciences
2024
Harvard University
2000-2024
Zhejiang University
2019-2024
Nanjing Institute of Vegetable Science
2019-2024
Henan Agricultural University
2024
Institute of Zoology
2020-2023
In the face of systemic risk factors, certain regions arterial vasculature remain relatively resistant to development atherosclerotic lesions. The biomechanically distinct environments in these geometries exert a protective influence via key functions endothelial lining; however, mechanisms underlying coordinated regulation specific mechano-activated transcriptional programs leading functional phenotypes have remained elusive. Here, we show that transcription factor Kruppel-like 2 (KLF2) is...
Atherosclerotic lesion localization to regions of disturbed flow within certain arterial geometries, in humans and experimental animals, suggests an important role for local hemodynamic forces atherogenesis. To explore how endothelial cells (EC) acquire functional/dysfunctional phenotypes response vascular region-specific patterns, we have used vitro dynamic system accurately reproduce shear stress waveforms on cultured human EC examined the effects gene expression by using a high-throughput...
Upregulated by atheroprotective flow, the transcription factor Krüppel-like 2 (KLF2) is crucial for maintaining endothelial function. MicroRNAs (miRNAs) are noncoding small RNAs that regulate gene expression at posttranscriptional level. We examined role of miRNAs, particularly miR-92a, in flow-regulated KLF2.Dicer knockdown increased level KLF2 mRNA human umbilical vein cells, suggesting regulated miRNA. In silico analysis predicted miR-92a could bind to 3' untranslated region mRNA....
Local patterns of biomechanical forces experienced by endothelial cells (ECs) in different vascular geometries appear to play an essential role regulating EC function and determining the regional susceptibility atherosclerosis, even face systemic risk factors. To study how regulate redox homeostasis, important pathogenic factor atherogenesis, we have cultured human ECs under 2 prototypic arterial shear stress waveforms, “atheroprone” “atheroprotective,” which were derived from distinct...
Vascular endothelium is a dynamic cellular interface that displays unique phenotypic plasticity. This plasticity critical for vascular function and when dysregulated pathogenic in several diseases. Human genotype-phenotype studies of are limited by the unavailability patient-specific endothelial cells. To establish platform studying biology, we have generated from human induced pluripotent stem cells (iPSCs) exhibiting rich functional mature primary endothelium. These respond to diverse...
Vascular pathologies constitute a major cause of graft rejection after organ transplantation. Recent studies have documented an improvement in transplant outcome when organs are preserved through pulsatile perfusion; however, the underlying mechanisms these observations poorly characterized. We hypothesized that temporary absence flow occurring context cold storage conditions disrupts endothelial vasoprotective programs, and this consequence stasis may be target for pharmacological...
The transcription factor Krüppel-like 2 (KLF2) is required for the quiescent and migratory properties of naive T cells. Statins, a class HMG-CoA reductase inhibitors, display pleiotropic immunomodulatory effects that are independent their lipid-lowering capacity may be beneficial as therapeutic agents cell-mediated inflammatory diseases. Statins upregulate KLF2 expression in endothelial cells, this activity associated with an antiinflammatory phenotype. We therefore hypothesized statins due,...
Male-sterile plants provide an important breeding tool for the heterosis of hybrid crops, such as Brassicaceae. In last decade, circular RNAs (circRNAs), a novel class covalently closed and single-stranded endogenous non-coding (ncRNAs), have received much attention because their functions “microRNA (miRNA) sponges” “competing RNAs” (ceRNAs). However, information about circRNAs in regulation male-sterility anther development is limited. this study, we established Polima cytoplasm male...
UVA can cause oxidative stress and photoaging of cells. We established a UVA-induced model human fibroblasts focused on the antioxidant anti-photoaging ability Lactobacillus plantarum fermented Rhodiola rosea. Compared with unfermented rosea, rosea has better DPPH free radical hydroxyl scavenging ability, significantly reduces content reactive oxygen species (ROS), improves level. Further studies have shown that activate Nrf2/Keap1 signaling pathway up-regulate heme oxygenase-1 (HO-1),...
Heterobifunctional cross-linker 1 facilitates preparing various functional agents bearing a dinitroimidazole warhead via the SPAAC reaction and does well in protein oligonucleotide functionalization.
Alterations in hemodynamic shear stress acting on the vascular endothelium are critical for adaptive arterial remodeling. The molecular mechanisms regulating this process, however, remain largely uncharacterized. Here, we sought to define responses evoked endothelial cells exposed waveforms characteristic of coronary collateral vessels and subsequent paracrine effects smooth muscle cells. A lumped parameter model human circulation was used simulate normal remodeling waveforms. These were...
Abstract Changing ambient temperature often impairs plant development and sexual reproduction, particularly pollen ontogenesis. However, mechanisms underlying cold stress-induced male sterility are not well understood. Here, we exposed Chinese cabbage (Brassica campestris) to different conditions during flowering demonstrated that the tetrad stage was most sensitive. After completion of at optimal conditions, transient stress still impacted auxin levels, starch lipid accumulation,...
Genic male sterility (GMS) line is an important approach to utilize heterosis in Brassica rapa, one of the most widely cultivated vegetable crops Northeast Asia. However, molecular genetic mechanisms GMS remain be largely unknown.Detailed phenotypic observation 'Bcajh97-01A/B', a B. rapa genic sterile AB this study revealed that aberrant meiotic cytokinesis and premature tapetal programmed cell death occurring ultimately resulted microspore degeneration pollen wall defect. Further gene...
Abstract The overwhelming majority of Rett syndrome cases are caused by mutations in the gene MECP2 . has two isoforms, termed MECP2_e1 and MECP2_e2, which differ their N‐terminal amino acid sequences. A growing body evidence indicated that may be etiologically relevant isoform Syndrome based on its expression profile brain because, strikingly, no have been discovered affect MECP2_e2 exclusively. In this study we sought to characterize four classical patients with putatively only isoform....
Abstract Background Pluripotency, the property of a cell to differentiate into all cellular types given organism, is central development stem cell-based therapies and regenerative medicine. Stem pluripotency result orchestrated activation complex transcriptional network characterized by expression set transcription factors including master regulators Nanog Oct4. Recently, it has been shown that can be induced in somatic cells viral-mediated Oct3/4, Sox2, Klf4, c-Myc. Results Here we show...
Abstract The MECP2 (methyl‐CpG‐binding protein 2) gene has been implicated in the pathogenesis of Rett Syndrome. A rat model with a down‐regulated Mecp2 was established using recombinant lentiviral vector expressing small hairpin RNA gene. Four vectors were constructed by inserting sequences targeting To determine which resulted optimal down‐regulation, skin fibroblasts transfected four vectors, respectively. mecp2‐sh‐1 decreased expression mRNA and relative to Control group. 12–24 h after...
Adenosine kinase (ADK) is a key enzyme widely distributed in plants, playing an important role maintaining cellular energy homeostasis and regulating plant growth, development, responses to environmental stresses. However, research on ADK genes cotton (Gossypium hirsutum), economically significant crop, has been limited. This study identified 92 from four species (G. arboreum, G. raimondii, hirsutum, barbadense) using HMMER Local BLASTP methods classified them into six groups. Chromosomal...