In this study, Fourier transform infrared spectroscopy (FT-IR), gel permeation chromatograph-liquid chromatography (GPC-LC), and scanning electron microscopy (SEM) were used to analyze the molecular characteristics of fermented Dendrobium officinale polysaccharides (FDOP) by Lactobacillus delbrueckii bulgaricus. The characteristic structural peak FDOP was more prominent, showing a smaller structure, its porous structure showed better water solubility. protective effect on damage human skin...

Aging is closely related to many diseases and a long-term challenge that humans face. The oxidative damage caused by the imbalance of free radicals an important factor in aging. In this study, we investigate antioxidant antiaging activities fermented coix seed polysaccharides (FCSPs) via vitro vivo experiments. FCSPs were extracted fermenting with

UV and external environmental stimuli can cause oxidative damage to skin cells. However, the molecular mechanisms involved in cell have not been systematically clearly elucidated. In our study, an RNA-seq technique was used determine differentially expressed genes (DEGs) of UVA/H2O2-induced model. Gene Oncology (GO) clustering Kyoto Encyclopedia Genes Genomes (KEGG) Pathway analysis were performed core DEGs key signaling pathway. The PI3K-AKT pathway selected as playing a part process...

UVA can cause oxidative stress and photoaging of cells. We established a UVA-induced model human fibroblasts focused on the antioxidant anti-photoaging ability Lactobacillus plantarum fermented Rhodiola rosea. Compared with unfermented rosea, rosea has better DPPH free radical hydroxyl scavenging ability, significantly reduces content reactive oxygen species (ROS), improves level. Further studies have shown that activate Nrf2/Keap1 signaling pathway up-regulate heme oxygenase-1 (HO-1),...

Li, Z., Zhao, D., Gong, B., Xu, Y., Sun, H., Yang B. and X. Decreased Saliva Secretion Down-Regulation of AQP5 in Submandibular Gland Irradiated Rats. Radiat. Res. 165, 678–687 (2006).The molecular mechanisms radiation-induced xerostomia remain unclear. The purpose this study was to investigate the alterations aquaporins (AQPs) Na /K -ATPase irradiated rat submandibular glands test hypothesis that down-regulation expression salivary is one xerostomia. from control analyzed. mRNA level...

Ganoderma lucidum (G. lucidum) is one of China's traditional medicinal materials. G. polysaccharide has a wide range promising pharmacological applications. However, there are many kinds and they contain different polysaccharides. The biological mechanism through which polysaccharides (GLP) able to protect human skin fibroblasts (HSFs) from H2O2-induced oxidative damage still unclear.Six were obtained evaluate their free radical scavenging ability (DPPH radical, ABTS hydroxyl-free superoxide...

Damage caused by oxidative stress leads to the premature aging of cells. Mogrosides, main active components Siraitia grosvenorii, have strong antioxidant activity; however, it is unclear whether mogroside V (MV) exerts these effects in skin This was investigated present study evaluating protective MV against damage induced hydrogen peroxide (H2O2) fibroblasts.Mouse fibroblasts (MSFs) were treated with H2O2 and cell viability, total capacity, reactive oxygen species (ROS) production,...

Ectoin is an amino acid derivative that can create a balance between the osmotic pressure of cells and protect enzymes, DNA proteins, nucleic acids under extreme conditions. has also been reported to slow skin aging. However, there are few reports on protective effect oxidative damage, especially regulation PI3K/AKT-pathway-related genes at mRNA level. UVA-induced stress injury H2O2-induced two common models. Skin fibroblasts produce large number ROS following excessive UV radiation or...

Panax notoginseng is one of the most valuable traditional Chinese medicines. Polysaccharides in P. has been shown to significantly reduce incidence human diseases. However application fermentation technology not common, and mechanism action polysaccharides produced by still unclear. The specific biological mechanisms fermented (FPNP) suppresses H2O2-induced apoptosis dermal fibroblast (HDF) underlying are well understood.In this study, effects water extracted on concentration extracts were...

Long-term exposure of human skin to ultraviolet light can cause cell damage, and UVA mainly causes photoaging. The purpose this study is prove that the extracellular polysaccharide (LPL-EPS-02) isolated from a kind Lactobacillus plantarum kimchi protect repair fibroblasts after irradiation. In experiment, real-time reverse transcription-polymerase chain reaction (qRT-PCR) ELISA methods were used establish UVA-irradiated dermal fibroblast (HDF) model explore damage caused by LPL-EPS-02...

To evaluate the efficacy of dandelion fermentation broth in repairing UVB-induced skin inflammation.Detection active ingredients and water extract. The antioxidant capacity was investigated by vitro experiments. influence on content inflammatory factors interleukin-6 (IL-6), interleukin-8 (IL-8) interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), human immortalized epidermal cells (HaCaT) is discussed basis a HaCaT damage model. effects contents barrier-related proteins kallikrein-7...

The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. SS-01 strain (LP-EPS) is isolated and purified from yogurt. It a polysaccharide with branched multi-stranded structure, which exists in smooth rod-like or cloud-like state, possesses good thermal stability molecular weight of 49.68 kDa (±4.436%). LP-EPS shows high antioxidant capacity, anti-inflammatory anti-sensitizing activity during vitro experimental studies, half clearance (IC50) rates 0.449, 1.314, 2.369 mg/mL for...

A urea-selective urine-concentrating defect was found in transgenic mice deficient urea transporter (UT)-B. To determine the role of facilitated transport extrarenal organs expressing UT-B, we studied kinetics [ 14 C]urea distribution UT-B-null versus wild-type mice. After renal blood flow disrupted, selectively reduced testis Under basal conditions, total content 335.4 ± 43.8 μg 196.3 18.2 ( P < 0.01). Testis weight (6.6 0.8 mg/g body wt) significantly greater than (4.2 wt). Elongated...

UVA radiation can cause skin oxidative stress. Tremella fuciformis polysaccharides (TFPS) are the main active ingredient in Tremella. Previous studies have shown that TFPS protective effects on UVA-induced stress cells, but specific mechanism has not been clarified.This study aims to explore potential of damage human dermal fibroblasts cells.We evaluated ability against by detecting cell survival rate, content reactive oxygen species (ROS) and malondialdehyde (MDA) scavenging activity ABTS...

Hippophae rhamnoides L. has been widely used in research and application for almost two decades. While significant progress was achieved the examination of its fruits seeds, exploration utilization by-products have received relatively less attention. This study aims to address this gap by investigating effects underlying mechanisms sea buckthorn seed residues both vitro vivo. The primary objective is assess potential hydroalcoholic extract from (HYD-SBSR) prevent cell apoptosis mitigate...

Outer membrane protein A (OmpA) of Acinetobacter baumannii (A. baumannii) is associated with autophagy, which plays an important role in its pathogenicity. However, exact pathophysiological the process lung tissue cell autophagy remains unclear. In this study, animal and infection models were established by wild A. strain An OmpA knockout mutant (OmpA-/- strain. The expression levels markers histological change, viability inflammatory cytokines examined. OmpA-/- was successfully constructed....

Abstract Reactive oxygen species (ROS) accumulation in chronic skin wounds impedes the healing process, thus it is necessary to eliminate ROS from vicinity of wound time. Ascorbyl palmitate (AP) a potent antioxidant that suffers solubility constraints, which largely limits its application. This study aims improve AP's by encapsulating within 2‐hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) acquire AP/CD inclusion complex (IC). advancement facilitates development and antibacterial nanofibrous...

Saccharomycopsis fibuligera is recognized as a food-based yeast which widely used in food and drugs due to its high yield characteristics of extracellular enzymes. In this study, the proteins S. (SF-1-ECPs) are analyzed by HPLC, finding that 22.24% molecular weight SF-1-ECPs less than 2000 Da, biochemical experiments, cell experiments conducted explore significance cosmetics. The results show can enhance antioxidant enzyme activity mRNA expression within cells using Nrf2/Keap1/Mafk pathway...