A5100728617- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Ion Transport and Channel Regulation
- Epigenetics and DNA Methylation
- Mesenchymal stem cell research
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Immune cells in cancer
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Chronic Obstructive Pulmonary Disease (COPD) Research
- COVID-19 Clinical Research Studies
- Fibroblast Growth Factor Research
- Sepsis Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Respiratory viral infections research
- Influenza Virus Research Studies
- Neuroscience of respiration and sleep
- Tracheal and airway disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Lung Cancer Diagnosis and Treatment
- Electrolyte and hormonal disorders
- S100 Proteins and Annexins
Fudan University
2016-2025
Zhongshan Hospital
2016-2025
The First Affiliated Hospital, Sun Yat-sen University
2015-2025
Sun Yat-sen University
2015-2025
Shanghai Center For Bioinformation Technology
2023-2025
Shanghai Medical College of Fudan University
2021-2025
Inner Mongolia Medical University
2025
Jinshan Hospital of Fudan University
2021-2024
Huashan Hospital
2019-2024
Shanghai Clinical Research Center
2024
Abstract Recent in vivo studies indicate that mesenchymal stem cells (MSCs) may have beneficial effects the treatment of sepsis induced by bacterial infection. Administration MSCs these improved survival and enhanced clearance. The primary objective this study was to test hypothesis human possessed intrinsic antimicrobial properties. We studied effect derived from bone marrow on growth Gram-negative (Escherichia coli Pseudomonas aeruginosa) Gram-positive (Staphylococcus aureus) bacteria. as...
Enrichment of Corynebacterium tuberculostearicum caused by depletion the sinus microbiome contributes to chronic rhinosinusitis.
Mesenchymal stem cells secrete paracrine factors that can regulate lung permeability and decrease inflammation, making it a potentially attractive therapy for acute injury. However, concerns exist whether mesenchymal cells' immunomodulatory properties may have detrimental effects if targeted toward infectious causes of injury.Therefore, we tested the effect on fluid balance, bacterial clearance.We developed an Escherichia coli pneumonia model in our ex vivo perfused human to test therapeutic...
The potential therapeutic value of cell-based therapy with mesenchymal stem cells (MSC) has been reported in mouse models polymicrobial peritoneal sepsis. However, the mechanisms responsible for beneficial effects MSC have not well defined. Therefore, we tested effect intravenous bone marrow-derived human sepsis induced by gram-negative bacteria. At 48 h, survival was significantly increased mice treated compared control fibroblasts (3T3) or PBS. There were no significant differences levels...
Background: Particulate matter (PM) is a high risk factor for various respiratory diseases and triggers an inflammatory response in lung tissues. However, the molecular mechanism of PM-induced incompletely understood.
Evidence suggests that fasting exerts extensive antitumor effects in various cancers, including colorectal cancer (CRC). However, the mechanism behind this response is unclear. We investigate effect of on glucose metabolism and malignancy CRC. find upregulates expression a cholesterogenic gene, Farnesyl-Diphosphate Farnesyltransferase 1 (FDFT1), during inhibition CRC cell aerobic glycolysis proliferation. In addition, downregulation FDFT1 correlated with malignant progression poor prognosis...
Aquaporin-3 (AQP3) is a water channel expressed at the basolateral plasma membrane of kidney collecting-duct epithelial cells. The mouse AQP3 cDNA was isolated and encodes 292-amino acid water/glycerol-transporting glycoprotein in kidney, large airways, eye, urinary bladder, skin, gastrointestinal tract. gene analyzed, null mice were generated by targeted disruption. growth phenotype grossly normal except for polyuria. deletion had little effect on AQP1 or AQP4 protein expression but...
The concentration of salt in the thin layer fluid at surface large airways, airway-surface liquid (ASL), is believed to be central importance airway physiology and pathophysiology cystic fibrosis. Invasive sampling methods have yielded a wide range ASL [NaCl] from 40 180 mM. We developed novel fluorescent probes microscopy measure thickness, concentration, pH quantitatively cell-culture models trachea vivo. By rapid z-scanning confocal microscopy, thickness was 21 ± 4 μm well-differentiated...
Aquaporin-8 (AQP8) is a water-transporting protein expressed in organs of the mammalian gastrointestinal tract (salivary gland, liver, pancreas, small intestine, and colon) testes, heart, kidney, airways. We studied phenotype AQP8-null mice, mice lacking AQP8, together with AQP1 or AQP5. AQP8-knockout lacked detectable AQP8 transcript protein, had reduced water permeability plasma membranes from testes. Breeding heterozygous yielded whose number, survival, growth were not different those...
Abstract Water channel aquaporin 5 (AQP5) is highly expressed at the apical membrane of alveolar type I epithelial cells and confers high osmotic water permeability. AQP5 also in lung cancer tissue. Previous studies showed there was an up‐regulation expression tissue compared to surrounding normal In addition, associated with poor prognosis. Herein, we tested role oncogenesis development. Lung different were used study cell proliferation migration, two important parameters for tumour...
A novel system is developed for high-throughput multiplexed biomarker detection in single extracellular vesicles.
Although broad knowledge of influenza viral pneumonia has been established, the significance non-influenza respiratory viruses in community-acquired (CAP) and their impact on clinical outcomes remains unclear, especially non-immunocompromised adult population. Hospitalised immunocompetent patients with CAP were prospectively recruited from 34 hospitals mainland China. Respiratory detected by molecular methods. Comparisons conducted between infection groups. In total, 915 out 2336 enrolled...
Background Increasing evidence indicates that gut microbiota plays an important role in cancer progression. However, the underlying mechanism remains largely unknown. Here, we report broad-spectrum antibiotics (ABX) treatment leads to enhanced metastasis by alteration of microbiome composition.Methods Cancer LLC and B16-F10 cell mouse models, microarray/RNA sequencing analysis were used reveal regulatory functions microbiota-mediated circular RNA (circRNA)/microRNA (miRNA) networks may...
1 Aquaporin (AQP) water channels provide a major pathway for osmotically driven movement across epithelial and microvascular barriers in the lung. We used mice deficient each of three principal lung aquaporins, AQP1, AQP4 AQP5, to test hypothesis that aquaporins are important neonatal fluid balance, adult clearance formation oedema after acute injury. 2 Wet-to-dry weight ratios (W/D) lungs from wild-type decreased 7.9 5.7 over first hour spontaneous delivery. AQP deletion did not...
Background FoxM1 has been reported to be important in initiation and progression of various tumors. However, whether any indication for prognosis non-small cell lung cancer patients remains unclear. Methodology/Principal Findings In this study, expression tumor cells was examined first by immunohistochemistry 175 NSCLC specimens, the result which showed that overexpression significantly associated with positive smoking status (P = 0.001), poorer tissue differentiation 0.0052), higher TNM...
Cytotoxic T lymphocyte antigen 4 (CTLA4) and programmed cell death protein 1 (PD-1) are immune checkpoint proteins expressed in cells. Although CTLA4 expression was found multiple tumours including non-small lung cancer (NSCLC) tissues cells, its function tumour cells is unknown. Recently, PD-1 to be melanoma promote tumorigenesis. We that a subset of NSCLC lines subgroup within the tissues. further anti-CTLA4 antibody can induce PD-L1 expression, which mediated by EGFR pathway involving...
Airborne particulate matter (PM) is associated with increasing susceptibility to respiratory bacterial infection. Tight junctions (TJs) are protein complexes that form airway epithelial barrier against This study aimed investigate the effects of PM on TJs in response infection.The cytotoxicity BEAS-2B was evaluated. The reactive oxygen species (ROS) production measured by flow cytometry. Colony forming units (CFUs) assay and confocal microscopy were utilized evaluate number bacteria....
Abstract FGF-10 can prevent or reduce lung specific inflammation due to traumatic infectious injury. However, the exact mechanisms are poorly characterized. Additionally, effect of on lung-resident mesenchymal stem cells (LR-MSCs) has not been studied. To better characterize LR-MSCs, was intratracheally delivered into lungs rats. Three days after instillation, bronchoalveolar lavage performed and plastic-adherent were cultured, characterized then therapeutically rats LPS intratracheal...